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    • 6. 发明授权
    • Mold and manufacturing method thereof
    • 模具及其制造方法
    • US09005502B2
    • 2015-04-14
    • US13405996
    • 2012-02-27
    • Tsuyoshi ChibaHiroshi FujitaYuki Aritsuka
    • Tsuyoshi ChibaHiroshi FujitaYuki Aritsuka
    • B29C33/40B29C33/64B82Y10/00B29C33/42
    • B29C33/40B29C33/405B29C33/424B29C33/64B29K2883/00B82Y10/00
    • The object of the invention is to provide a mold that is capable of high-precision, stable patterning, and improved in terms of handleability as well, and a manufacturing method thereof. The mold of the invention has a pattern-formation surface defined by one surface, wherein the pattern-formation layer has a projection-and-depression structure area for patterning, and a base surface defined by another surface. At least the pattern-formation layer and the base surface each comprise a polydimethylsiloxane layer. Given a low-molecular-weight siloxane of a cyclic structure represented by [—Si(CH3)2O—]k where k is an integer of 3 to 20 inclusive, the polydimethylsiloxane layer positioned on the pattern-formation layer comprises a high-content polydimethylsiloxane layer containing the low-molecular-weight siloxane in a larger amount, and the polydimethylsiloxane layer positioned on the base surface comprises a low-content polydimethylsiloxane layer containing the low-molecular-weight siloxane in a smaller amount.
    • 本发明的目的是提供一种能够高精度,稳定地图案化并且在可操作性方面得到改进的模具及其制造方法。 本发明的模具具有由一个表面限定的图形形成表面,其中图案形成层具有用于图案化的突起 - 凹陷结构区域和由另一个表面限定的基底表面。 至少图案形成层和基底表面各自包含聚二甲基硅氧烷层。 给出由[-Si(CH3)2O-] k表示的环状结构的低分子量硅氧烷,其中k是3至20的整数,其中位于图案形成层上的聚二甲基硅氧烷层包含高含量 含有较大量的低分子量硅氧烷的聚二甲基硅氧烷层和位于基材表面上的聚二甲基硅氧烷层包含含有较少量的低分子量硅氧烷的低含量聚二甲基硅氧烷层。
    • 7. 发明授权
    • Method for assaying keratan sulfate, assay kit therefor and method for detecting joint disease by using the same
    • 用于测定硫酸角质素的方法,其测定试剂盒及其用于检测关节疾病的方法
    • US08822229B2
    • 2014-09-02
    • US13142262
    • 2009-12-24
    • Shigeyuki WakitaniHiroshi FujitaTakeshi IshimaruKoji YamamotoYasuhiro KurahashiJunichi OnayaHiroyuki Masuda
    • Shigeyuki WakitaniHiroshi FujitaTakeshi IshimaruKoji YamamotoYasuhiro KurahashiJunichi OnayaHiroyuki Masuda
    • G01N33/564
    • G01N33/53G01N2400/40G01N2800/102
    • The inventions provides a method for immunologically determining a keratan sulfate level which method includes bringing an anti-keratan sulfate monoclonal antibody into contact with a biological sample, the anti-keratan sulfate monoclonal antibody exhibiting a relative reaction specificity between keratan sulfate-I and keratan sulfate-II represented by IC50KS-I/KS-II of 0.4 to 5, to thereby provide a signal; and detecting keratan sulfate contained in the biological sample from the signal. On the basis of the method, the invention also provides a joint disease detection method and a method for assessing the effect of a remedy for a joint disease and a candidate substance therefor. Through these methods, a very small amount of keratan sulfate contained in a sample, can be determined. Particularly, these methods can determine, at high-sensitivity and high-specificity, the total keratan sulfate including keratan sulfate-I, which have been difficult to determine through a conventional technique. The methods also enables detect a joint disease and assess the effect of a remedy for a joint disease or a candidate substance therefor.
    • 本发明提供了一种免疫测定硫酸角质素水平的方法,该方法包括使硫酸角质素硫酸单克隆抗体与生物样品接触,硫酸角质素-I与硫酸角质素之间呈现相对反应特异性的硫酸角质素单体抗体 -II由IC50KS-I / KS-II表示,为0.4〜5,从而提供信号; 并从信号中检测生物样品中所含的硫酸角质素。 在该方法的基础上,本发明还提供了关于联合疾病的检测方法和评估对于关节疾病及其候选物质的补救效果的方法。 通过这些方法,可以测定样品中所含的极少量硫酸角质素。 特别地,这些方法可以高灵敏度和高特异性地确定通过常规技术难以确定的总硫酸角质素,包括硫酸角质素-I。 该方法还能够检测关节疾病并评估对于关节疾病或候选物质的补救的效果。