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1. 发明授权

US07897373B2 Construction of new variants of dextransucrase DSR-S by genetic engineering 有权
标题翻译：通过基因工程构建葡聚糖蔗糖酯酶DSR-S的新变体
公开(公告)号：US07897373B2
公开(公告)日：2011-03-01
申请号：US12223744
申请日：2007-02-08
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C12N9/24 , C07H21/04
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及一种在保护酶活性或其合成α-1,6键的特异性的同时生产截短或突变的葡聚糖蔗糖酶的重组方法。本发明涉及截短或突变的葡聚糖蔗糖酶的核酸序列，含有核酸序列的载体和由编码截短或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。另一方面，本发明涉及一种以重组方式生产截短或突变的葡聚糖蔗糖酯的方法，其保留其酶活性或在合成α-1,6键时保护其特异性，并可从蔗糖，葡萄糖，与用天然酶和具有受控摩尔质量的异麦芽低聚糖和葡聚糖获得的葡聚糖的性质相比，具有高的摩尔质量和改进的流变学性质。本发明的葡聚糖和异麦芽低聚糖可以用作变形剂或益生元。

2. 发明申请

US20120289689A1 CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING 审中-公开
标题翻译：通过遗传工程构建新型番茄红素DSR-S的新品种
公开(公告)号：US20120289689A1
公开(公告)日：2012-11-15
申请号：US13564776
申请日：2012-08-02
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C12N15/54 , C12N15/63 , C12N1/21 , C07H3/06 , C12N1/19 , C12N5/10 , C12P19/18 , C08B37/02 , C12N9/10 , C12N1/11
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及一种在保护酶活性或其合成α-1,6键的特异性的同时生产截短或突变的葡聚糖蔗糖酶的重组方法。本发明涉及截短或突变的葡聚糖蔗糖酶的核酸序列，含有核酸序列的载体和由编码截短或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。另一方面，本发明涉及一种以重组方式生产截短或突变的葡聚糖蔗糖酯的方法，其保留其酶活性或在合成α-1,6键时保护其特异性，并可从蔗糖，葡萄糖，与用天然酶和具有受控摩尔质量的异麦芽低聚糖和葡聚糖获得的葡聚糖的性质相比，具有高的摩尔质量和改进的流变学性质。本发明的葡聚糖和异麦芽低聚糖可以用作变形剂或益生元。

3. 发明申请

US20110178289A1 Construction of new variants of dextransucrase DSR-S by genetic engineering 有权
公开(公告)号：US20110178289A1
公开(公告)日：2011-07-21
申请号：US12930748
申请日：2011-01-14
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C07H3/06 , C07H21/04 , C12N15/63 , C12N1/21 , C12N9/46 , C12P19/04 , C12P19/08
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.

4. 发明申请

US20090258389A1 Construction of New Variants of Dextransucrase DSR-S by Genetic Engineering 有权
标题翻译：通过遗传工程构建葡聚糖酶DSR-S的新变异
公开(公告)号：US20090258389A1
公开(公告)日：2009-10-15
申请号：US12223744
申请日：2007-02-08
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C12P21/00 , C07H21/02 , C12N15/74 , C12N1/00 , C07K14/00 , C12N9/26 , C08B37/02 , C07H1/00
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the α-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of α-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及用于在保护其酶活性和/或其在α-1,6键合成中的特异性的同时产生截短和/或突变的葡聚糖蔗糖酶的重组方法。更准确地说，本发明涉及截短和/或突变的葡聚糖蔗糖酶的核酸序列，含有所述核酸序列的载体和通过编码截短和/或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。在另一方面，本发明涉及以重组方式生产保留其酶活性和/或在α-1,6键的合成中保护其特异性的截短和/或突变的葡聚糖蔗糖酶的方法，然而可产生与来自蔗糖的葡聚糖，具有高摩尔质量和具有改进的流变性质的葡聚糖，与在相同条件下用天然酶获得的葡聚糖的性质和具有受控摩尔质量的异麦芽低聚糖和葡聚糖相比。本发明的葡聚糖和海藻酸盐可以用作变形剂或益生元。

