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1. 发明申请

US20180051318A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 审中-公开
公开(公告)号：US20180051318A1
公开(公告)日：2018-02-22
申请号：US15714210
申请日：2017-09-25
申请人： Promega Corporation
发明人： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC分类号： C12Q1/66 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

2. 发明授权

US09777311B2 Synthetic Oplophorus luciferases with enhanced light output 有权
公开(公告)号：US09777311B2
公开(公告)日：2017-10-03
申请号：US14053252
申请日：2013-10-14
申请人： PROMEGA CORPORATION
发明人： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC分类号： C12Q1/66 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

3. 发明授权

US09757478B2 Mutant protease biosensors with enhanced detection characteristics 有权
公开(公告)号：US09757478B2
公开(公告)日：2017-09-12
申请号：US14990279
申请日：2016-01-07
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： A61K49/00 , C12N9/02 , C12Q1/37 , C12Q1/66 , A01K67/027
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

4. 发明申请

US20140120548A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
标题翻译：具有增强光输出功能的合成眼科植入物
公开(公告)号：US20140120548A1
公开(公告)日：2014-05-01
申请号：US14053252
申请日：2013-10-14
申请人： PROMEGA CORPORATION
发明人： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC分类号： C12Q1/66
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译：编码经修饰的荧光素酶多肽的多核苷酸。修饰的荧光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus荧光素酶中的氨基酸的位置包括至少一个氨基酸取代。修饰的荧光素酶多肽相对于野生型Oplophorus萤光素酶具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。

5. 发明授权

US11365436B2 Synthetic oplophorus luciferases with enhanced light output 有权
公开(公告)号：US11365436B2
公开(公告)日：2022-06-21
申请号：US17075415
申请日：2020-10-20
申请人： Promega Corporation
发明人： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC分类号： C12Q1/66 , C12N9/00 , C12N9/02
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

6. 发明授权

US09593316B2 Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression 有权
标题翻译：编码突变体水解酶蛋白的多核苷酸具有增强的动力学和功能表达
公开(公告)号：US09593316B2
公开(公告)日：2017-03-14
申请号：US14285327
申请日：2014-05-22
申请人： Promega Corporation
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
IPC分类号： C07H21/00 , C12N1/21 , C12N1/15 , C12N9/14 , C12Q1/34 , G01N33/58
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
摘要翻译：本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。

7. 再颁专利

USRE46326E1 RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags 有权
标题翻译： RF销售和交付方式以及与远程计算机通信的系统，具有阅读大量RF标签的功能
公开(公告)号：USRE46326E1
公开(公告)日：2017-02-28
申请号：US14602694
申请日：2015-01-22
申请人： Promega Corporation
发明人： Michael Dearing , Gediminas Vidugiris , William A. Linton , John Linton , Julia E. Krueger
IPC分类号： G06Q30/00 , G06K7/10 , G07G1/00
CPC分类号： G06Q10/08 , F25D2500/06 , F25D2700/08 , G06F19/00 , G06K7/10336 , G06K2017/0051 , G06K2017/0067 , G06Q10/087 , G06Q30/06 , G06Q30/0603 , G07F9/026 , G07G1/0045
摘要： A method and system for providing point-of-sale and point-of-delivery and/or distribution of products in a restricted access unit near the customer. The method and system utilize products equipped with radio frequency tags and reduce the effects of energy sharing, shadowing, and nulls. In one embodiment, a plurality of RF tagged products are placed within a refrigerator, cabinet, or other micro-warehouse that has a door or opening that can detect access to the micro-warehouse. In one embodiment, one or more antennas are positioned within the door. Each antenna may have a transmission line of sight and be configured to emit a signal at predefined frequencies. Each antenna generates an electromagnetic field within the micro-warehouse. In one embodiment, the products are positioned in one or more bins, compartments, or similar devices located within the micro-warehouse such that at least two of the plurality of products are spaced a distance from each other to reduce energy sharing. The electromagnetic field is moved or altered within the micro-warehouse through the use of reflectors, devices that move the antennas, or other mechanisms.
摘要翻译：一种用于在客户附近的受限访问单元中提供销售点和交货点和/或分发产品的方法和系统。该方法和系统利用配备射频标签的产品，减少能量共享，阴影和零点的影响。在一个实施例中，将多个RF标签的产品放置在具有门或开口的冰箱，橱柜或其他微型仓库内，该门或开口可以检测对微型仓库的通道。在一个实施例中，一个或多个天线位于门内。每个天线可以具有传输线，并被配置为以预定的频率发射信号。每个天线在微型仓库内产生一个电磁场。在一个实施例中，将产品定位在位于微仓库内的一个或多个仓，隔间或类似装置中，使得多个产品中的至少两个彼此间隔开一定距离以减少能量共享。通过使用反射器，移动天线的装置或其他机构，在微型仓库内移动或改变电磁场。

8. 发明授权

US09339561B2 Mutant protease biosensors with enhanced detection characteristics 有权
标题翻译：具有增强检测特性的突变蛋白酶生物传感器
公开(公告)号：US09339561B2
公开(公告)日：2016-05-17
申请号：US14269669
申请日：2014-05-05
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： A61K49/00 , C12N9/02 , C12Q1/37 , C12Q1/66 , A01K67/027
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码包含修饰的环状置换的热稳定性荧光素酶的生物传感器多肽的多核苷酸和将热稳定性荧光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。经修饰的环状置换的热稳定荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

9. 发明授权

US09290794B2 Mutant protease biosensors with enhanced detection characteristics 有权
公开(公告)号：US09290794B2
公开(公告)日：2016-03-22
申请号：US13674655
申请日：2012-11-12
申请人： PROMEGA CORPORATION
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Kimberly Risma , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： C12Q1/66 , C12Q1/37 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0069 , C12Q1/37
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

10. 发明申请

US20220389434A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
公开(公告)号：US20220389434A1
公开(公告)日：2022-12-08
申请号：US17844371
申请日：2022-06-20
申请人： Promega Corporation
发明人： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC分类号： C12N15/52
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

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