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1. 发明授权

US09040257B2 Basidiomycetous yeast mutant 有权
标题翻译： Basidiomycetous酵母突变体
公开(公告)号：US09040257B2
公开(公告)日：2015-05-26
申请号：US14394691
申请日：2013-04-23
申请人： TOYOBO CO., LTD. , NATIONAL RESEARCH INSTITUTE OF BREWING
发明人： Yuu Utashima , Takahide Kishimoto , Shusaku Yanagidani , Kazuo Masaki
IPC分类号： C12P21/02 , C12N1/16 , C12R1/645 , C12P21/00
CPC分类号： C12N1/16 , C12P21/00 , C12P21/02 , C12R1/645
摘要： The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.
摘要翻译：本发明提供了一种用于基因重组的宿主，其用于大规模生产异源蛋白质，通过抑制担子酵母酵母中细胞外多糖的产生。隐球菌属 S-2D11菌株（FERM BP-11482），其特征在于与亲本菌株相比抑制细胞外多糖的产生。

2. 发明申请

US20150072379A1 BASIDIOMYCETOUS YEAST MUTANT 有权
标题翻译：。。。。。
公开(公告)号：US20150072379A1
公开(公告)日：2015-03-12
申请号：US14394691
申请日：2013-04-23
申请人： TOYOBO CO., LTD. , NATIONAL RESEARCH INSTITUTE OF BREWING
发明人： Yuu Utashima , Takahide Kishimoto , Shusaku Yanagidani , Kazuo Masaki
IPC分类号： C12N1/16 , C12P21/00 , C12R1/645
CPC分类号： C12N1/16 , C12P21/00 , C12P21/02 , C12R1/645
摘要： The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERN BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.
摘要翻译：本发明提供了一种用于基因重组的宿主，其用于大规模生产异源蛋白质，通过抑制担子酵母酵母中细胞外多糖的产生。隐球菌属 S-2D11菌株（FERN BP-11482），其特征在于与亲本菌株相比抑制细胞外多糖的产生。

3. 发明授权

US11591631B2 Glucose monitoring method and glucose sensor 有权
公开(公告)号：US11591631B2
公开(公告)日：2023-02-28
申请号：US16629559
申请日：2018-06-22
申请人： TOYOBO CO., LTD.
发明人： Yasuhiro Yamazaki , Atsushi Kawai , Takahide Kishimoto
IPC分类号： C12Q1/32 , C12Q1/00 , C12N9/04
摘要： A glucose monitoring method and a glucose sensor, both of which use glucose dehydrogenase having a Michaelis constant (Km) for xylose of 600 mM or more and 3000 mM or less, and a Km for glucose of 0.1 mM or more and 100 mM or less, which provide for evaluating FADGDH in an aqueous system while reducing the practical influence of FADGDH on D-xylose.

4. 发明授权

US09404144B2 Glucose dehydrogenase 有权
标题翻译：葡萄糖脱氢酶
公开(公告)号：US09404144B2
公开(公告)日：2016-08-02
申请号：US14374164
申请日：2013-02-07
申请人： TOYOBO CO., LTD.
发明人： Yosuke Sumida , Rie Hirao , Yuu Utashima , Hiroshi Kawaminami , Hiroshi Aiba , Takahide Kishimoto , Shusaku Yanagidani
IPC分类号： C12N9/04 , C12N15/53 , C12Q1/32 , C12Q1/54
CPC分类号： C12Q1/54 , C12N9/0006 , C12Q1/32 , C12Y101/01047 , C12Y101/05 , G01N2333/904
摘要： An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.
摘要翻译：本发明的目的是提供一种新的葡萄糖脱氢酶，葡萄糖脱氢酶的生产方法和葡萄糖脱氢酶的应用。本发明的黄素结合葡萄糖脱氢酶具有以下特征（1）和（4）：（1）分子量：通过SDS-聚丙烯酰胺电泳测定，酶中多肽部分的分子量为约68kDa; （2）Km值：D-葡萄糖的Km值为约15mM以下; （3）温度稳定性：在55℃以下的温度下稳定; 和（4）pH稳定性：在3.0至8.5的pH范围内是稳定的。

5. 发明申请

US20140349327A1 METHOD FOR PRODUCING FRUCTOSYL VALYL HISTIDINE OXIDASE PREPARATION 审中-公开
标题翻译：生产木瓜胆固醇氧化酶制剂的方法
公开(公告)号：US20140349327A1
公开(公告)日：2014-11-27
申请号：US14371288
申请日：2013-01-10
申请人： TOYOBO CO., LTD.
发明人： Rie Hirao , Takahide Kishimoto , Shusaku Yanagidani
IPC分类号： C12N9/06 , C12Q1/26
CPC分类号： C12N9/0032 , C12N9/0022 , C12Q1/26 , G01N33/723
摘要： ObjectAn object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation.Means for achieving the objectA method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.
摘要翻译：发明目的本发明的目的是提供高度稳定的FVHO制剂和低吸湿性干燥的FVHO制剂。用于实现目的的方法一种用于制备FVHO制剂的方法，包括以下步骤：允许至少一种选自磷酸，酪蛋白胨，D-葡糖胺盐酸盐，蜜二糖，山梨糖，乳糖，果糖，松三糖，葡萄糖酸-1,5- 内酯和核糖醇; 以及用于制备干燥的FVHO制剂的方法，包括允许Bicine共存的步骤。

