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1. 发明申请

US20120054923A1 METHOD FOR CULTIVATION OF GENETICALLY-MODIFIED PLANT 有权
标题翻译：基因改造植物的培育方法
公开(公告)号：US20120054923A1
公开(公告)日：2012-03-01
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H5/10 , A01G31/00 , A01H5/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

2. 发明授权

US09353379B2 Method for cultivation of genetically-modified plant 有权
标题翻译：转基因植物栽培方法
公开(公告)号：US09353379B2
公开(公告)日：2016-05-31
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H1/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N15/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

3. 发明申请

US20110111144A1 INK JET RECORDING MEDIUM AND PROCESS FOR PRODUCING THE INK JET RECORDING MEDIUM 审中-公开
标题翻译：喷墨记录介质和制造喷墨记录介质的过程
公开(公告)号：US20110111144A1
公开(公告)日：2011-05-12
申请号：US12934486
申请日：2009-03-25
申请人： Masafumi Wasai , Takayuki Sato , Masaya Tosaka , Kei Takigawa
发明人： Masafumi Wasai , Takayuki Sato , Masaya Tosaka , Kei Takigawa
IPC分类号： B41M5/50 , B05D1/36
CPC分类号： B41M5/508 , B41M5/52 , B41M5/5218 , B41M5/5227 , B41M5/5245 , B41M5/5254
摘要： An inkjet recording medium comprising an ink receiving layer formed by applying a coating layer containing a pigment and a binder to at least one side of a support having air permeability, and applying a coagulation solution for coagulating the binder on a surface of the coating layer by a coagulation cast coating method, wherein a boron compound, a cationic resin and cationic colloidal silica contained in the coagulation solution exist on the surface of the ink receiving layer.
摘要翻译：一种喷墨记录介质，包括通过将含有颜料和粘合剂的涂层施加到具有透气性的载体的至少一侧而形成的油墨接收层，并且通过以下方式施加凝结溶液以将粘合剂凝结在涂层的表面上：凝结浇铸涂布方法，其中在所述油墨接收层的表面上存在包含在所述凝固溶液中的硼化合物，阳离子树脂和阳离子胶体二氧化硅。

4. 发明申请

US20100291323A1 INKJET RECORDING MEDIUM AND METHOD FOR MANUFACTURING THE SAME 审中-公开
标题翻译：喷墨记录介质及其制造方法
公开(公告)号：US20100291323A1
公开(公告)日：2010-11-18
申请号：US12680677
申请日：2008-09-29
申请人： Masafumi Wasai , Masaya Tosaka , Hiroshi Koyamoto , Kei Takigawa , Takayuki Sato , Atsushi Ono , Yoshio Yoshida
发明人： Masafumi Wasai , Masaya Tosaka , Hiroshi Koyamoto , Kei Takigawa , Takayuki Sato , Atsushi Ono , Yoshio Yoshida
IPC分类号： B32B5/16 , B05D1/36
CPC分类号： B41M5/5218 , D21H19/38
摘要： An inkjet recording medium comprising an ink-receiving layer containing a pigment and a binder formed on at least one side of a base paper containing calcium carbonate as a filler and pulp by a cast coating method, wherein the ash content of the calcium carbonate in the base paper is 15-30% by mass, and the ink-receiving layer contains colloidal silica as the pigment having a primary particle diameter of 30-70 nm and a ratio of the secondary particle diameter to the primary particle diameter of 1.5-3.0.
摘要翻译：一种喷墨记录介质，其包含含有颜料的油墨接收层和在含有碳酸钙作为填料的纸的至少一面上形成的粘合剂，通过流延涂布法形成纸浆，其中，碳酸钙的灰分含量原纸为15〜30质量％，油墨接受层含有作为颜料的胶体二氧化硅，初级粒径为30〜70nm，二次粒径与一次粒径的比例为1.5〜3.0。

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