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1. 发明授权

US06784346B1 Value-added traits in grain and seed transformed with thioredoxin 失效
标题翻译：用硫氧还蛋白转化的谷物和种子的增值性状
公开(公告)号：US06784346B1
公开(公告)日：2004-08-31
申请号：US09538864
申请日：2000-03-29
申请人： Myeong-Je Cho , Peggy G. Lemaux , Bob B. Buchanan , Joshua Wong , Corina Marx
发明人： Myeong-Je Cho , Peggy G. Lemaux , Bob B. Buchanan , Joshua Wong , Corina Marx
IPC分类号： A01H500
CPC分类号： C12N15/8261 , A21D13/064 , A23J1/12 , A23K10/30 , A23L33/105 , A23V2002/00 , C07K14/415 , C12N9/0036 , C12N15/8242 , C12N15/8245 , C12N15/8251 , Y02A40/146 , A23V2300/21
摘要： Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.
摘要翻译：本文提供了组合物和使用方法以制备和使用具有增值性状的转基因植物。

2. 发明授权

US06642437B1 Production of proteins in plant seeds 有权
标题翻译：在植物种子中生产蛋白质
公开(公告)号：US06642437B1
公开(公告)日：2003-11-04
申请号：US09164210
申请日：1998-09-30
申请人： Peggy G. Lemaux , Myeong-Je Cho , Bob B. Buchanan
发明人： Peggy G. Lemaux , Myeong-Je Cho , Bob B. Buchanan
IPC分类号： C12N504
CPC分类号： C12N15/8257 , C12N15/8201 , C12N15/8207 , C12N15/8216 , C12N15/8221 , C12N15/8222 , C12N15/8234 , C12N15/8242
摘要： Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein.
摘要翻译：公开了在植物种子中产生蛋白质的方法。蛋白质的表达由种子特异性启动子驱动，并且蛋白质优选表达为融合多肽，其包括使蛋白质积聚在亚细胞隔室中以保护蛋白质的信号肽。

3. 发明授权

US06380372B1 Barley gene for Thioredoxin and NADP-thioredoxin reductase 失效
标题翻译：用于硫氧还蛋白和NADP-硫氧还蛋白还原酶的大麦基因
公开(公告)号：US06380372B1
公开(公告)日：2002-04-30
申请号：US09540014
申请日：2000-03-31
申请人： Myeong-Je Cho , Gregorio del Val , Maxime Caillau , Peggy G. Lemaux , Bob B. Buchanan
发明人： Myeong-Je Cho , Gregorio del Val , Maxime Caillau , Peggy G. Lemaux , Bob B. Buchanan
IPC分类号： C07H2102
CPC分类号： C12N15/8251 , A21D13/064 , A23J1/12 , A23L33/105 , A23V2002/00 , C07K14/415 , C12N9/0036 , C12N15/8242 , C12N15/8245 , C12N15/8261 , Y02A40/146 , A23V2300/21
摘要： The present invention provides barley thioredoxin h nucleic acids and proteins. The barley thioredoxin h nucleic acid may be isolated or it may be an expression vector. The expression vector may be operably linked to a transcriptional regulatory sequence. The invention also provides for transgenic plants comprising recombinant barley thioredoxin h. The invention also provides methods of expressing and purifying barley thioredoxin h.
摘要翻译：本发明提供大麦硫氧还蛋白h核酸和蛋白质。大麦硫氧还蛋白h核酸可以是分离的，也可以是表达载体。表达载体可以可操作地连接到转录调节序列。本发明还提供包含重组大麦硫氧还蛋白h的转基因植物。本发明还提供了表达和纯化大麦硫氧还蛋白h的方法。

4. 发明授权

US07179964B2 Transgenic plants with elevated thioredoxin levels 失效
标题翻译：具有升高的硫氧还蛋白水平的转基因植物
公开(公告)号：US07179964B2
公开(公告)日：2007-02-20
申请号：US10194885
申请日：2002-07-12
申请人： Myeong-Je Cho , Joshua H. Wong , Peggy G. Lemaux , Bob B. Buchanan
发明人： Myeong-Je Cho , Joshua H. Wong , Peggy G. Lemaux , Bob B. Buchanan
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A21D13/064 , A23J1/12 , A23K10/30 , A23L33/105 , A23V2002/00 , C07K14/415 , C12N9/0036 , C12N15/8242 , C12N15/8245 , C12N15/8251 , C12N15/8261 , Y02A40/146 , A23V2300/21
摘要： The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.
摘要翻译：本发明涉及一种转基因植物，其中所述植物的至少一部分包括具有启动子活性的启动子的重组核酸，其与编码硫氧还蛋白多肽的核酸可操作地连接，其中所述启动子是种子或谷物成熟特异性启动子和硫氧还蛋白多肽包括氨基酸序列WCGPC。本发明进一步涉及在种子中过表达硫氧还蛋白的转基因植物，其中硫氧还蛋白的过表达h与种子的白蛋白部分的蛋白质的降低（-SH）相比显着增加。

