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    • 1. 发明申请
    • Transgenic Plants with Enhanced Growth Characteristics
    • 具有增强生长特性的转基因植物
    • US20100186121A1
    • 2010-07-22
    • US12551271
    • 2009-08-31
    • Pat J. UnkeferPenelope S. AndersonThomas J. Knight
    • Pat J. UnkeferPenelope S. AndersonThomas J. Knight
    • A01H5/00C12N15/82
    • C12N9/1096C12N15/8261Y02A40/146
    • The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.
    • 本发明涉及显着增强的生长速率,更大的种子和果实/荚果产量,更早和更有效的开花,更有效的氮利用,增加的对高盐条件的耐受性和增加的生物量产量的转基因植物。 在一个实施方案中,提供了改造为过表达谷氨酰胺苯丙酮酸转氨酶(GPT)和谷氨酰胺合成酶(GS)的转基因植物。 本发明的GPT + GS双转基因植物始终表现出增强的生长特性,其中T0代系显示生物量比野生型对应物的增加在50%至300%之间。 由性交和/或自交产生的一代通常表现得更好,一些双转基因植物比野生型植物实现惊人的四倍生物量增加。
    • 9. 发明授权
    • Use of prolines for improving growth and other properties of plants and algae
    • 使用脯氨酸来改善植物和藻类的生长和其他性质
    • US06593275B1
    • 2003-07-15
    • US10124568
    • 2002-04-16
    • Pat J. UnkeferThomas J. KnightRodolfo A. Martinez
    • Pat J. UnkeferThomas J. KnightRodolfo A. Martinez
    • A01N4336
    • A01N43/36Y02P60/246
    • Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.
    • 植物叶片中增加脯氨酸浓度,如2-羟基-5-氧代脯氨酸,已被证明导致二氧化碳固定,生长速率,干重,营养价值(氨基酸),结瘤和氮素的增加 固定,光合作用衍生的化学能,以及对野生型植物具有相同性质的抗病虫害。 这可以通过四种方式实现:(1)通过喷洒这些部分将脯氨酸溶液直接施加到植物的叶面部分; (2)将脯氨酸溶液施用于植物根部; (3)对植物进行遗传工程和筛选,以产生过量表达叶中谷氨酰胺合成酶的产生线,从而导致代谢物浓度增加,2-羟基-5-氧代脯氨酸(该脯氨酸也称为2-氧代谷氨酸)。 (4)损害植物根系中的谷氨酰胺合成酶活性,导致叶中谷氨酰胺合成酶活性升高,导致2-羟基-5-氧代脯氨酸浓度增加。 脯氨酸也被发现在藻类中诱发类似的作用。
    • 10. 发明授权
    • Use of prolines for improving growth and other properties of plants and algae
    • 使用脯氨酸来改善植物和藻类的生长和其他性质
    • US06555500B1
    • 2003-04-29
    • US10124520
    • 2002-04-16
    • Pat J. UnkeferThomas J. KnightRodolfo A. Martinez
    • Pat J. UnkeferThomas J. KnightRodolfo A. Martinez
    • A01N4336
    • A01N43/36Y02P60/246
    • Increasing the concentration of prolines such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that overexpress glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramnate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.
    • 已经显示在植物叶面部分增加脯氨酸如2-羟基-5-氧代脯氨酸的浓度导致二氧化碳固定,生长速率,干重,营养价值(氨基酸),结瘤和固氮的增加 ,光合作用衍生的化学能,以及对野生型植物具有相同性质的抗病虫害。 这可以通过四种方式实现:(1)通过喷洒这些部分将脯氨酸溶液直接施加到植物的叶面部分; (2)将脯氨酸溶液施用于植物根部; (3)对植物进行遗传工程和筛选,以产生过度表达叶中谷氨酰胺合成酶的产生线,从而导致代谢物浓度升高,2-羟基-5-氧代脯氨酸(该脯氨酸也称为2-氧代戊二酸)。 (4)损害植物根系中的谷氨酰胺合成酶活性,导致叶中谷氨酰胺合成酶活性升高,导致2-羟基-5-氧代脯氨酸浓度增加。 脯氨酸也被发现在藻类中诱发类似的作用。