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1. 发明授权

US5612192A DNA base sequence containing regions involved in the production and secretion of a protein, recombinant DNA including the whole or a part of the DNA base sequence, and method of producing proteins by use of the recombinant DNA 失效
标题翻译：含有涉及蛋白质的生成和分泌的区域的DNA碱基序列，包含全部或部分DNA碱基序列的重组DNA，以及使用重组DNA产生蛋白质的方法
公开(公告)号：US5612192A
公开(公告)日：1997-03-18
申请号：US359863
申请日：1994-12-20
申请人： Yoshio Furutani , Hiroaki Shimada , Izumi Mita , Kazuaki Manabe , Masaru Honjo , Noboru Tomioka
发明人： Yoshio Furutani , Hiroaki Shimada , Izumi Mita , Kazuaki Manabe , Masaru Honjo , Noboru Tomioka
IPC分类号： C12N9/54 , C12N15/75 , C12N15/00 , C12P21/00
CPC分类号： C12N15/75 , C12N9/54 , C07K2319/02 , C07K2319/036
摘要： A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.
摘要翻译：克隆解淀粉芽孢杆菌的中性蛋白酶基因，该基因包含启动子区，核糖体结合区，参与中性蛋白酶分泌的区域，由中性蛋白酶的结构基因组成的区域和终止区。通过培养用重组DNA转化的宿主细菌，每个区域可用作构建用于生产蛋白质的重组DNA的材料。例如，可以通过培养用包含pUB110和中性蛋白酶基因的重组DNA转化的枯草芽孢杆菌来实现大量的中性蛋白酶的细胞外产生。

2. 发明授权

US4824782A DNA base sequence, method for preparing a recombinant plasmid including the DNA base sequence, and breeding method for enhancing the protein-secreting ability of a microorganism by introducing thereinto the recombinant plasmid 失效
标题翻译： DNA碱基序列，包含DNA碱基序列的重组质粒的制备方法以及通过将重组质粒引入微生物的蛋白质分泌能力的育种方法
公开(公告)号：US4824782A
公开(公告)日：1989-04-25
申请号：US180691
申请日：1988-04-08
申请人： Yoshio Furutani , Noboru Tomioka , Masaru Honjo , Kazuaki Manabe , Hiroaki Shimada
发明人： Yoshio Furutani , Noboru Tomioka , Masaru Honjo , Kazuaki Manabe , Hiroaki Shimada
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/56 , C12N15/00 , C12N15/67 , C12N15/75 , C12P21/00 , C12R1/07 , C12N7/00
CPC分类号： C12N9/54 , C12N15/67 , C12N15/75
摘要： This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.
摘要翻译：本发明涉及能够增加微生物分泌蛋白质的DNA碱基序列及其衍生序列; 包含全部或部分所述DNA碱基序列的重组质粒; 一种重组质粒的制备方法，其中，在将包含所需DNA碱基序列的重组DNA导入其中的微生物在克隆过程中被分离的情况下，可以通过从所述重组质粒中分泌出的蛋白质的量来有效地选择合适的转化体细胞，特别是酶蛋白的活性作为指标; 以及微生物育种方法，其包括将重组质粒导入微生物以增加微生物分泌的蛋白质的量。

3. 发明授权

US4731327A Method for stabilizing recombinant DNA molecules and enhancing the expression thereof in strains of Bacillus 失效
标题翻译：用于稳定重组DNA分子并增强其在芽孢杆菌菌株中的表达的方法
公开(公告)号：US4731327A
公开(公告)日：1988-03-15
申请号：US896583
申请日：1986-08-18
申请人： Yoshio Furutani , Masaru Honjo , Kazuaki Manabe , Hiroaki Shimada , Noboru Tomioka
发明人： Yoshio Furutani , Masaru Honjo , Kazuaki Manabe , Hiroaki Shimada , Noboru Tomioka
IPC分类号： C12N15/09 , C12N9/54 , C12N15/67 , C12N15/75 , C12N15/00 , C12P21/00
CPC分类号： C12N15/75 , C12N15/67 , C12N9/54
摘要： A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperature-sensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal DNA replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.
摘要翻译：重组DNA分子，其一部分包括其复制独立于作为宿主的芽孢杆菌属的温度敏感突变菌株的温度敏感性突变基因启动的载体导入宿主。然后在不完全抑制染色体DNA复制的温度下培养宿主。通过该方法，重组DNA分子可以在宿主中稳定，并且其表达可以增强。

