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1. 发明授权

US11280792B2 Apparatus for detecting the degree of malignancy of each of circulating tumor cells 有权
公开(公告)号：US11280792B2
公开(公告)日：2022-03-22
申请号：US16123444
申请日：2018-09-06
申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD. , SHIZUOKA PREFECTURE
发明人： Kazuo Takeda , Namiko Yamashita , Yuu Fujimura , Kaori Nishio , Yasuhiro Koh , Masaru Watanabe , Fumiaki Koizumi , Yuri Uehara
IPC分类号： G01N33/574 , C12N5/09 , G01N33/58
摘要： The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.
The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps: (a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells, (b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M).

2. 发明授权

US11826752B2 Method for purifying particles, method for dispensing a single particle, method for analyzing cell cluster, and apparatus using the same 有权
公开(公告)号：US11826752B2
公开(公告)日：2023-11-28
申请号：US17445789
申请日：2021-08-24
申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD.
发明人： Kazuo Takeda , Masayuki Ishige , Yuu Fujimura , Takahide Ino , Yuji Morishita , Kosuke Osawa , Soichiro Tsuda
IPC分类号： B01L3/00 , G01N1/30 , G01N15/14
CPC分类号： B01L3/502784 , B01L3/502715 , G01N1/30 , G01N15/1404 , G01N15/1484 , B01L2200/0673 , B01L2400/0487 , G01N2015/149
摘要： One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

3. 发明授权

US11725179B2 Single-particle analysis method, and system for performing said analysis 有权
公开(公告)号：US11725179B2
公开(公告)日：2023-08-15
申请号：US15573459
申请日：2016-05-12
申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD.
发明人： Kazuo Takeda , Yuu Fujimura , Takahide Ino , Masayuki Ishige , Jin Akagi
IPC分类号： C12Q1/68 , C12M1/00 , C12N5/07 , C12Q1/02 , G01N15/14 , B01L3/00 , C12M1/32 , C12M1/26 , C12M1/34 , C12Q1/6881 , G01N15/10
CPC分类号： C12M47/04 , B01L3/502761 , B01L3/502784 , C12M1/00 , C12M23/12 , C12M33/04 , C12M41/46 , C12M47/06 , C12N5/06 , C12Q1/02 , C12Q1/6881 , G01N15/1404 , G01N15/1434 , G01N15/1484 , B01L2200/0652 , B01L2200/0673 , B01L2300/0861 , B01L2400/0481 , B01L2400/0487 , G01N15/1459 , G01N2015/1006 , G01N2015/149
摘要： An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.
The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit,
wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

4. 发明申请

US20150064722A1 METHOD FOR DETECTING THE DEGREE OF MALIGNANCY OF EACH OF THE CIRCULATING TUMOR CELLS, AND A KIT FOR THE SAME 审中-公开
标题翻译：检测循环肿瘤细胞恶性程度的方法及其相关试剂盒
公开(公告)号：US20150064722A1
公开(公告)日：2015-03-05
申请号：US14388445
申请日：2013-03-28
申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD. , SHIZUOKA PREFECTURE
发明人： Kazuo Takeda , Namiko Yamashita , Yuu Fujimura , Kaori Nishio , Yasuhiro Koh , Masaru Watanabe , Fumiaki Koizumi , Yuri Uehara
IPC分类号： G01N33/574
CPC分类号： G01N33/57492 , C12N5/0693 , G01N33/574 , G01N33/58 , G01N33/582
摘要： The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps: (a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells, (b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M).
摘要翻译：本发明的目的是提供能够准确地确定癌症转移，癌症进展阶段或癌症恶性的评价方法。可以通过检测每个循环肿瘤细胞的恶性程度的方法来解决该问题，其特征在于以下步骤：（a）使上皮细胞结合成分与上皮细胞上表达的标志物分子特异性结合并且被荧光标记或发光酶标记，和间充质细胞结合组分，其特异性结合于在间充质细胞上表达并被荧光标记或发光酶标记的标记分子与可能含有循环的样品接触肿瘤细胞，（b）检测上皮细胞结合成分的荧光信号或发光信号，以及每个细胞的间充质细胞结合成分的荧光信号或发光信号，（c）基于上皮细胞结合组分（E）的信号量和循环肿瘤细胞的信号量的上皮 - 间质转化的间充质细胞结合成分（M）。

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