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1. 发明授权

US5489520A Process of producing fertile transgenic zea mays plants and progeny comprising a gene encoding phosphinothricin acetyl transferase 失效
标题翻译：生产可育转基因玉米植物和后代的方法，其包含编码膦丝菌素乙酰转移酶的基因
公开(公告)号：US5489520A
公开(公告)日：1996-02-06
申请号：US233067
申请日：1994-04-26
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

2. 发明授权

US5919675A Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US5919675A
公开(公告)日：1999-07-06
申请号：US446933
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.
摘要翻译：本发明涉及一种用于生产稳定的转基因玉米植物的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述植物中的次氯氰菊酯抗性。该方法还包括用第二基因转化所述玉米植物，并且还进一步其中所述第二基因赋予昆虫抗性，例如苏云金芽孢杆菌毒素基因的表达。实现稳定转化的方法包括组织培养方法和培养基，用所述DNA轰击受体细胞的方法，以及从转化细胞再生可育植物的方法。本发明还涉及用非转基因植物或转基因植物育种所述转基因玉米植物的方法，以及获得可育转基因植物和种子。

3. 发明授权

US5874265A Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的可育单子叶植物及其细胞的方法和组合物
公开(公告)号：US5874265A
公开(公告)日：1999-02-23
申请号：US446931
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a method for the production of stable, genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the method include, but are not limited to, wheat, barley and oats. The invention also pertains to a method of breeding said transgenic cereal plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.
摘要翻译：本发明涉及一种生产稳定的基因转化谷物植物（玉米或水稻以外）的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述谷类植物中的次氯氰菊酯抗性。在该方法范围内的谷类植物包括但不限于小麦，大麦和燕麦。本发明还涉及用非转基因植物或转基因植物育种所述转基因谷物植物的方法，以及获得可育转基因植物和种子。

4. 发明授权

US5550318A Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
公开(公告)号：US5550318A
公开(公告)日：1996-08-27
申请号：US565844
申请日：1990-08-09
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

5. 发明授权

US5969213A Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的可育单子叶植物及其细胞的方法和组合物
公开(公告)号：US5969213A
公开(公告)日：1999-10-19
申请号：US446930
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , May L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , May L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to stable and genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the invention include, but are not limited to, wheat, barley and oats. The invention also pertains to transgenic cereal plants which further comprise screenable markers and transgenic seed obtained from said plants.
摘要翻译：本发明涉及除了玉米或水稻之外的稳定和遗传转化的谷类植物，其包含表达膦丝菌素乙酰转移酶的外源DNA，以在所述谷类植物中赋予对于草丁膦的抗性。本发明范围内的谷类植物包括但不限于小麦，大麦和燕麦。本发明还涉及进一步包含从所述植物获得的可筛选标记和转基因种子的转基因谷物植物。

6. 发明授权

US07705215B1 Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
IPC分类号： C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10 , C12N15/53 , C12P7/64
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

7. 发明授权

US5134074A Embryogenic callus and cell suspensions of corn inbred B73 失效
标题翻译：玉米自交B73的胚胎愈伤组织和细胞悬浮液
公开(公告)号：US5134074A
公开(公告)日：1992-07-28
申请号：US500231
申请日：1990-03-23
申请人： Phillip N. Gordon , Thomas B. Rice
发明人： Phillip N. Gordon , Thomas B. Rice
IPC分类号： C12N5/04
CPC分类号： C12N5/04
摘要： Embryogenic callus of corn inbred B73 is disclosed along with viable, seed-producing plants derived from this embryogenic callus. Also disclosed are plantlets from embryogenic cell suspension cultures of B73 and mutants and recombinant progeny of embryogenic callus.
摘要翻译：公开了玉米近交B73的胚发生愈伤组织以及源自这种胚发生愈伤组织的活的种子生产植物。还公开了从B73的胚发生细胞悬浮培养物和胚发生愈伤组织的突变体和重组子代的小植株。

8. 发明授权

US4943978A Digital interface unit 失效
标题翻译：数字接口单元
公开(公告)号：US4943978A
公开(公告)日：1990-07-24
申请号：US298293
申请日：1989-01-17
申请人： Thomas B. Rice
发明人： Thomas B. Rice
IPC分类号： H04L29/06
CPC分类号： H04L29/06 , H04L69/08
摘要： An interface apparatus and method is disclosed which allows communication of digital data messages between a first type of data terminals (10 and 65) employing a message protocol (FIG. 1a) with a "free text-type" message, and other user devices employing other digital message protocols. The invention is adapted to exploit the free text message capability of the first protocol. In accordance with the invention, the incoming user data messages are packed into one or more free text messages of the first message protocol, and conversely "unpack" free text messages from the data terminal employing the first protocol are unpacked and formatted into the message protocol format employed by the user device 65. The invention eliminates the necessity for expensive translation devices which translate the data, and allows a plurality of user data terminals to communicate via a link comprising the data terminals employing the first message protocol.
摘要翻译：公开了一种接口装置和方法，其允许在采用具有“自由文本类型”消息的消息协议（图1a）的第一类型的数据终端（10和65）之间进行数字数据消息的通信，以及采用其他数字消息协议。本发明适用于利用第一协议的自由文本消息能力。根据本发明，输入的用户数据消息被打包成第一消息协议的一个或多个自由文本消息，并且相反地，使用第一协议的数据终端“解包”自由文本消息被解包并格式化成消息协议本发明消除了转换数据的昂贵的翻译装置的必要性，并且允许多个用户数据终端经由包含采用第一消息协议的数据终端的链路进行通信。

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