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1. 发明授权

US07875825B2 Plasma generating equipment 有权
标题翻译：等离子发生设备
公开(公告)号：US07875825B2
公开(公告)日：2011-01-25
申请号：US10593771
申请日：2005-03-25
申请人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
发明人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
IPC分类号： B23K9/02 , G01J3/30
CPC分类号： G01N21/68 , G01N21/67 , G01N21/69
摘要： A method for generating plasma and a method for elemental analysis, each comprising the steps of providing a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; filling the flow channel and the narrow portion with a conductive liquid, and thereafter applying an electric field to the narrow portion, to conduct the electric field through the narrow portion, thereby generating plasma at the narrow portion. An apparatus for generating plasma, the apparatus for generating plasma comprising a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; and a means of applying an electric field to the narrow portion to conduct the electric field through the narrow portion; and an apparatus for emission spectroscopic analysis comprising the apparatus for generating plasma as defined above.
摘要翻译：一种用于产生等离子体的方法和元素分析方法，每个方法包括以下步骤：在由绝缘材料制成的流动通道中提供窄部分，所述窄部分的横截面积明显小于所述绝缘材料的横截面面积流通道用导电液体填充流路和狭窄部分，然后向狭窄部分施加电场，使电场通过狭窄部分，从而在狭窄部分产生等离子体。一种用于产生等离子体的装置，所述用于产生等离子体的装置包括由绝缘材料制成的流动通道中的窄部分，所述窄部分具有明显小于所述流动通道的横截面积的横截面面积; 以及向狭窄部分施加电场以通过所述狭窄部分传导电场的装置; 以及用于发射光谱分析的装置，其包括如上所述产生等离子体的装置。

2. 发明申请

US20070164003A1 Plasma generating equipment 有权
标题翻译：等离子发生设备
公开(公告)号：US20070164003A1
公开(公告)日：2007-07-19
申请号：US10593771
申请日：2005-03-25
申请人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
发明人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
IPC分类号： B23K9/00 , B23K9/02
CPC分类号： G01N21/68 , G01N21/67 , G01N21/69
摘要： A method for generating plasma and a method for elemental analysis, each comprising the steps of providing a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; filling the flow channel and the narrow portion with a conductive liquid, and thereafter applying an electric field to the narrow portion, to conduct the electric field through the narrow portion, thereby generating plasma at the narrow portion. An apparatus for generating plasma, the apparatus for generating plasma comprising a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; and a means of applying an electric field to the narrow portion to conduct the electric field through the narrow portion; and an apparatus for emission spectroscopic analysis comprising the apparatus for generating plasma as defined above.
摘要翻译：一种用于产生等离子体的方法和元素分析方法，每个方法包括以下步骤：在由绝缘材料制成的流动通道中提供窄部分，所述窄部分的横截面积明显小于所述绝缘材料的横截面面积流通道用导电液体填充流路和狭窄部分，然后向狭窄部分施加电场，使电场通过狭窄部分，从而在狭窄部分产生等离子体。一种用于产生等离子体的装置，所述用于产生等离子体的装置包括由绝缘材料制成的流动通道中的窄部分，所述窄部分具有明显小于所述流动通道的横截面积的横截面面积; 以及向狭窄部分施加电场以通过所述狭窄部分传导电场的装置; 以及用于发射光谱分析的装置，其包括如上所述产生等离子体的装置。

