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    • 1. 发明授权
    • Child seat mounting system
    • 儿童座椅安装系统
    • US6095604A
    • 2000-08-01
    • US407524
    • 1999-09-28
    • Robert J. StackJohn M. Bederka
    • Robert J. StackJohn M. Bederka
    • B60N2/28A47C1/08
    • B60N2/286B60N2/2809B60N2/2839
    • A vehicle seat is provided which has a cushion portion for alternately supporting a seated occupant or a bottom portion of a child seat. The vehicle seat also has a seatback portion with a rearward facing surface and a forward facing surface for alternately supporting a back region of a vehicle seat occupant or a seatback of a child seat. A connector receptacle is recess mounted into the rearwardly facing surface of the vehicle seatback to permit engagement with a connector extending from the seatback portion of a child's seatback. The connector receptacle has a bar onto which a hook of the connector attaches.
    • 提供一种车座,其具有用于交替地支撑座椅的乘客或儿童座椅的底部的缓冲部分。 车辆座椅还具有座椅靠背部分,其具有向后表面和向前表面,用于交替地支撑车辆座椅乘员的后部区域或儿童座椅的座椅靠背。 连接器插座被凹入安装到车辆座椅靠背的向后的表面中,以允许与从儿童座椅靠背的座椅靠背部分延伸的连接器接合。 连接器插座具有杆,连接器的钩附接到该杆上。
    • 2. 发明授权
    • Two-dimensional electrophoretic separation of carbohydrates
    • 碳水化合物的二维电泳分离
    • US5094740A
    • 1992-03-10
    • US591360
    • 1990-09-27
    • Brian K. BrandleyRobert J. Stack
    • Brian K. BrandleyRobert J. Stack
    • C07H1/06C13B20/18G01N27/447
    • C13B20/18C07H1/06G01N27/44726G01N27/44773
    • Separation methodology is disclosed which allows for the separation of mixtures of carbohydrates into highly resolved detectable bands of carbohydrates. The method involves first reacting a mixture of carbohydrates with charge generating moieties which are capable of fluorescing such as 1-amino-4-naphthalene sulfonic acid (ANSA) to form carbohydrate conjugates. The conjugates are subjected to a first-dimensional gel electrophoresis in a first direction to provide separate bands of carbohydrates in the gel. A band in the gel is removed and subjected to second-dimensional electrophoresis in a second direction which is substantially perpendicular to the first direction. More specific bands of more highly resolved carbohydrates are then formed in the second-dimensional gel. The more specific bands within the second-dimensional gel are then electro-blotted onto a substrate surface and can be viewed in extremely small amounts due to the fluorescent capability of the ANSA when viewed under ultraviolet light.
    • 公开了分离方法,其允许将碳水化合物的混合物分离成高度分辨的可检测碳水化合物带。 该方法首先使碳水化合物的混合物与能够发荧光的电荷产生部分如1-氨基-4-萘磺酸(ANSA)反应形成碳水化合物缀合物。 将缀合物在第一方向进行第一维凝胶电泳,以在凝胶中提供单独的碳水化合物带。 去除凝胶中的带,并在基本上垂直于第一方向的第二方向进行第二维电泳。 然后在第二维凝胶中形成更高分辨率的碳水化合物的更具体的带。 然后将第二维凝胶中更特异的条带电印到基材表面上,并且由于在紫外光下观察时由于ANSA的荧光能力,可以以极小的量观察。
    • 3. 发明授权
    • Fluorescent tag for sugar electrophoresis
    • 荧光标记用于糖电泳
    • US5087337A
    • 1992-02-11
    • US589441
    • 1990-09-27
    • Brian K. BrandleyMichael TiemeyerRobert J. Stack
    • Brian K. BrandleyMichael TiemeyerRobert J. Stack
    • G01N27/447
    • G01N27/44726
    • A method of separating mixtures of saccharides into distinct detectable groups is disclosed. In accordance with the method a tri-functional conjugate must first be provided. The tri-functional conjugate is obtained by reacting a mixture of saccharides with moieties (a) capable of providing a charge upon ionization; (b) capable of fluorescing under ultraviolet light; and (c) having a light-activatable azido group thereon. The different functional moieties may all be present on the same moiety and connected directly to the saccharide or may be connected to each other wherein only one of the moieties is connected directly to the saccharide. The tri-functional conjugates are subjected to electrophoretic separation to obtain separate groups of conjugates in the gel. The groups of conjugates are transferred from the gel to the surface of a membrane which is exposed to light for a sufficient time and light frequency to activate the azido-group. The light-activated azido-groups attached to the surface of the membrane. The tri-functional conjugates can then be contacted with labeled probes such as radiolabeled proteins to determine the affinity of the probes to particular saccharides.
    • 公开了将糖的混合物分离成不同的可检测基团的方法。 必须首先提供三功能缀合物。 通过使糖类的混合物与能够在电离时提供电荷的部分(a)反应获得三官能偶联物; (b)能够在紫外光下发荧光; 和(c)其上具有可光活化的叠氮基。 不同的官能部分可以全部存在于相同的部分上并且直接连接到糖上,或者可以彼此连接,其中只有一个部分直接连接到糖上。 将三官能共轭物进行电泳分离以在凝胶中获得单独的缀合物组。 缀合物组从凝胶转移到膜的表面,其暴露于光下足够的时间和光频率以活化叠氮基。 附着在膜表面的光激活的叠氮基。 然后可以将三功能缀合物与标记的探针例如放射性标记的蛋白质接触,以确定探针对特定糖的亲和力。