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1. 发明申请

US20120288893A1 SEED-SPECIFIC EXPRESSION VECTOR AND ITS CONSTRUCTION METHODS AND APPLICATIONS 有权
标题翻译：种子特异性表达载体及其构建方法和应用
公开(公告)号：US20120288893A1
公开(公告)日：2012-11-15
申请号：US13515209
申请日：2010-12-09
申请人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Lingyuan Hu , Haimin Xu , Chenggang Yu
发明人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Lingyuan Hu , Haimin Xu , Chenggang Yu
IPC分类号： C12N15/82 , C12P21/00 , C12P19/34
CPC分类号： C07K14/775 , C12N15/8257
摘要： A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.
摘要翻译：公开了种子特异性表达载体及其构建方法和应用。由植物二元表达载体pBI121的HindIII和SacI位点之间插入由油菜籽油质蛋白基因启动子驱动的花生梭菌油质蛋白基因 - 载脂蛋白A-IMilano（A-IM）基因组成的融合蛋白表达盒，获得植物表达载体本发明的pBINOA。此外，提供了一种生产载脂蛋白A-IMilano的方法，其中表达载体用于转化用作植物生物反应器的油向日葵。该方法不仅可以提高载脂蛋白A-IMilano的产量，而且大大降低生产成本，适用于工业生产。

2. 发明授权

US09745362B2 Seed-specific expression vector and its construction methods and applications 有权
公开(公告)号：US09745362B2
公开(公告)日：2017-08-29
申请号：US13515209
申请日：2010-12-09
申请人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu
发明人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu
IPC分类号： C07K14/775 , C12N15/82
CPC分类号： C07K14/775 , C12N15/8257
摘要： A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.

3. 发明申请

US20080043399A1 Method and Device of Compound Active and Passive Plasma for Lightning Protection 失效
标题翻译：复合主动和被动等离子体的防雷方法和装置
公开(公告)号：US20080043399A1
公开(公告)日：2008-02-21
申请号：US11547178
申请日：2005-01-27
申请人： Kunsheng Wang , Xiaobin Li
发明人： Kunsheng Wang , Xiaobin Li
IPC分类号： H01H47/00
CPC分类号： H02G13/00 , H02G13/20 , H02G13/60 , H02G13/80
摘要： A lightning protection method and its device with compound actively generated plasma and passively produced plasma are disclosed, in witch conventional lightning rods are reformed each with a plasma piping inside. The high density plasma generated by an active plasma generator is blown through the piping, spread out at the tips of the rods and compounded with the plasma passively produced by the corona discharge on the tips under the action of lightning cloud electric field. The compound plasma dissipates the electric charges, on the tips of the rods, inducted by lightning cloud electric field, and neutralizes the electric charges of the lightning cloud. Then the electric field strength in around space can be reduced, and the lightning cloud voltage puncture to the rods can be shielded and eliminated. As a result, the thunderstruck to the protected object within the protection scope related to the lightning rods of the device can be rejected. According to the method, the lightning rods of the device do not need to be grounded for lightning protection, but it is compatible to ground each lightning rod via a gap. The device of the invention is practical, economic and reliable.
摘要翻译：公开了一种具有复合主动产生的等离子体和被动产生的等离子体的防雷方法及其装置，其中传统的避雷针各自用等离子体管道内部重整。由活性等离子体发生器产生的高密度等离子体通过管道吹出，在棒的尖端处展开，并与雷电云电场作用下由尖端上的电晕放电被动产生的等离子体混合。复合等离子体消散由雷电云电场引起的杆尖，并消除了雷电的电荷。然后可以减小周围的电场强度，并可以屏蔽和消除雷击云电压对杆的刺穿。因此，与设备的避雷针有关的保护范围内的受保护物体的雷击可以被拒绝。根据该方法，设备的避雷针不需要接地用于防雷，而是通过间隙对每个避雷针进行接地。本发明的装置实用，经济可靠。

4. 发明申请

US20180226782A1 PASSIVE COMPOUND STRONG-IONIZATION DISCHARGING PLASMA LIGHTNING REJECTION DEVICE 审中-公开
公开(公告)号：US20180226782A1
公开(公告)日：2018-08-09
申请号：US15737570
申请日：2015-07-16
申请人： Kunsheng Wang
发明人： Kunsheng Wang
IPC分类号： H02G13/00 , H01T19/00
CPC分类号： H02G13/80 , H01T4/02 , H01T19/00
摘要： A passive compound strong-ionization discharging plasma lightning rejection device. The device comprises a thundercloud charge gathering and eliminating unit, a strong-ionization discharging unit and an grounding conductor. A discharging electrode of the strong-ionization discharging unit has two poles, pole A being combined with the thundercloud charge gathering and eliminating unit into one piece, pole B being connected to the grounding conductor, and a discharging gap between the two poles. This device is excited by a thundercloud electric field , generates scores of mC/s dissipation electric charges by means of compound strong-ionization discharging, efficiently neutralizes cloud and earth electric charges ±Q gathered by the thundercloud charge gathering and eliminating unit and the grounding conductor, and effectively restrains the voltage V=Q/C of equivalent capacitance C between the cloud and the earth from increasing, without being charged by the thundercloud to the extent of being broken down by discharging towards the earth.

5. 发明授权

US07760483B2 Method and device of compound active and passive plasma for lightning protection 失效
标题翻译：用于防雷的复合有源和无源等离子体的方法和装置
公开(公告)号：US07760483B2
公开(公告)日：2010-07-20
申请号：US11547178
申请日：2005-01-27
申请人： Kunsheng Wang , Xiaobin Li
发明人： Kunsheng Wang , Xiaobin Li
IPC分类号： H01C7/12
CPC分类号： H02G13/00 , H02G13/20 , H02G13/60 , H02G13/80
摘要： A lightning protection method and device with compound actively generated plasma and passively produced plasma provide lightning protection without the need for grounding of the protected object. The device has lightning rods with a plasma piping inside. The high density plasma generated by an active plasma generator is blown through the piping, spread out at the tips of the rods and compounded with the plasma passively produced by the corona discharge on the tips under the action of lightning cloud electric field. The compound plasma dissipates the electric charges, on the tips of the rods, inducted by the lightning cloud electric field, and neutralizes the electric charges of the lightning, preventing a thunderstruck to a protected object related to the rods.
摘要翻译：具有复合主动产生的等离子体和被动产生等离子体的雷电保护方法和装置提供防雷保护，而不需要保护对象的接地。该装置具有内部具有等离子体管道的避雷针。由活性等离子体发生器产生的高密度等离子体通过管道吹出，在棒的尖端处展开，并与雷电云电场作用下由尖端上的电晕放电被动产生的等离子体混合。复合等离子体消散由雷电云电场引起的杆尖上的电荷，并且中和闪电的电荷，防止对与棒相关的受保护物体的雷击。

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