3. 发明授权

US09222890B2 Plasma spectrometer 有权
标题翻译：等离子体光谱仪
公开(公告)号：US09222890B2
公开(公告)日：2015-12-29
申请号：US14000610
申请日：2012-01-13
申请人： Yoshinobu Kohara , Yuzuru Takamura
发明人： Yoshinobu Kohara , Yuzuru Takamura
IPC分类号： G01J3/30 , G01N21/67 , G01N21/69 , G01J3/02
CPC分类号： G01N21/67 , G01J3/02 , G01N21/69 , G01N2201/08
摘要： To improve detection sensitivity, detection accuracy, and reproducibility when electric discharge is caused in a sample solution to perform analysis with light emission in the plasma, a flow channel 101 provided with a narrow part is filled with a conductive sample solution, plasma is generated in bubbles formed by applying an electric field to the flow channel, and a region other than the narrow part in the flow channel is set as a measurement object region to measure light emission.
摘要翻译：为了提高检测灵敏度，检测精度和在样品溶液中发生放电以进行等离子体中的发光分析的再现性，设置有狭窄部分的流路101填充有导电性样品溶液，将通过对流路施加电场而形成的气泡，将流路中的狭窄部以外的区域设定为测量对象区域，以测量发光。

4. 发明申请

US20110244476A1 METHOD FOR EVALUATION OF QUALITY OF BLOOD SAMPLE 有权
标题翻译：评估血液样品质量的方法
公开(公告)号：US20110244476A1
公开(公告)日：2011-10-06
申请号：US12744064
申请日：2008-11-25
申请人： Koutarou Idegami , Takashi Yoneda , Yuzuru Takamura
发明人： Koutarou Idegami , Takashi Yoneda , Yuzuru Takamura
IPC分类号： G01N33/74 , G01N33/53
CPC分类号： G01N33/54366 , G01N33/558 , G01N33/9493
摘要： A method for evaluating the quality of a blood sample, comprising the steps of: mixing labeled anti-cortisol antibodies with a blood sample to be subjected, to conduct the antigen-antibody reaction of the labeled anti-cortisol antibody with cortisol contained in the blood sample, developing a mixture obtained in the above step on an immunochromatographic test strip having a substrate on which cortisol is immobilized to cause the antigen-antibody reaction of the labeled anti-cortisol antibodies which are free in the mixture with the cortisol immobilized on the substrate, thereby bonding the antibody to the cortisol, determining the amount of the labeled anti-cortisol antibodies bonded to the cortisol in the above step, and evaluating whether or not the blood sample has a quality suitable for suprarenal vein sampling test on the basis of the amount of the labeled anti-cortisol antibodies determined in the above step.
摘要翻译：一种用于评估血液样品质量的方法，包括以下步骤：将标记的抗皮质醇抗体与待进行血液样品混合，进行标记的抗皮质醇抗体与血液中包含的皮质醇的抗原 - 抗体反应样品，将在上述步骤中获得的混合物在具有固定有皮质醇的底物的免疫色谱试纸上培养，引起与固定在底物上的皮质醇混合物中游离的标记抗皮质醇抗体的抗原 - 抗体反应从而将抗体结合到皮质醇上，确定在上述步骤中与皮质醇结合的标记的抗皮质醇抗体的量，并且基于上述步骤评估血液样品是否具有适合于肾上静脉取样试验的质量在上述步骤中测定的标记的抗皮质醇抗体的量。

5. 发明申请

US20130072402A1 METHOD FOR COLLECTING NUCLEATED RED BLOOD CELLS VIA DENSITY-GRADIENT CENTRIFUGATION UTILIZING CHANGES IN BLOOD CELL DENSITY 有权
标题翻译：通过密度梯度离心收集核型红血细胞的方法利用血细胞密度变化
公开(公告)号：US20130072402A1
公开(公告)日：2013-03-21
申请号：US13700947
申请日：2011-02-21
申请人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
发明人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
IPC分类号： C12N5/078
CPC分类号： C12N5/0641 , B03D3/00 , C12N5/0087 , C12Q1/6806 , C12Q1/6883 , C12Q2600/118 , C12Q2600/158 , G01N33/491 , G01N33/80 , C12Q2523/32 , C12Q2527/15 , C12Q2543/10
摘要： This invention provides a method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.
摘要翻译：本发明提供了一种用于浓缩和收集母体血液中含有的少量胎儿有核红细胞的方法。用于从母体血液中浓缩和收集有核红细胞的方法包括：（i）使母体血液进行第一密度梯度离心并收集含有核红细胞的细胞部分; （ii）处理含有核红细胞的细胞部分，以选择性地改变有核红细胞的密度与白细胞的密度; 和（iii）将含有有核红细胞的经处理的细胞级分进行第二密度梯度离心，以收集含有核红细胞的级分。