6. 发明授权

US09260699B2 Glucose dehydrogenase 有权
标题翻译：葡萄糖脱氢酶
公开(公告)号：US09260699B2
公开(公告)日：2016-02-16
申请号：US14266304
申请日：2014-04-30
申请人： TOYOBO CO., LTD.
发明人： Yosuke Sumida , Hiroshi Aiba , Hiroshi Kawaminami , Rie Hirao , Yuu Utashima , Takahide Kishimoto , Shusaku Yanagidani
IPC分类号： C12N9/04 , C12Q1/00 , C12Q1/32
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32
摘要： An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.
摘要翻译：本发明的目的是提供一种新的葡萄糖脱氢酶，葡萄糖脱氢酶的生产方法和葡萄糖脱氢酶的应用。提供了包含具有与SEQ ID NO：1的氨基酸序列具有80％或更高同一性并具有葡萄糖脱氢酶活性的氨基酸序列的多肽的分离的黄素结合性葡萄糖脱氢酶。

7. 发明授权

US09133446B2 Alkaline phosphatase 有权
标题翻译：碱性磷酸酶
公开(公告)号：US09133446B2
公开(公告)日：2015-09-15
申请号：US13973557
申请日：2013-08-22
申请人： TOYOBO CO., LTD.
发明人： Hiroshi Aiba , Takahide Kishimoto , Yoshiaki Nishiya
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , C12Y301/03001
摘要： The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus Shewanella and has the following characteristics: (A) molecular weight: about 104,000; (B) optimum reaction pH: about 9.5; (C) stable pH range: 5.5 to 10.4; (D) thermal stability: 65° C., and (E) specific activity: 5,000 U/mg or more.
摘要翻译：本发明提供具有高比活性的AP，并且优选相对于通常用于高灵敏度免疫测定分析的各种发光底物具有优异的反应性。进一步优选地，本发明提供具有比CIAP高的热稳定性的AP。本发明的碱性磷酸酶衍生自希瓦氏菌属，具有以下特征：（A）分子量：约104,000; （B）最佳反应pH：约9.5; （C）稳定pH范围：5.5〜10.4; （D）热稳定性：65℃，（E）比活度：5000U / mg以上。

8. 发明申请

US20150031059A1 NOVEL GLUCOSE DEHYDROGENASE 有权
标题翻译：新葡萄糖脱氢酶
公开(公告)号：US20150031059A1
公开(公告)日：2015-01-29
申请号：US14374164
申请日：2013-02-07
申请人： TOYOBO CO., LTD.
发明人： Yosuke Sumida , Rie Hirao , Yuu Utashima , Hiroshi Kawaminami , Hiroshi Aiba , Takahide Kishimoto , Shusaku Yanagidani
IPC分类号： C12Q1/54 , C12N9/04
CPC分类号： C12Q1/54 , C12N9/0006 , C12Q1/32 , C12Y101/01047 , C12Y101/05 , G01N2333/904
摘要： An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.
摘要翻译：本发明的目的是提供一种新的葡萄糖脱氢酶，葡萄糖脱氢酶的生产方法和葡萄糖脱氢酶的应用。本发明的黄素结合葡萄糖脱氢酶具有以下特征（1）和（4）：（1）分子量：通过SDS-聚丙烯酰胺电泳测定，酶中多肽部分的分子量为约68kDa; （2）Km值：D-葡萄糖的Km值为约15mM以下; （3）温度稳定性：在55℃以下的温度下稳定; 和（4）pH稳定性：在3.0至8.5的pH范围内是稳定的。

9. 发明授权

US11767349B2 Modified streptolysin O 有权
公开(公告)号：US11767349B2
公开(公告)日：2023-09-26
申请号：US16304962
申请日：2017-05-26
申请人： TOYOBO CO., LTD.
发明人： Yosuke Sumida , Hiroaki Kitazawa , Takahide Kishimoto
IPC分类号： C07K14/315 , C07K16/12 , G01N33/68
CPC分类号： C07K14/315 , C07K16/1275 , G01N33/6854
摘要： The invention provides streptolysin O containing a polypeptide comprising an amino acid sequence having deletion of a polypeptide extending from a lysine residue at position 2 of SEQ ID NO: 1 to any of an alanine residue at position 31, a glutamate residue at position 32, a serine residue at position 33, and an asparagine residue at position 34, and also having deletion of any of an isoleucine residue at position 465 to an alanine residue at position 472 of SEQ ID NO: 1 and all of the subsequent amino acid residues, as well as variants thereof and related DNA, vectors, transformants, and methods of use thereof.

10. 发明授权

US09994827B2 Method for producing fructosyl valyl histidine oxidase preparation 有权
公开(公告)号：US09994827B2
公开(公告)日：2018-06-12
申请号：US14371288
申请日：2013-01-10
申请人： TOYOBO CO., LTD.
发明人： Rie Hirao , Takahide Kishimoto , Shusaku Yanagidani
IPC分类号： C12N9/06 , C12Q1/26 , G01N33/72
CPC分类号： C12N9/0032 , C12N9/0022 , C12Q1/26 , G01N33/723
摘要： ObjectAn object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation.Means for achieving the objectA method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.

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