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    • 1. 发明申请
    • GENE OF ENZYME HAVING ACTIVITY TO GENERATE LACHRYMATORY FACTOR
    • 具有活性的酵母基因生成LACHRYMATORY因子
    • US20090081754A1
    • 2009-03-26
    • US12051023
    • 2008-03-19
    • Shinsuke IMAINobuaki TsugeMuneaki Tomotake
    • Shinsuke IMAINobuaki TsugeMuneaki Tomotake
    • C12N9/00C12N15/52C12N15/74C07H21/02C12N1/00
    • C12N9/90C07K14/415C12N9/88
    • It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.
    • 本发明的一个目的是提供产生泪液因子的酶的同工酶,这些同工酶的氨基酸序列和编码这些氨基酸序列的基因,本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶,由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列,SEQ ID No.4所示的DNA 和5,其包含编码上述蛋白质或多肽的碱基序列,产生上述同功酶的方法,含有上述DNA的重组载体,通过用上述重组载体转化宿主细胞形成的转化体,制备 蛋白质或多肽,其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。