6. 发明授权

US09334477B2 Method for collecting nucleated red blood cells via density-gradient centrifugation utilizing changes in blood cell density 有权
标题翻译：通过使用血细胞密度变化的密度梯度离心法收集有核红细胞的方法
公开(公告)号：US09334477B2
公开(公告)日：2016-05-10
申请号：US13700947
申请日：2011-02-21
申请人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
发明人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
IPC分类号： B03D3/00 , C12N5/078 , C12N5/00 , G01N33/49 , G01N33/80 , C12Q1/68
CPC分类号： C12N5/0641 , B03D3/00 , C12N5/0087 , C12Q1/6806 , C12Q1/6883 , C12Q2600/118 , C12Q2600/158 , G01N33/491 , G01N33/80 , C12Q2523/32 , C12Q2527/15 , C12Q2543/10
摘要： Method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.
摘要翻译：浓缩和收集母体血液中少量胎儿有核红细胞的方法。用于从母体血液中浓缩和收集有核红细胞的方法包括：（i）使母体血液进行第一密度梯度离心并收集含有核红细胞的细胞部分; （ii）处理含有核红细胞的细胞部分，以选择性地改变有核红细胞的密度与白细胞的密度; 和（iii）将含有有核红细胞的经处理的细胞级分进行第二密度梯度离心，以收集含有核红细胞的级分。

7. 发明申请

US20120301867A1 RECOVERING NUCLEATED RED BLOOD CELLS AND METHOD FOR CONCENTRATING AND RECOVERING NUCLEATED RED BLOOD CELLS 审中-公开
标题翻译：恢复核红细胞和浓缩和回收核红细胞的方法
公开(公告)号：US20120301867A1
公开(公告)日：2012-11-29
申请号：US13393854
申请日：2010-09-02
申请人： Takeshi Kumo , Yuzuru Takamura , Haruo Takabayashi
发明人： Takeshi Kumo , Yuzuru Takamura , Haruo Takabayashi
IPC分类号： C12M1/26 , C12N5/078
CPC分类号： B01L3/502746 , B01L3/502723 , B01L3/502738 , B01L3/502753 , B01L2200/0652 , B01L2300/0864 , B01L2400/0638 , B01L2400/0655 , B01L2400/082
摘要： Provided is a chip or the like having a particulate concentrating mechanism such as a mechanism that can selectively concentrate nucleated red blood cells contained in maternal blood and derived from a fetus and can collect the concentrated liquid rich in nucleated red blood cells, and also provided is a nucleated red blood cell concentrating/collecting method. A micro-channel chip for concentrating nucleated red blood cells has an inlet-side channel, an outlet-side channel, and a separation narrow channel provided between the inlet-side channel and the outlet-side channel. The separation narrow channel has an inner wall having a dimension through which non-nucleated red blood cells easily pass and nucleated red blood cells hardly pass, and has a means for deforming or moving part of the inner wall of the channel to have a dimension through which nucleated red blood cells easily pass. The following are also provided: a method for collecting a liquid in which nucleated red blood cells obtained by using this chip are concentrated; and a micro-channel chip for concentrating particulates other than for concentrating nucleated red blood cells.
摘要翻译：提供了具有颗粒聚集机构的芯片等，其可以选择性地浓缩母体血液中衍生的来自胎儿的有核红细胞，并且可以收集富含有核红细胞的浓缩液，并且还提供有核红细胞浓缩/收集方法。用于浓缩有核红细胞的微通道芯片具有入口侧通道，出口侧通道和设置在入口侧通道和出口侧通道之间的分离窄通道。分离窄通道具有内壁，该内壁具有非成核红细胞容易通过且有核的红细胞难以通过的尺寸，并且具有使通道内壁的一部分变形或移动以使其具有尺寸的装置其核红细胞容易通过。还提供以下方法：收集通过使用该芯片得到的有核红细胞的液体的收集方法; 以及用于浓缩除了用于浓缩有核红细胞之外的微粒的微通道芯片。

