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    • 3. 发明授权
    • Gaming system that pays out a progressive bonus using a lottery
    • 游戏系统,使用彩票支付进步奖金
    • US5645486A
    • 1997-07-08
    • US518342
    • 1995-08-23
    • Yuji NagaoKeiji Yano
    • Yuji NagaoKeiji Yano
    • A63F3/00G07F17/32A63F9/24
    • G07F17/32A63F2003/0017
    • A progressive gaming system pays out a progressively increasing bonus to a player who wins a game with a predetermined winning combination. The progressive gaming system includes a plurality of gaming controllers which detect when a player gets one of a plurality of predetermined winning combinations and pays out a predetermined number of coins or tokens according to the winning combination. Each of the gaming controllers counts coins or tokens bet by the player before the game is started and outputs an indication signal of the amount of the bet coins or tokens. The progressive gaming system further includes an master controller operatively connected to the plurality of gaming controllers, which adds a predetermined percentage of the bet coins or tokens reported by each of the gaming controllers to a bonus value, performs a lottery when the player gets a predetermined winning combination, and controls each of the gaming controllers to pay out coins or tokens corresponding to a predetermined reward for the predetermined winning combination and saved bonus value.
    • 渐进式游戏系统对于以预定的获胜组合赢得游戏的玩家支付逐渐增加的奖金。 渐进式游戏系统包括多个游戏控制器,用于检测玩家何时获得多个预定获胜组合中的一个并根据获胜组合支付预定数量的硬币或令牌。 每个游戏控制器在游戏开始之前计数玩家投注的硬币或令牌,并输出下注硬币或令牌的量的指示信号。 逐行游戏系统还包括可操作地连接到多个游戏控制器的主控制器,其将由游戏控制器中的每一个报告的下注硬币或令牌的预定百分比增加到奖金值,当玩家获得预定的游戏控制器时执行彩票 并且控制每个游戏控制器以支付对应于预定获胜组合的预定奖励的硬币或令牌,并且保存奖励值。
    • 8. 发明授权
    • Escherichia coli Candida maltosa Saccharomyces cerevisiae shuttle
vectors and method for making
    • 大肠杆菌麦芽糖酵母(Saccharomyces cerevisiae)梭菌载体及其制备方法
    • US4879230A
    • 1989-11-07
    • US813193
    • 1985-12-24
    • Masamichi TakagiKeiji YanoIchiro ShibuyaMinoru Morikawa
    • Masamichi TakagiKeiji YanoIchiro ShibuyaMinoru Morikawa
    • C12N15/81
    • C12N15/81
    • The present invention relates to plasmids whose hosts can be Escherichia coli and some kinds of yeasts, namely, shuttle vectors, as well as to processes for producing said plasmids. There are provided in the present invention (1) plasmids containing an autonomously replicating sequence of Candida maltosa, Leu 2 gene derived from Saccharomyces cerevisiae and an ampicillin resistance gene and (2) plasmids further containing a tetracycline resistance gene as well as the genes described in (1).The plasmids (shuttle vectors) of the present invention can be utilized as follows. A useful foreign gene is inserted into plasmids of the present invention; using the resulting new plasid, Escherichia coli is transformed and cultured in order to obtain the plasmid in a large amount; and using this plasmid, Saccharomyces cerevisiae or Candida maltosa as a host is allowed to produce useful substances such as hormones and enzymes on a large scale.
    • 本发明涉及其宿主可以是大肠杆菌和一些种类的酵母的质粒,即穿梭载体,以及生产所述质粒的方法。 在本发明中提供了(1)含有自愿复制的假丝酵母(Candida maltosa)的质粒,来源于酿酒酵母的Leu 2基因和氨苄青霉素抗性基因的质粒和(2)进一步含有四环素抗性基因的质粒以及描述于 (1)。 本发明的质粒(穿梭载体)可以如下使用。 将有用的外源基因插入本发明的质粒中; 使用所得的新的质粒,转化和培养大肠杆菌以获得大量的质粒; 使用该质粒,可以将酿酒酵母或麦芽假丝酵母作为宿主大量产生有用的物质如激素和酶。