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1. 发明申请

US20180163251A1 TARGET REGION ENRICHMENT METHOD BASED ON MULTIPLEX PCR, AND REAGENT 审中-公开
公开(公告)号：US20180163251A1
公开(公告)日：2018-06-14
申请号：US15537396
申请日：2015-12-09
申请人： BGI SHENZHEN , BGI SHENZHEN CO., LIMITED
发明人： Jing Guo , Rongrong GUO , Meiyan LI , Chunyu GENG , Hui JIANG
IPC分类号： C12Q1/6806 , C12P19/34
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/6855 , C12Q2525/155 , C12Q2531/101 , C12Q2563/131 , C12Q2563/179
摘要： Provided are a target region enrichment method based on multiplex PCR, and a reagent, the method comprising: connecting a first linker and a second linker respectively at two ends of a nucleic acid segment containing target regions to be enriched so as to obtain a linker-connected product; performing a PCR amplification on the linker-connected product using a first primer specifically bound to the first linker and a second primer specifically bound to the second linker to obtain an amplified product, the first primer or the second primer having a first affinity label; capturing a single strand having the first affinity label in the amplified product using a solid phase carrier; performing single primer linear amplification using a third primer with the captured single strand as a template; performing exponential amplification using the third primer and the first primer, with the linearly amplified product as the template, to obtain a product containing the target regions.

2. 发明申请

US20150376697A1 METHOD AND SYSTEM TO DETERMINE BIOMARKERS RELATED TO ABNORMAL CONDITION 审中-公开
标题翻译：用于确定与异常条件相关的生物标志物的方法和系统
公开(公告)号：US20150376697A1
公开(公告)日：2015-12-31
申请号：US13640448
申请日：2012-08-22
申请人： Shenghui Li , Junjie Qin , Jianfeng Zhu , Dongya Zhang , Zhuye Jie , Jun Wang , Jian Wang , Huanming Yang
发明人： Shenghui Li , Junjie Qin , Jianfeng Zhu , Dongya Zhang , Zhuye Jie , Jun Wang , Jian Wang , Huanming Yang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q2600/112
摘要： A method and system to determine biomarkers related to abnormal condition in a subject are provided, comprising:sequencing nucleic acid samples from a first and a second subject in order to obtain multiple sequences respectively consisting of the first and the second sequencing results, wherein the first subject is in the abnormal condition; and the second subject is not in the abnormal condition; and the nucleic acid samples from the first and the second subject are both isolated from the samples of the same type; and the first and the second subject belong to the same species; and determining the biomarkers related to the abnormal condition in the subject based on the difference between the first and the second sequencing results.
摘要翻译：提供了确定与受试者异常状况相关的生物标志物的方法和系统，其包括：对来自第一和第二受试者的核酸样品进行测序，以获得分别由第一和第二测序结果组成的多个序列，其中第一个受试者处于异常状况; 第二个受试者不处于异常状态; 并且来自第一和第二受试者的核酸样品均从相同类型的样品中分离; 第一和第二主题属于同一物种; 并且基于第一和第二测序结果之间的差异来确定与受试者的异常状况相关的生物标志物。

3. 发明申请

US20180195060A1 METHOD FOR CONSTRUCTING LONG FRAGMENT DNA LIBRARY 审中-公开
公开(公告)号：US20180195060A1
公开(公告)日：2018-07-12
申请号：US15567963
申请日：2016-04-14
申请人： BGI SHENZHEN , BGI SHENZHEN CO., LIMITED
发明人： Ou WANG , Cankun CHANG , Lin LIN , Hui JIANG , Wenwei ZHANG
IPC分类号： C12N15/10 , C40B50/06 , C40B40/06
CPC分类号： C12N15/1093 , C12N15/10 , C40B40/06 , C40B50/06 , C12Q2525/191 , C12Q2563/179
摘要： Disclosed is a method for constructing a long fragment DNA library, comprising the following steps: 1) breaking a long fragment DNA into target fragments of 3-10 kb by transposase, then amplifying the target fragments, and obtaining target fragment amplification products containing dUTP; 2) amplifying the dUTP in the products by removing the target fragments, fragmenting the target fragments secondarily into DNA short fragments of 300-1200 bp; 3) connecting both ends of the DNA short fragments with sequencing linker single chains A and sequencing linker single chains B respectively; and obtaining connecting sequencing linker products; and 4) PCR amplifying the connecting sequencing linker products, to obtain amplification products.