4. 发明授权

US5084383A Bacillus subtilis strain whose extracellular protease activities are reduced, method for obtaining the strain and method for secreting proteins by using the strain 失效
标题翻译：减少细胞外蛋白酶活性的BACILLUS SUBTILIS菌株，获得菌株的方法和使用菌株分泌蛋白质的方法
公开(公告)号：US5084383A
公开(公告)日：1992-01-28
申请号：US553356
申请日：1990-07-18
申请人： Yoshio Furutani , Masaru Honjo , Akira Nakayama , Koichi Kawamura , Hiroaki Shimada , Izumi Mita , Akiko Akaoka
发明人： Yoshio Furutani , Masaru Honjo , Akira Nakayama , Koichi Kawamura , Hiroaki Shimada , Izumi Mita , Akiko Akaoka
IPC分类号： C12N1/20 , C07K14/32 , C07K14/61 , C12N1/21 , C12N9/54 , C12N15/09 , C12N15/67 , C12N15/75 , C12P21/00 , C12P21/02 , C12R1/07 , C12R1/125
CPC分类号： C12N15/75 , C07K14/32 , C07K14/61 , C12N15/67
摘要： Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain.The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium.For example, the accumulation level of human growth hormone secreted by a B. subtilis MT-430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activity-deficient strain.
摘要翻译：通过将用于刺激细胞外蛋白酶水平的基因引入菌株的基因组DNA，可以进一步降低枯草芽孢杆菌菌株的残留细胞外蛋白酶活性。细胞外蛋白酶活性进一步降低的所得菌株用用于分泌所需蛋白质的重组质粒转化，并且所需蛋白质可以有效地产生并大量积累在培养基中。可以容易地从培养基中回收积累的所需蛋白质。例如，由于上述用于还原的人类生长激素的表达和分泌，由携带重组DNA phGH427的枯草芽孢杆菌MT-430菌株分泌的人生长激素用于人生长激素的表达和分泌的累积水平可增加5至10倍细胞外蛋白酶活性缺陷型菌株的残留细胞外蛋白酶活性。

5. 发明授权

US5015574A DNA sequence involved in gene expression and protein secretion, expression-secretion vector including the DNA sequence and the method of producing proteins by using the expression-secretion vector 失效
标题翻译：涉及基因表达和蛋白质分泌的DNA序列，包括DNA序列的表达分泌载体和通过使用表达分泌载体产生蛋白质的方法
公开(公告)号：US5015574A
公开(公告)日：1991-05-14
申请号：US932587
申请日：1986-11-20
申请人： Yoshio Furutani , Akira Nakayama , Masaru Honjo , Hiroaki Shimada , Kouichi Kawamura , Izumi Mita , Akiko Akaoka
发明人： Yoshio Furutani , Akira Nakayama , Masaru Honjo , Hiroaki Shimada , Kouichi Kawamura , Izumi Mita , Akiko Akaoka
IPC分类号： C12N15/09 , C07H21/04 , C07K14/565 , C07K14/61 , C12N1/21 , C12N9/28 , C12N9/54 , C12N15/00 , C12N15/67 , C12N15/75 , C12P21/02 , C12R1/07
CPC分类号： C12N9/2417 , C07K14/565 , C07K14/61 , C12N15/67 , C12N15/75 , C12N9/54
摘要： A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.
摘要翻译：通过使用包含DNA片段的DNA序列构建表达分泌载体，所述DNA序列通过使由编码芽孢杆菌属的细菌的细胞外基因的基因的表达所涉及的区域和涉及的区域因此分泌的酶在涉及分泌的区域中被表达为可以增强依赖于DNA片段的蛋白质的细胞外产生的缺失。通过将包含编码所需蛋白质的基因的DNA片段插入表达分泌载体并随后培养转化的宿主而形成的重组DNA分子转化宿主细菌，可以大量的所需蛋白质的细胞外产生完成了

