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1. 发明公开

US20230392157A1 APTAMER METHOD 审中-公开
公开(公告)号：US20230392157A1
公开(公告)日：2023-12-07
申请号：US18316852
申请日：2023-05-12
申请人： Oxford Nanopore Technologies PLC
发明人： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC分类号： C12N15/115 , G01N33/53 , G01N33/543
CPC分类号： C12N15/115 , G01N33/5308 , G01N33/54386 , G01N2333/705 , G01N2333/36 , G01N2333/49 , G01N2333/96463
摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

2. 发明公开

US20240060969A1 METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A TARGET ANALYTE COMPRISING USING A REPORTER POLYNUCLEOTIDE AND A TRANSMEMBRANE PORE 审中-公开
公开(公告)号：US20240060969A1
公开(公告)日：2024-02-22
申请号：US18454975
申请日：2023-08-24
申请人： Oxford Nanopore Technologies PLC
发明人： Daniel George Fordham
IPC分类号： G01N33/543
CPC分类号： G01N33/54306
摘要： The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.

3. 发明授权

US11685922B2 Aptamer method 有权
公开(公告)号：US11685922B2
公开(公告)日：2023-06-27
申请号：US16916305
申请日：2020-06-30
申请人： Oxford Nanopore Technologies PLC
发明人： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC分类号： G01N33/00 , G01N33/543 , G01N33/53 , C12N15/115 , C07H21/04
CPC分类号： G01N33/54386 , C12N15/115 , G01N33/5308 , G01N2333/36 , G01N2333/49 , G01N2333/705 , G01N2333/96463
摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

4. 发明公开

US20240200128A1 METHOD 审中-公开
公开(公告)号：US20240200128A1
公开(公告)日：2024-06-20
申请号：US18352701
申请日：2023-07-14
申请人： Oxford Nanopore Technologies PLC
发明人： Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC分类号： C12Q1/6869 , C12Q1/6876 , G01N33/487
CPC分类号： C12Q1/6869 , C12Q1/6876 , G01N33/48721
摘要： The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore: (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

5. 发明公开

US20230295694A1 METHOD 审中-公开
公开(公告)号：US20230295694A1
公开(公告)日：2023-09-21
申请号：US18160816
申请日：2023-01-27
申请人： Oxford Nanopore Technologies PLC
发明人： Nicholas Antony Smith , Daniel John Turner , Daniel George Fordham , James White
IPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/68 , C12Q1/6876
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/68 , C12Q1/6876 , C12Q2525/101 , C12Q2525/161 , C12Q2537/143 , C12Q2563/116 , C12Q2565/607 , C12Q2565/631
摘要： A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising:

(i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein:

(a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and
(b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides;

(ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore;
(iii) measuring current blockades having a duration within a defined window, wherein:

(a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and
(b) each hybridised probe gives rise to a current blockade indicative of that probe; and

(iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.

6. 发明授权

US11789014B2 Method of determining the presence or absence of a target analyte comprising using a reporter polynucleotide and a transmembrane pore 有权
公开(公告)号：US11789014B2
公开(公告)日：2023-10-17
申请号：US16610891
申请日：2018-05-03
申请人： Oxford Nanopore Technologies PLC
发明人： Daniel George Fordham
IPC分类号： G01N33/543
CPC分类号： G01N33/54306
摘要： The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.

7. 发明公开

US20240076719A9 METHOD 审中-公开
公开(公告)号：US20240076719A9
公开(公告)日：2024-03-07
申请号：US18160816
申请日：2023-01-27
申请人： Oxford Nanopore Technologies PLC
发明人： Nicholas Antony Smith , Daniel John Turner , Daniel George Fordham , James White
IPC分类号： C12Q1/6825 , C12Q1/68 , C12Q1/6816 , C12Q1/6876
CPC分类号： C12Q1/6825 , C12Q1/68 , C12Q1/6816 , C12Q1/6876 , C12Q2525/101 , C12Q2525/161 , C12Q2537/143 , C12Q2563/116 , C12Q2565/607 , C12Q2565/631
摘要： A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising:

(i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein:

(a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and
(b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides;

(ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore;
(iii) measuring current blockades having a duration within a defined window, wherein:

(a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and
(b) each hybridised probe gives rise to a current blockade indicative of that probe; and

(iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.

8. 发明授权

US11739379B2 Method for nucleic acid detection by guiding through a nanopore 有权
公开(公告)号：US11739379B2
公开(公告)日：2023-08-29
申请号：US17361821
申请日：2021-06-29
申请人： Oxford Nanopore Technologies PLC
发明人： Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6876 , G01N33/487
CPC分类号： C12Q1/6869 , C12Q1/6876 , G01N33/48721
摘要： The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

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