4. 发明申请

US20170234852A1 BIOMARKER COMPOSITIONS SPECIFIC TO CORONARY HEART DISEASE PATIENTS AND USES THEREOF 审中-公开
公开(公告)号：US20170234852A1
公开(公告)日：2017-08-17
申请号：US15515513
申请日：2014-09-30
申请人： BGI SHENZHEN CO., LIMITED , BGI SHENZHEN
发明人： Qiang Feng , Zhipeng Liu , Nan Meng , Jun Wang
IPC分类号： G01N33/493 , G06F19/24 , G01N30/72
CPC分类号： G01N33/493 , G01N30/02 , G01N30/62 , G01N30/7233 , G01N33/48 , G01N33/6893 , G01N2570/00 , G01N2800/324 , G01N2800/50 , G01N2800/56 , G06F19/324 , G16B40/00 , G16H50/30
摘要： The present invention relates to a disease-specific metabolite profile, and particularly to a biomarker composition obtained by screening from urine-specific metabolite profiles of coronary heart disease subjects. The present invention also relates to a use of the biomarker compositions in risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease, and to a method for risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease. The biomarker composition as provided by the present invention can be used for early diagnosis of coronary heart disease and has high sensitivity, good specificity and good application prospects.

5. 发明授权

US09359642B2 Method for preparing nucleic acid library, its uses and kits 有权
标题翻译：制备核酸文库的方法及其用途和试剂盒
公开(公告)号：US09359642B2
公开(公告)日：2016-06-07
申请号：US14352492
申请日：2012-10-16
申请人： BGI Shenzhen Co., Limited , BGI Shenzhen
发明人： Xuyang Yin , Chunlei Zhang , Hui Jiang , Xiuqing Zhang , Shengpei Chen
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6874 , C12N15/1093 , C12Q1/6853 , C12Q1/686 , C12Q1/6869 , C12Q2523/125 , C12Q2525/179 , C12Q2525/191 , C12Q2531/113 , C12Q2537/159
摘要： Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.
摘要翻译：提供构建核酸文库的方法，确定核酸样品的核酸序列的方法及其试剂盒。构建核酸文库的方法包括以下步骤：使核酸样品进行DOP-PCR扩增，得到第一PCR扩增产物; 使用DOP-Amp引物对第一PCR扩增产物进行第二次PCR扩增，得到第二PCR扩增产物; 并对第二PCR扩增产物进行衔接连接PCR，得到第三PCR扩增产物，其中第三PCR扩增产物构成核酸文库。

6. 发明申请

US20140206006A1 SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF 审中-公开
标题翻译：单细胞分类方法，基因筛选方法及其装置
公开(公告)号：US20140206006A1
公开(公告)日：2014-07-24
申请号：US14239650
申请日：2012-08-24
申请人： Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
发明人： Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
IPC分类号： G06F19/22
CPC分类号： G16B30/00 , C12Q1/6827 , C12Q1/6869 , G16B20/00
摘要： Provided are a single cell classification method, a gene screening method and a device for implementing the method. In that, the single cell classification method includes the following steps: sequencing the whole genomes of a plurality of single cell samples from the same group, respectively, so as to obtain reads from each single cell sample; aligning the reads from each single cell sample to the sequence of a reference genome, respectively, and performing data filtering on said reads; on the basis of the filtered reads, determining a consistent genotype of each single cell sample, in which consistent genotypes of all the single cell samples constitute an SNP dataset of said group; aimed at said each single cell, on the basis of the SNP dataset of said group, determining a corresponding genotype for each cell at a site corresponding to a position in an SNP dataset of the reference genome; and selecting an SNP site associated with cell mutation, and on the basis of the genotype of said single cell at the site, classifying said single cell.
摘要翻译：提供单细胞分类方法，基因筛选方法和实施该方法的装置。其中，单细胞分类方法包括以下步骤：从同一组分别测序多个单细胞样品的全基因组，从而获得每个单细胞样品的读数; 将每个单个细胞样本的读数分别对准参考基因组的序列，并对所述读取执行数据过滤; 基于经过滤的读数，确定每个单细胞样品的一致的基因型，其中所有单细胞样品的一致基因型构成所述组的SNP数据集; 针对所述每个单细胞，基于所述组的SNP数据集，在对应于参照基因组的SNP数据集中的位置的位点处确定每个细胞的相应基因型; 并选择与细胞突变相关的SNP位点，并且基于所述单细胞在所述位点处的基因型，对所述单细胞进行分类。