5. 发明授权

US09109207B2 Construction of new variants of dextransucrase DSR-S by genetic engineering 有权
标题翻译：通过基因工程构建葡聚糖蔗糖酯酶DSR-S的新变体
公开(公告)号：US09109207B2
公开(公告)日：2015-08-18
申请号：US13564776
申请日：2012-08-02
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C12N9/10 , C12N9/24 , C12N9/46 , C12P21/06 , C12P19/34 , C12N1/20 , C12N1/00 , C12P19/18 , C12P19/08 , C12N15/00 , C07H21/04 , C08B37/02 , C12P19/04
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及一种在保护酶活性或其合成α-1,6键的特异性的同时生产截短或突变的葡聚糖蔗糖酶的重组方法。本发明涉及截短或突变的葡聚糖蔗糖酶的核酸序列，含有核酸序列的载体和由编码截短或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。另一方面，本发明涉及一种以重组方式生产截短或突变的葡聚糖蔗糖酯的方法，其保留其酶活性或在合成α-1,6键时保护其特异性，并可从蔗糖，葡萄糖，与用天然酶和具有受控摩尔质量的异麦芽低聚糖和葡聚糖获得的葡聚糖的性质相比，具有高的摩尔质量和改进的流变学性质。本发明的葡聚糖和异麦芽低聚糖可以用作变形剂或益生元。

6. 发明授权

US08263380B2 Construction of new variants of dextransucrase DSR-S by genetic engineering 有权
标题翻译：通过基因工程构建葡聚糖蔗糖酯酶DSR-S的新变体
公开(公告)号：US08263380B2
公开(公告)日：2012-09-11
申请号：US12930748
申请日：2011-01-14
申请人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
发明人： Pierre F. Monsan , Magali Remaud-Simeon , Gabrielle Potocki-Veronese , Claire Moulis
IPC分类号： C12N9/24 , C12N9/46 , C12P19/34 , C12N15/00 , C12N1/20 , C12P19/04 , C12P19/08
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及一种在保护酶活性或其合成α-1,6键的特异性的同时生产截短或突变的葡聚糖蔗糖酶的重组方法。本发明涉及截短或突变的葡聚糖蔗糖酶的核酸序列，含有核酸序列的载体和由编码截短或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。另一方面，本发明涉及以重组方式生产截短或突变的葡聚糖蔗糖酯，其保留其酶活性或保护其在α-1,6键的合成中的特异性，并且可以从蔗糖，葡聚糖，与用天然酶和具有受控摩尔质量的异麦芽低聚糖和葡聚糖获得的葡聚糖的性质相比，具有高的摩尔质量和改进的流变学性质。本发明的葡聚糖和异麦芽低聚糖可以用作变形剂或益生元。

7. 发明申请

US20110144317A1 MUTANTS OF GLYCOSIDE HYDROLASES AND USES THEREOF FOR SYNTHESIZING COMPLEX OLIGOSACCHARIDES AND DISACCHARIDE INTERMEDIATES 审中-公开
标题翻译：甘油三酯水解物及其用于合成复合寡糖和不对称中间体的用途
公开(公告)号：US20110144317A1
公开(公告)日：2011-06-16
申请号：US12922113
申请日：2009-03-12
申请人： Laurence Mulard , Isabelle Andre , Elise Champion , Claire Moulis , Sandrine Morel , Pierre Monsan , Magali Remaud-Simeon , Julien Boutet
发明人： Laurence Mulard , Isabelle Andre , Elise Champion , Claire Moulis , Sandrine Morel , Pierre Monsan , Magali Remaud-Simeon , Julien Boutet
IPC分类号： C12P19/12 , C12N9/10 , C12N9/24 , C07H21/00 , C12N15/63 , C12P19/26 , C07H15/04 , C07H13/02 , C07H9/04
CPC分类号： C12P19/16 , C12N9/1051 , C12N9/2402 , C12P19/18 , C12Y204/01004 , C12Y302/01048
摘要： Method for preparing the disaccharide [α-D-Gldp(1→3)]-α-L-Rhap-YR wherein Y is selected from —O— and —S— and R is selected from the group consisting of: C1-C6 alkyl, C1-C6 alkenyl, aryl, allyl, —CO-alkyl (C1-C6), —CO-alkenyl (C1-C6), —CO-aryl comprising the step of using a mutant of a wild type glycoside hydrolase.
摘要翻译：制备二糖[α-D-Gldp（1→3）] -a-L-Rhap-YR的方法，其中Y选自-O-和-S-，R选自：C 1 -C 6 烷基，C 1 -C 6烯基，芳基，烯丙基，-CO-烷基（C 1 -C 6），-CO-烯基（C 1 -C 6），-CO-芳基，包括使用野生型糖苷水解酶突变体的步骤。

