专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20230099373A1 METHOD OF DESIGNING CARBOHYDRATES 有权
公开(公告)号：US20230099373A1
公开(公告)日：2023-03-30
申请号：US17777922
申请日：2020-11-19
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： Nathan E. Lewis , Wan-Tien Chiang , Chenguang Liang
IPC分类号： C12N15/10
摘要： Glycosylated biopharmaceuticals are important in the global pharmaceutical market. Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute. To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend an approach to model biosynthetic pathways for all measured glycans, and the Markov chain modeling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout. We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells, along with o-glycosylation and lipid profiles. Our models accurately predict N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases. By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics. We further apply this to study differential O-glycosylation and lipidomics. Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases and other enzyme classes, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation and the lipidome.

2. 发明申请

US20220356488A1 Asparaginase Based Selection System for Heterologous Protein Expression in Mammalian Cells 有权
公开(公告)号：US20220356488A1
公开(公告)日：2022-11-10
申请号：US17738896
申请日：2022-05-06
申请人： The Regents of the University of California , Technical University of Denmark
发明人： Hooman Hefzi , Nathan E. Lewis , Karen Julie la Cour Karottki , Helene Faustrup Kildegaard
IPC分类号： C12N15/85 , C12N9/82 , C12N9/00 , C12N15/52 , C12P21/00 , C12N15/90 , C07K14/705
摘要： A non-naturally occurring cell comprising an inoperative genomic asparaginase (Aspg) gene and an inoperative glutamine synthetase (Gs) gene, wherein the cell has been transfected with a controllably expressed gene encoding an enzyme having asparaginase activity, a controllably expressed gene encoding an enzyme having glutamine synthetase activity, and a controllably expressed gene encoding a heterologous protein of interest.

3. 发明授权

US11242510B2 Mammalian cells devoid of lactate dehydrogenase activity 有权
公开(公告)号：US11242510B2
公开(公告)日：2022-02-08
申请号：US16096784
申请日：2017-05-01
申请人： The Regents of the University of California
发明人： Hooman Hefzi , Nathan E. Lewis
IPC分类号： C12N9/04 , A61K39/395 , A61K35/54 , C12N5/00 , C12N5/071 , C12N9/12 , C12N15/90 , C12P21/00
摘要： Provided are mammalian cells devoid of lactate dehydrogenase activity.

4. 发明公开

US20240355424A1 CLINICAL DIAGNOSTICS USING GLYCANS 审中-公开
公开(公告)号：US20240355424A1
公开(公告)日：2024-10-24
申请号：US18688235
申请日：2022-08-31
申请人： The Regents of the University of California
发明人： Nathan E. Lewis , Wan Tien Chiang , Benjamin P. Kellman , Bokan Bao , Matthew Songmin Schinn
IPC分类号： G16B40/10 , G01N30/02 , G01N30/72 , G01N30/88 , G16H50/20
CPC分类号： G16B40/10 , G01N30/7233 , G01N30/88 , G16H50/20 , G01N2030/027 , G01N2030/8813
摘要： Method for using glycan or glycomics data for classification, diagnosis, prognosis, subject stratification, or therapy decision making based on a sample with glycans or glycosylated molecules.

5. 发明申请

US20190256824A1 MAMMALIAN CELLS DEVOID OF LACTATE DEHYDROGENASE ACTIVITY 审中-公开
公开(公告)号：US20190256824A1
公开(公告)日：2019-08-22
申请号：US16096784
申请日：2017-05-01
申请人： The Regents of the University of California
发明人： Hooman Hefzi , Nathan E. Lewis
IPC分类号： C12N9/04 , C12N9/12 , C12N15/90 , C12N5/071 , C12P21/00
摘要： Provided are mammalian cells devoid of lactate dehydrogenase activity.

6. 发明公开

US20240093259A1 METHODS TO STABILIZE MAMMALIAN CELLS 审中-公开
公开(公告)号：US20240093259A1
公开(公告)日：2024-03-21
申请号：US17767844
申请日：2020-10-09
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： Nathan E. Lewis , Philipp Spahn , Shangzhong Li , Hooman Hefzi , Isaac Shamie
IPC分类号： C12P21/00 , C12N15/86 , C12Q1/6897
CPC分类号： C12P21/00 , C12N15/86 , C12Q1/6897 , C12N2740/15043
摘要： The invention provides gene targets whose restoration leads to genome stabilization in host cells, such as Chinese Hamster Ovary (CHO) cells. Many DNA repair genes are mutated in CHO cells which compromises their ability to repair naturally occurring DNA damage, in particular double-strand breaks (DSBs). Unrepaired DSBs can give rise to chromosomal instability which, in turn, can lead to loss of transgenes from the genome. As a consequence, protein titer can drop significantly, rendering protein production unprofitable. The invention provides a set of mutated DNA repair genes whose restoration yields significant improvement in DSB repair, genome stability, and protein titer.

7. 发明授权

US11670399B2 Systems and methods for predicting glycosylation on proteins 有权
公开(公告)号：US11670399B2
公开(公告)日：2023-06-06
申请号：US15573239
申请日：2016-05-18
申请人： The Regents of the University of California
发明人： Philipp N. Spahn , Nathan E. Lewis
IPC分类号： G01N33/48 , G16B40/00 , G01N33/68 , G01N33/50 , C12P21/00 , G16C20/60 , G16B5/20 , G16B35/10 , G16H50/50 , G06N7/08
CPC分类号： G16B40/00 , C12P21/005 , G01N33/50 , G01N33/68 , G06N7/08 , G16B5/20 , G16B35/10 , G16C20/60 , G16H50/50 , Y02A90/10
摘要： The disclosed technology provides a computational prediction modeling comprising a novel algorithm for prediction of glycosylation or to optimize biopharmaceutical production of proteins of therapeutic relevance. The model of the disclosed technology can be used to predict glycosylation changes based solely on the stating glycoprofiles in any host cells and known or suggested rules on enzyme specificity. Applications of the invention model are also provided.

8. 发明申请

US20180101643A1 Systems and Methods for Predicting Glycosylation on Proteins 审中-公开
公开(公告)号：US20180101643A1
公开(公告)日：2018-04-12
申请号：US15573239
申请日：2016-05-18
申请人： The Regents of the University of California
发明人： Philipp N. Spahn , Nathan E. Lewis
IPC分类号： G06F19/24 , G06N7/08 , G16H50/50
CPC分类号： G16B40/00 , C12P21/005 , G01N33/50 , G01N33/68 , G06N7/08 , G16B5/20 , G16C20/60 , G16H50/50 , Y02A90/26
摘要： The disclosed technology provides a computational prediction modeling comprising a novel algorithm for prediction of glycosylation or to optimize biopharmaceutical production of proteins of therapeutic relevance. The model of the disclosed technology can be used to predict glycosylation changes based solely on the stating glycoprofiles in any host cells and known or suggested rules on enzyme specificity. Applications of the invention model are also provided.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式