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    • 2. 发明申请
    • DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme
    • 用于抑制产生Lachrymatory因子的酶基因表达的DNA和载体,用它们抑制产生lachrymative因子的酶基因表达的方法和产生产生Lachrymatory因子的酶的基因的表达的蔬菜
    • US20050022263A1
    • 2005-01-27
    • US10932950
    • 2004-09-01
    • Shinsuke ImaiNobuaki TsugeYasuhiro KamataNoriya MasamuraJinji ShonoKentaro Horie
    • Shinsuke ImaiNobuaki TsugeYasuhiro KamataNoriya MasamuraJinji ShonoKentaro Horie
    • C12N9/90C12N15/29C12N15/82A01H1/00C07H21/04C12N5/04C12N9/02
    • C12N15/8243C12N9/90C12N15/8242
    • The object of the present invention is to provide DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable. The present invention relates to DNA comprising at least one sequence selected from the following sequences and a regulatory sequence connected to said sequence so as to make the transcription possible: (a) a DNA sequence encoding a protein or a polypeptide of a lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations, wherein the protein or the polypeptide has an effect of converting 1-propenylsulfenic acid into the lachrymatory factor; (b) a regulatory sequence of a DNA in a vegetable genome determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the regulatory sequence in a sense orientation, an antisense orientation or both the orientations; and (c) a DNA sequence located between the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme and the regulatory sequence of the DNA in the vegetable genome DNA determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations.
    • 本发明的目的是提供基于酶基因序列设计的DNA和RNA,其用于抑制来自产生泪液因子的前体的产生泪液因子的酶基因的表达,以及引入所需的载体 用于将产生致命菌素的酶的基因的表达抑制成植物的DNA。 本发明涉及包含至少一个选自以下序列的序列的DNA,以及与所述序列连接的调控序列,以使转录成为可能:(a)编码产生产生泪液的因子的蛋白质或多肽的DNA序列 酶或一部分DNA序列在正义取向,反义方向或取向两者中,其中所述蛋白质或多肽具有将1-丙烯基亚磺酸转化为变应原因子的作用; (b)基于编码产生产生泪液因子的酶的蛋白质或多肽的DNA或有义方向,反义方向或两者的一部分调节序列确定的植物基因组DNA的调节序列 方向; 和(c)位于编码产生致命菌素的酶的蛋白质或多肽的DNA与植物基因组DNA中的DNA的调节序列之间的DNA序列,其基于编码所述蛋白质或多肽的DNA 衍生因子产生酶或部分DNA序列在正向,反义方向或取向上。