6. 发明申请

US20070115390A1 Television broadcast receiving apparatus, door phone apparatus, and interphone system 审中-公开
标题翻译：电视广播接收装置，门电话装置和对讲机系统
公开(公告)号：US20070115390A1
公开(公告)日：2007-05-24
申请号：US11599348
申请日：2006-11-15
申请人： Hiroyuki Makara , Hiroaki Shimada
发明人： Hiroyuki Makara , Hiroaki Shimada
IPC分类号： H04N7/00 , H04N5/44
CPC分类号： H04N7/186
摘要： Disclosed is an interphone system that uses a home-use television broadcast receiving apparatus capable of receiving instructions for dealing with a visitor. According to the invention, a television includes a sending/receiving section for sending and receiving signals to and from a door phone apparatus, a storage section in which response information is stored as text data, and an audio synthesis processor which converts text data into an audio signal. An audio signal based on response information (e.g., “Please come in”) selected with the television is sent to the door phone apparatus and outputted from a door phone slave unit installed at the entrance of a house. Thus, it becomes possible to deal with a visitor by operating the television.
摘要翻译：公开了一种对讲机系统，其使用能够接收与访问者进行交易的指令的家用电视广播接收装置。根据本发明，电视机包括用于向门电话装置发送信号和从门电话装置接收信号的发送/接收部分，将响应信息作为文本数据存储的存储部分，以及将文本数据转换为音频信号。基于用电视机选择的响应信息（例如“请进入”）的音频信号被发送到门电话设备并从安装在房屋入口处的门电话从单元输出。因此，可以通过操作电视来处理访问者。

7. 发明申请

US20060010519A1 Method for producing ubiquinone-10 in plant 失效
标题翻译：植物中泛醌-10的生产方法
公开(公告)号：US20060010519A1
公开(公告)日：2006-01-12
申请号：US11119351
申请日：2005-04-29
申请人： Koichi Kadowaki , Sakiko Takahashi , Makoto Kawamukai , Hiroaki Shimada
发明人： Koichi Kadowaki , Sakiko Takahashi , Makoto Kawamukai , Hiroaki Shimada
IPC分类号： A01H1/00 , C12N15/82 , C12N9/10
CPC分类号： C12N9/1085 , C12N15/8243
摘要： A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.
摘要翻译：提供了表达大量泛醌-10的植物和使用植物生产泛醌-10的方法。提供了由植物或方法生产的含有泛醌-10的膳食补充剂，食品和食品添加剂。通过使用表达盒转化来产生植物，其中十聚丙烯酰二磷酸合成酶基因与线粒体靶向序列可操作地连接。