7. 发明申请

US20130288907A1 DNA library, preparation method thereof, and device for detecting SNPs 有权
标题翻译： DNA文库，其制备方法以及检测SNP的装置
公开(公告)号：US20130288907A1
公开(公告)日：2013-10-31
申请号：US13989031
申请日：2011-09-21
申请人： Ye Du
发明人： Ye Du
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12N15/1093 , C12Q1/6869 , C12Q1/6883 , C12Q2521/313 , C12Q2525/191 , C40B50/06
摘要： A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.
摘要翻译：可以提供DNA文库及其制备方法，确定DNA序列信息的方法，用于检测SNP的装置和试剂盒，以及用于基因分型的方法。制备DNA文库的方法可包括以下步骤：使用限制性内切核酸酶消化基因组DNA样品以获得消化产物，其中所述限制性内切核酸酶包含选自Mbo II和Tsp 45I中的至少一种; 分离消化的产物以获得长度为100bp至1,000bp的DNA片段; 终止修复DNA片段以获得最终修复的DNA片段; 在末端修复的DNA片段的末端添加碱基A以获得具有末端碱基A的DNA片段; 并将具有末端碱基A的DNA片段与测序接头连接以获得DNA文库。

8. 发明公开

US20240209439A1 NUCLEOTIDE ANALOGUE FOR SEQUENCING 审中-公开
公开(公告)号：US20240209439A1
公开(公告)日：2024-06-27
申请号：US18284054
申请日：2022-03-31
申请人： BGI SHENZHEN , BGI SHENZHEN CO. LTD.
发明人： Xun XU , Bo TENG , Wenwei ZHANG , Liang SHEN , Shitian ZHUO , Shengyi YAN , Nanfeng GAO , Yonghui ZHANG , Jie ZHAO , Sha LIAO , Ming WANG , Shaoqiao ZHANG , Ao CHEN , Handong LI
IPC分类号： C12Q1/6874 , C07H19/10 , C07H19/14 , C07H19/20 , C12Q1/6806 , C12Q1/6876
CPC分类号： C12Q1/6874 , C07H19/10 , C07H19/14 , C07H19/20 , C12Q1/6876 , C12Q1/6806
摘要： The present invention relates to the field of nucleic acid sequencing. Specifically, provided in the present invention is a nucleotide analogue for sequencing. The nucleotide analogue carries a linker on the base or the ribose ring 3′-OH, and can be used for NGS sequencing. The present invention further relates to a kit containing the nucleotide analogue, and a sequencing method based on the nucleotide analogue.

9. 发明申请

US20230032409A1 Method for Information Encoding and Decoding, and Method for Information Storage and Interpretation 有权
公开(公告)号：US20230032409A1
公开(公告)日：2023-02-02
申请号：US17763221
申请日：2019-09-24
申请人： BGI SHENZHEN , BGI SHENZHEN CO., LIMITED
发明人： Shihong CHEN , Xiaoluo HUANG , Zhi PING , Tao LIN , Chen CHAI , Yue SHEN , Xun XU , Huanming YANG
IPC分类号： G06F3/06
摘要： A method for information encoding and decoding, and method for information storage and interpretation are provided. The information encoding method includes: first binary information and second binary information as well as a first encoding rule and a second encoding rule are obtained; a first output candidate symbol corresponding to a current input of the first binary information is obtained and a second output candidate symbol corresponding to a current input of the second binary information is obtained, and an intersection of the first output candidate symbol and the second output candidate symbol is taken as an output corresponding to a current input; and an output symbol corresponding to each binary bit of the first binary information and the second binary information is sequentially determined through the first encoding rule and the second encoding rule, as to obtain an encoding sequence formed by a plurality of the output symbols.

10. 发明申请

US20140296084A1 Method for preparing nucleic acid library, its uses and kits 有权
标题翻译：制备核酸文库的方法及其用途和试剂盒
公开(公告)号：US20140296084A1
公开(公告)日：2014-10-02
申请号：US14352492
申请日：2012-10-16
申请人： BGI Shenzhen Co., Limited , BGI Shenzhen
发明人： Xuyang Yin , Chunlei Zhang , Hui Jiang , Xiuqing Zhang , Shengpei Chen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/1093 , C12Q1/6853 , C12Q1/686 , C12Q1/6869 , C12Q2523/125 , C12Q2525/179 , C12Q2525/191 , C12Q2531/113 , C12Q2537/159
摘要： Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.
摘要翻译：提供构建核酸文库的方法，确定核酸样品的核酸序列的方法及其试剂盒。构建核酸文库的方法包括以下步骤：使核酸样品进行DOP-PCR扩增，得到第一PCR扩增产物; 使用DOP-Amp引物对第一PCR扩增产物进行第二次PCR扩增，得到第二PCR扩增产物; 并对第二PCR扩增产物进行衔接连接PCR，得到第三PCR扩增产物，其中第三PCR扩增产物构成核酸文库。

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