8. 发明授权

US08927219B2 Method for evaluation of quality of blood sample 有权
标题翻译：血液样品质量评估方法
公开(公告)号：US08927219B2
公开(公告)日：2015-01-06
申请号：US12744064
申请日：2008-11-25
申请人： Koutarou Idegami , Takashi Yoneda , Yuzuru Takamura
发明人： Koutarou Idegami , Takashi Yoneda , Yuzuru Takamura
IPC分类号： G01N33/53 , G01N33/74 , G01N33/558 , G01N33/94
CPC分类号： G01N33/54366 , G01N33/558 , G01N33/9493
摘要： A method for evaluating the quality of a blood sample, comprising the steps of: mixing labeled anti-cortisol antibodies with a blood sample to be subjected, to conduct the antigen-antibody reaction of the labeled anti-cortisol antibody with cortisol contained in the blood sample, developing a mixture obtained in the above step on an immunochromatographic test strip having a substrate on which cortisol is immobilized to cause the antigen-antibody reaction of the labeled anti-cortisol antibodies which are free in the mixture with the cortisol immobilized on the substrate, thereby bonding the antibody to the cortisol, determining the amount of the labeled anti-cortisol antibodies bonded to the cortisol in the above step, and evaluating whether or not the blood sample has a quality suitable for suprarenal vein sampling test on the basis of the amount of the labeled anti-cortisol antibodies determined in the above step.
摘要翻译：一种用于评估血液样品质量的方法，包括以下步骤：将标记的抗皮质醇抗体与待进行血液样品混合，进行标记的抗皮质醇抗体与血液中含有的皮质醇的抗原 - 抗体反应样品，将在上述步骤中获得的混合物在具有固定有皮质醇的底物的免疫色谱试纸上培养，引起与固定在底物上的皮质醇混合物中游离的标记抗皮质醇抗体的抗原 - 抗体反应从而将抗体结合到皮质醇上，确定在上述步骤中与皮质醇结合的标记的抗皮质醇抗体的量，并且基于上述步骤评估血液样品是否具有适合于肾上静脉取样试验的质量在上述步骤中测定的标记的抗皮质醇抗体的量。

9. 发明申请

US20130321803A1 PLASMA SPECTROMETER 有权
标题翻译：等离子体光谱仪
公开(公告)号：US20130321803A1
公开(公告)日：2013-12-05
申请号：US14000610
申请日：2012-01-13
申请人： Yoshinobu Kohara , Yuzuru Takamura
发明人： Yoshinobu Kohara , Yuzuru Takamura
IPC分类号： G01N21/67
CPC分类号： G01N21/67 , G01J3/02 , G01N21/69 , G01N2201/08
摘要： To improve detection sensitivity, detection accuracy, and reproducibility when electric discharge is caused in a sample solution to perform analysis with light emission in the plasma, a flow channel 101 provided with a narrow part is filled with a conductive sample solution, plasma is generated in bubbles formed by applying an electric field to the flow channel, and a region other than the narrow part in the flow channel is set as a measurement object region to measure light emission.
摘要翻译：为了提高检测灵敏度，检测精度和在样品溶液中发生放电以进行等离子体中的发光分析的再现性，设置有狭窄部分的流路101填充有导电性样品溶液，将通过对流路施加电场而形成的气泡，将流路中的狭窄部以外的区域设定为测量对象区域，以测量发光。

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