8. 发明授权

US5538578A Method for joining end portions of weatherstrip and apparatus for forming joining member therefor 失效
标题翻译：用于接合密封条的端部的方法和用于形成接合构件的装置
公开(公告)号：US5538578A
公开(公告)日：1996-07-23
申请号：US144283
申请日：1993-10-28
申请人： Tomio Sugawara , Hisashi Odawara , Toshikazu Kaneko , Hiroaki Shimada , Shouji Inoue
发明人： Tomio Sugawara , Hisashi Odawara , Toshikazu Kaneko , Hiroaki Shimada , Shouji Inoue
IPC分类号： B29C45/14 , B29C65/70 , B60J10/00 , B60J10/08 , B29C33/42 , B29C45/64 , F16J15/14
CPC分类号： B29C65/70 , B29C45/14467 , B29C66/1142 , B29C66/5241 , B29C66/727 , B60J10/16 , B60J10/17 , B60J10/22 , B60J10/24 , B60J10/80 , B29L2031/26 , Y10S425/127
摘要： A method of joining end portions of a weatherstrip consists of introducing the end portions to openings in a mold such that a seal portion of the weatherstrip is compressed substantially to the shape of a seal lip of the seal portion. The end portions are separated in the mold by a predetermined gap and a rubber material is injected into the gap for mutually bonding the end portions of the weatherstrip. Such a method of joining allows the weatherstrip to be installed on complexly shaped openings with no degradation of sealing characteristics over the perimeter covered by the weatherstrip. A molding apparatus for joining said end portions includes a clamping member for assuring correct positioning of a sealing edge of the weatherstrip.
摘要翻译：密封条的端部的方法包括将端部引入到模具中的开口，使得密封条的密封部分基本上被压缩到密封部分的密封唇的形状。端部在模具中以预定的间隙分开，橡胶材料被注入到间隙中，以相互粘合耐候条带的端部。这种接合方法允许风挡条安装在复杂形状的开口上，而不会在密封条所覆盖的周边上的密封特性降低。用于连接所述端部的成型装置包括用于确保密封条的密封边缘的正确定位的夹紧构件。

9. 发明授权

US4211095A Stock restraining effect adjusting mechanism of a stock guide for use with a rolling mill 失效
标题翻译：用于轧机的库存导向件的库存抑制效果调节机构
公开(公告)号：US4211095A
公开(公告)日：1980-07-08
申请号：US1887
申请日：1979-01-08
申请人： Hiroaki Shimada
发明人： Hiroaki Shimada
IPC分类号： B21B39/16
CPC分类号： B21B39/165
摘要： In a roller type stock guide of the type including, among others, a cylindrical nut member having threaded connections of opposite hands with a pair of threaded shafts and, being arranged between the two roller holder levers, operable to turn the latter in opposite directions thereby to vary the distance between the guide rollers, a ratchet type nut driving mechanism is provided which includes a ratchet wheel formed around the cylindrical nut integrally therewith, a pair of ratchet pawls movable into and out of meshing engagement with the ratchet wheel in alternate fashion under the action of a manually operable control cam, and a notching lever handle manually operable to turn the ratchet wheel and cylindrical nut in an intermittent fashion through the intermediary of either one of the ratchet pawls as placed in mesh with the ratchet wheel.
摘要翻译：在这种类型的辊式导向器中，其中包括具有相对的手与一对螺纹轴的螺纹连接的圆柱形螺母构件，并且布置在两个辊保持杆之间，可操作以使其相反的方向转动，为了改变导辊之间的距离，提供了一种棘轮式螺母驱动机构，其包括与其一体形成在圆筒形螺母周围的棘轮，一对棘爪，可以交替地移动进入和退出与棘轮啮合的棘爪手动操作的控制凸轮的动作以及手动操作的切口杆把手以棘轮方式转动棘轮和圆柱形螺母，通过与棘轮啮合的棘爪中的任意一个。

10. 发明授权

US07723569B2 Method for producing ubiquinone-10 in plant 失效
标题翻译：植物中泛醌-10的生产方法
公开(公告)号：US07723569B2
公开(公告)日：2010-05-25
申请号：US11119351
申请日：2005-04-29
申请人： Koichi Kadowaki , Sakiko Takahashi , Makoto Kawamukai , Hiroaki Shimada
发明人： Koichi Kadowaki , Sakiko Takahashi , Makoto Kawamukai , Hiroaki Shimada
IPC分类号： C12N15/82 , C12N15/11 , C12N15/31 , A01H5/00 , A01H5/10
CPC分类号： C12N9/1085 , C12N15/8243
摘要： A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.
摘要翻译：提供了表达大量泛醌-10的植物和使用植物生产泛醌-10的方法。提供了由植物或方法生产的含有泛醌-10的膳食补充剂，食品和食品添加剂。通过使用表达盒转化来产生植物，其中十聚丙烯酰二磷酸合成酶基因与线粒体靶向序列可操作地连接。

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