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1. 发明授权

US06465634B1 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 失效
公开(公告)号：US06465634B1
公开(公告)日：2002-10-15
申请号：US09415900
申请日：1999-10-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
IPC分类号： C07H2104
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

2. 发明授权

US06391632B1 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 有权
标题翻译：基于重组甲病毒的载体具有抑制细胞大分子合成的作用
公开(公告)号：US06391632B1
公开(公告)日：2002-05-21
申请号：US09415784
申请日：1999-10-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
IPC分类号： C12N510
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as is compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
摘要翻译：公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平类型，以及与病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质，与野生型重组甲病毒颗粒相比。还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

3. 发明授权

US06451592B1 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 失效
公开(公告)号：US06451592B1
公开(公告)日：2002-09-17
申请号：US08944465
申请日：1997-10-06
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
IPC分类号： C12N1500
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

4. 发明授权

US07811812B2 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 失效
标题翻译：基于重组甲病毒的载体具有抑制细胞大分子合成的作用
公开(公告)号：US07811812B2
公开(公告)日：2010-10-12
申请号：US10391441
申请日：2003-03-17
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Drvga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Drvga , Ilya Frolov
IPC分类号： C12N15/63
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
摘要翻译：公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

5. 发明授权

US06592874B2 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 失效
公开(公告)号：US06592874B2
公开(公告)日：2003-07-15
申请号：US09507362
申请日：2000-02-18
申请人： Sondra Schlesinger , Ilya Frolov
发明人： Sondra Schlesinger , Ilya Frolov
IPC分类号： A61K39193
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

6. 发明授权

US06426196B1 Alphavirus structural protein expression cassettes 有权
标题翻译：甲病毒结构蛋白表达盒
公开(公告)号：US06426196B1
公开(公告)日：2002-07-30
申请号：US09415785
申请日：1999-10-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Sondra Schlesinger , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Sondra Schlesinger , Ilya Frolov
IPC分类号： C12P2106
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed. comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
摘要翻译：公开了分离的核酸分子。包括甲型病毒非结构蛋白基因，当与野生型相比，当可操作地并入重组甲病毒颗粒中时，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平，并且相同或与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的更高水平的蛋白质。还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

7. 发明授权

US6136538A Silent inducible virus replicons and uses thereof 有权
标题翻译：沉默诱导型病毒复制子及其用途
公开(公告)号：US6136538A
公开(公告)日：2000-10-24
申请号：US227556
申请日：1999-01-08
申请人： Paul D. Olivo , Sondra Schlesinger
发明人： Paul D. Olivo , Sondra Schlesinger
IPC分类号： C12N5/10 , C12N15/40 , C12N15/85 , C12N15/86 , C12Q1/68 , C12Q1/70 , C07H21/04 , A61K48/00
CPC分类号： C12N7/00 , C12N15/85 , C12N15/86 , C12Q1/6897 , C12Q1/70 , C12Q1/702 , C12N2770/36143 , C12N2770/36152 , C12N2830/00 , C12N2830/60 , C12N2840/20 , C12N2840/203
摘要： A recombinant cell stably transformed with a cDNA of a silent, inducible replicon encoding a recombinant protein is disclosed. Transcription of the replicon cDNA is under the control of a silent promoter inducible by a DNA virus and expression of the recombinant protein is dependent upon the presence of the DNA virus in the cell. The cell can be engineered to package the replicon upon infection by the DNA virus, leading to intercellular amplification of expression of the recombinant protein. Where the recombinant protein is a reporter gene product, the recombinant cell may be used in an assay for detecting DNA viruses. A kit for performing such an assay is also provided.
摘要翻译：公开了用编码重组蛋白的沉默的诱导型复制子的cDNA稳定转化的重组细胞。复制子cDNA的转录受DNA病毒诱导的沉默启动子的控制，重组蛋白的表达取决于DNA病毒在细胞中的存在。该细胞可被设计成在DNA病毒感染时包装复制子，导致重组蛋白质的表达的细胞间扩增。当重组蛋白是报告基因产物时，重组细胞可用于检测DNA病毒的测定中。还提供了用于进行这种测定的试剂盒。

8. 发明授权

US06458560B1 Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis 失效
标题翻译：基于重组甲病毒的载体具有抑制细胞大分子合成的作用
公开(公告)号：US06458560B1
公开(公告)日：2002-10-01
申请号：US09415868
申请日：1999-10-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilva Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilva Frolov
IPC分类号： C12P2106
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
摘要翻译：公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

