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1. 发明申请

US20170211120A1 Pathogen Identification in Complex Biological Fluids 审中-公开
公开(公告)号：US20170211120A1
公开(公告)日：2017-07-27
申请号：US15410948
申请日：2017-01-20
申请人： Robert K. Ernst , David Goodlett , Lisa Leung
发明人： Robert K. Ernst , David Goodlett , Lisa Leung
IPC分类号： C12Q1/04 , H01J49/00 , G01N33/92 , G01N27/62 , C12Q1/18 , G01N33/49
CPC分类号： C12Q1/04 , C12Q1/18 , G01N33/49 , G01N33/6848 , G01N33/92 , G01N2333/195
摘要： Provided herein are methods for rapidly identifying a microbe, such as a pathogen, from a biological sample including, blood, urine, wound effluent, stool, serum, and bronchoalveolar lavage fluid. The method comprises obtaining the sample from the subject and performing a spectrometric analysis of the lipids in the microbe to obtain a profile. The profile obtained is compared with a molecular mass lipid profile of known microbes for identification. Also provided is a method for identifying one or more antimicrobial drugs effective to treat a microbial strain in a subject.

2. 发明申请

US20060008851A1 Methods for rapid and quantitative proteome analysis 审中-公开
公开(公告)号：US20060008851A1
公开(公告)日：2006-01-12
申请号：US11223269
申请日：2005-09-09
申请人： Rudolf Aebersold , David Goodlett
发明人： Rudolf Aebersold , David Goodlett
IPC分类号： G01N33/53
CPC分类号： G01N33/6803
摘要： The invention provides methods for identifying a polypeptide. The method can include determining two or more characteristics associated with the polypeptide, or a fragment thereof, one of the characteristics being mass of a fragment of the polypeptide, the fragment mass being determined by mass spectrometry; comparing the characteristics associated with the polypeptide to an annotated polypeptide index; and identifying one or more polypeptides in the annotated polypeptide index having the characteristics. The method can further include the steps of determining one or more additional characteristics associated with the polypeptide; comparing the determined characteristics to the annotated polypeptide index; optionally repeating the steps one or more times, wherein a set of characteristics is determined that identifies a single polypeptide in the annotated polypeptide index, and optionally quantitating the amount of identified polypeptide in a sample containing the polypeptide. The invention also provides methods for generating a polypeptide identification index.

3. 发明申请

US20060020393A1 Methods for quantification and de novo polypeptide sequencing by mass spectrometry 审中-公开
公开(公告)号：US20060020393A1
公开(公告)日：2006-01-26
申请号：US11004570
申请日：2004-12-02
申请人： David Goodlett , Andrew Keller
发明人： David Goodlett , Andrew Keller
IPC分类号： G01N33/53
CPC分类号： G01N33/6848
摘要： The invention provides a method of determining an amino acid sequence of a parent polypeptide. The method consists of: (a) obtaining mass spectra of two or more differentially labeled polypeptide fragments of a parent polypeptide; (b) assigning a mass and a weighting characteristic to two or more paired signals having a difference in mass corresponding to an integer value of said differential label, the weighting characteristic combining properties of each signal within said paired signals; (c) selecting from the mass spectra a paired signal having the assigned mass and a weighting characteristic distinguishable from non-peptide signals, the assigned mass indicating the mass of a polypeptide fragment within the spectra; (d) determining the difference in mass of the polypeptide fragments; (e) assigning the mass differences a satisfying amino acid name, and (f) orienting the assigned amino acid names. Also provided is a method of determining the amino acid sequence of a polypeptide. The method consists of: (a) constructing a graph from mass spectra of two or more differentially labeled polypeptides, the graph comprising a node with mass m, number of labels n, intensity i, and mass differential of labels δ; (b) creating a node corresponding to a paired signal having masses of about m and about m+nδ, and (c) adding a labeled weighted directed edge to the graph between any two nodes corresponding to a mass of an amino acid, the labeled weighted directed edge combining properties of the paired signals.

4. 发明申请

US20060009915A1 Rapid and quantitative proteome analysis and related methods 有权
公开(公告)号：US20060009915A1
公开(公告)日：2006-01-12
申请号：US11225265
申请日：2005-09-12
申请人： David Goodlett
发明人： David Goodlett
IPC分类号： G06F19/00 , G01N33/00
CPC分类号： G01N33/6848 , G01N33/6803 , G01N2570/00
摘要： The invention provides methods for identifying polypeptides. The method can include the steps of simultaneously determining the mass of a subset of parent polypeptides from a population of polypeptides and the mass of fragments of the subset of parent polypeptides; comparing the determined masses to an annotated polypeptide index; and identifying one or more polypeptides of the annotated polypeptide index having the determined masses. The method can further include the steps of determining one or more additional characteristics associated with one or more of the parent polypeptides; comparing the determined characteristics to the annotated polypeptide index; and optionally repeating the steps one or more times, wherein a set of characteristics is determined that identifies a parent polypeptide as a single polypeptide in the annotated polypeptide index. The method can additionally include the step of quantitating the amount of the identified polypeptide in a sample containing the polypeptide.

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