8. 发明申请

US20110086767A1 MUTANTS OF GLYCOSIDE HYDROLASES AND USES THEREOF FOR SYNTHESIZING COMPLEX OLIGOSACCHARIDES AND DISACCHARIDE INTERMEDIATES 审中-公开
标题翻译：甘油三酯水解物及其用于合成复合寡糖和不对称中间体的用途
公开(公告)号：US20110086767A1
公开(公告)日：2011-04-14
申请号：US12922108
申请日：2009-03-12
申请人： Laurence Mulard , Isabelle Andre , Elise Champion , Claire Moulis , Sandrine Morel , Pierre Monsan , Magali Remaud-Simeon , Karine Descroix
发明人： Laurence Mulard , Isabelle Andre , Elise Champion , Claire Moulis , Sandrine Morel , Pierre Monsan , Magali Remaud-Simeon , Karine Descroix
IPC分类号： C40B30/02 , C12P19/12 , C12N9/24 , C07H21/04 , C12N15/63 , C07H15/04
CPC分类号： C07H15/10 , C12N9/1051 , C12P19/16 , C12P19/18 , C12P19/26 , C12Y204/01004
摘要： Method for preparing the disaccharide α-D-glucopyranosyl-(1→4)-2-N-acetyl-2-deoxy-α-D-glucopyranoside, comprising the step of using a mutant of a wild type glycoside hydrolase.
摘要翻译：制备二糖α-D-吡喃葡萄糖基 - （1→4）-2-N-乙酰基-2-脱氧-α-D-吡喃葡萄糖苷的方法，包括使用野生型糖苷水解酶突变体的步骤。

9. 发明授权

US07618951B2 Use of prebiotics, preferably glucooligosaccharide, for the prevention of the onset of type II diabetes 有权
标题翻译：使用益生元，优选葡糖低聚糖，用于预防II型糖尿病的发病
公开(公告)号：US07618951B2
公开(公告)日：2009-11-17
申请号：US10527819
申请日：2003-09-12
申请人： Pierre Monsan , Philippe Valet , Magali Remaud-Simeon , Jean-Sebastien Saulnier-Blache , Remy Burcelin
发明人： Pierre Monsan , Philippe Valet , Magali Remaud-Simeon , Jean-Sebastien Saulnier-Blache , Remy Burcelin
IPC分类号： A61K31/715 , A61K31/702 , A61K31/70 , A61K31/7016
CPC分类号： A61K31/716 , A23L29/35 , A23L33/10 , A23V2002/00 , A61K31/702 , A61K2300/00 , A23V2250/606 , A23V2250/61 , A23V2250/616 , A23V2250/628 , A23V2250/5114 , A23V2250/28
摘要： The invention relates to the use of prebiotics for the preparation of food or pharmaceutical compositions which are intended for the treatment and/or prevention of hyperglycaemic syndromes and, in particular, for the treatment of Type II diabetes and/or the prevention of the onset of Type II diabetes in patients with a predisposition to developing the type of diabetes. The invention also relates to the food and pharmaceutical compositions containing the prebiotics.
摘要翻译：本发明涉及益生元用于制备用于治疗和/或预防高血糖综合征的食物或药物组合物的用途，特别是用于治疗II型糖尿病和/或预防患有糖尿病发病倾向的II型糖尿病患者。本发明还涉及含有益生元的食品和药物组合物。

10. 发明申请

US20060127328A1 Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof 有权
公开(公告)号：US20060127328A1
公开(公告)日：2006-06-15
申请号：US11011702
申请日：2004-12-14
申请人： Pierre Monsan , Magali Remaud-Simeon , Gilles Joucla
发明人： Pierre Monsan , Magali Remaud-Simeon , Gilles Joucla
IPC分类号： A61K8/96 , C07H21/04 , C12P21/06 , C12N9/24
CPC分类号： C12P19/04 , C12N9/1051 , C12Y204/0114
摘要： Nucleic acid sequences of truncated or mutated alternansucrases, vectors containing these nucleic acids sequences, host cells transformed with the nucleic acid sequences encoding truncated or mutated alternansucrases are provided. Furthermore, a process to recombinantly alternansucrase with a high level of expression, while retaining the enzymatic activity is described.

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