9. 发明授权

US06270958B1 Detection of negative-strand RNA viruses 有权
标题翻译：检测负链RNA病毒
公开(公告)号：US06270958B1
公开(公告)日：2001-08-07
申请号：US09253445
申请日：1999-02-19
申请人： Paul D. Olivo , Sondra Schlesinger , Mark E. Peeples , Peter Collins
发明人： Paul D. Olivo , Sondra Schlesinger , Mark E. Peeples , Peter Collins
IPC分类号： C12Q170
CPC分类号： C12Q1/6897 , C12N2503/00 , C12N2510/00 , Y10S435/81 , Y10S435/975
摘要： A diagnostic assay for detecting a negative-strand RNA virus in a sample and a genetically engineered cell for use in the assay are disclosed. The cell expresses a heterologous DNA-dependent RNA polymerase that synthesizes a minigenome or miniantigenome of the RNA virus from a cDNA template present in the cell. The cell also expresses the nucleocapsid proteins of the negative-strand virus that are necessary for replication of the minigenome or miniantigenome. Infection of the cell by the negative-strand virus results in expression of a reporter gene product encoded by the miniantigenome.
摘要翻译：公开了用于检测样品中的负链RNA病毒的诊断测定法和用于测定中的遗传工程细胞。细胞表达异源DNA依赖性RNA聚合酶，其从存在于细胞中的cDNA模板合成RNA病毒的小基因组或微型基因组。细胞还表达了负链病毒的核衣壳蛋白，其是复制小基因组或微型基因组所必需的。通过负链病毒感染细胞导致由本发明组编码的报告基因产物的表达。

10. 发明授权

US5591579A Indicator cell line for detecting RNA viruses and method therefor 失效
标题翻译：用于检测RNA病毒的指示细胞系及其方法
公开(公告)号：US5591579A
公开(公告)日：1997-01-07
申请号：US171214
申请日：1993-12-21
申请人： Paul D. Olivo , Sondra Schlesinger
发明人： Paul D. Olivo , Sondra Schlesinger
IPC分类号： C07K14/08 , C12N5/10 , C12N15/09 , C12Q1/34 , C12Q1/66 , C12Q1/68 , C12Q1/70 , G01N33/569 , C12N5/16 , C12N7/00 , C12N15/00
CPC分类号： C07K14/005 , C12Q1/6897 , G01N33/56983 , C12N2760/18822 , G01N2333/085 , G01N2333/18 , Y10S435/975
摘要： Cell lines and methods are disclosed for detecting the presence of RNA viruses in a specimen. The cell lines are stably transformed with a DNA molecule that includes a promoter capable of being recognized by the DNA dependent RNA polymerase of the cell capable of directing the transcription of a cDNA of a structurally defective RNA virus genome operably coupled to the promoter. The cDNA contains a structural coding sequence encoding a selected reporter gene product. The RNA molecules transcribed by the DNA dependent RNA polymerase are not capable of causing the translation of the reporter gene in the cell except when an active related virus that provides the necessary trans-acting enzymes to cause the increased replication of the RNA containing the reporter gene which is then translated into the reporter gene product is provided. Methods utilizing the cell lines of this invention to detect RNA viruses in a specimen by incubating the specimen with the cell line and assaying for expression of the reporter gene and a kit containing a supply of the cells and a supply of the reagents necessary for the detection of the reporter gene product are also provided.
摘要翻译：公开了用于检测样品中RNA病毒的存在的细胞系和方法。细胞系被包括能够被能够引导结构有缺陷的RNA病毒基因的cDNA的转录的细胞的DNA依赖性RNA聚合酶识别的启动子的DNA分子稳定转化，所述基因可操作地连接到启动子上。 cDNA含有编码所选报道基因产物的结构编码序列。由DNA依赖性RNA聚合酶转录的RNA分子不能引起报告基因在细胞中的翻译，除了当提供必需的反式作用酶以引起含有报告基因的RNA的复制增加的活性相关病毒时然后将其翻译成报告基因产物。利用本发明的细胞系的方法通过将样品与细胞系一起孵育并测定报告基因的表达和含有细胞供应的试剂盒和检测所必需的试剂的供应来检测标本中的RNA病毒的报告基因产物也被提供。

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