5. 发明授权

US07157629B2 Production of proteins in plant seeds 有权
标题翻译：在植物种子中生产蛋白质
公开(公告)号：US07157629B2
公开(公告)日：2007-01-02
申请号：US10461634
申请日：2003-06-13
申请人： Myeong-Je Cho , Peggy G. Lemaux , Bob B. Buchanan
发明人： Myeong-Je Cho , Peggy G. Lemaux , Bob B. Buchanan
IPC分类号： C12N15/09 , C12N15/82 , C12N15/00 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8257 , C12N15/8201 , C12N15/8207 , C12N15/8216 , C12N15/8221 , C12N15/8222 , C12N15/8234 , C12N15/8242
摘要： Transgenic monocot plants and seeds comprising monocot seed-specific promoters from seed-storage protein genes and monocot seed-specific targets sequences of expression of heterologous proteins in subcellular compartments to protect the protein are disclosed. The transgenic monocot plants include maize, wheat, rice and barley. Also disclosed are methods of producing transgenic monocot seed from the transgenic plants.
摘要翻译：公开了转基因单子叶植物和种子，其包含来自种子储存蛋白基因的单子叶种子特异性启动子和单子叶种子特异性靶向亚细胞区室中异源蛋白质表达序列以保护蛋白质。转基因单子叶植物包括玉米，小麦，稻和大麦。还公开了从转基因植物产生转基因单子叶种子的方法。

6. 发明授权

US06833493B2 Barley gene for thioredoxin and NADP-thioredoxin reductase 失效
标题翻译：用于硫氧还蛋白和NADP-硫氧还蛋白还原酶的大麦基因
公开(公告)号：US06833493B2
公开(公告)日：2004-12-21
申请号：US10091841
申请日：2002-03-05
申请人： Myeong-Je Cho , Gregorio del Val , Maxime Caillau , Peggy G. Lemaux , Bob B. Buchanan
发明人： Myeong-Je Cho , Gregorio del Val , Maxime Caillau , Peggy G. Lemaux , Bob B. Buchanan
IPC分类号： A01H900
CPC分类号： C12N15/8251 , A21D13/064 , A23J1/12 , A23L33/105 , A23V2002/00 , C07K14/415 , C12N9/0036 , C12N15/8242 , C12N15/8245 , C12N15/8261 , Y02A40/146 , A23V2300/21
摘要： The present invention provides barley thioredoxin h nucleic acids and NADP-thioredoxin reductase nucleic acids, the respective encoded proteins and methods of use.
摘要翻译：本发明提供大麦硫氧还蛋白h核酸和NADP-硫氧还蛋白还原酶核酸，各自编码的蛋白质和使用方法。

7. 发明授权

US06677433B2 Stabilization of hypoallergenic, hyperdigestible previously reduced proteins 失效
标题翻译：稳定低变应原，超可消化的先前减少的蛋白质
公开(公告)号：US06677433B2
公开(公告)日：2004-01-13
申请号：US09779375
申请日：2001-02-07
申请人： Bob B. Buchanan , Susumu Morigasaki , Gregorio del Val , Oscar L. Frick
发明人： Bob B. Buchanan , Susumu Morigasaki , Gregorio del Val , Oscar L. Frick
IPC分类号： C07K1400
CPC分类号： A21D2/26 , A21D2/264 , A21D2/265 , A21D2/266 , A21D2/36 , A21D8/04 , A21D13/064 , A23C9/1209 , A23C9/1213 , A23C9/152 , A23J1/12 , A23J1/14 , A23J3/08 , A23J3/18 , A23K20/147 , A23L5/27 , A23L7/109 , A23L11/34 , A23L13/48 , A23L33/17 , A61K38/00 , A61K39/35 , A61K2035/122 , C07K1/1133 , C07K14/415 , C07K14/43504 , C07K14/46 , C07K14/76 , C07K14/811 , C12N9/0036 , C12N9/20 , C12P21/02 , C12Q1/26 , C12Q1/34 , C12Y108/01008 , C12Y108/01009
摘要： Disulfide proteins showed mitigated allergenicity and increased digestibility by pepsin following reduction by thioredoxin. The sulfhydryl groups newly formed on reduction by thioredoxin (at 4° C.) or dithiothreitol (DTT) (at 55° C.) were blocked with a physiological disulfide, such as cystamine or oxidized glutathione (GSSG) to obtain stable forms of the disarmed allergen. When derivatized with cystamine, BLG was separated from its oxidized and reduced forms on non-reducing SDS-PAGE and appeared to lack sulfhydryl groups. Although less effective GSSG, gave similar results. Allergenicity of the two derivatives was compared with that of the oxidized, reduced and reoxidized forms of BLG by skin testing dogs from a colony sensitized to cow's milk. Both the cystamine and GSSG derivatized BLG showed decreased allergenicity and increased sensitivity to pepsin as compared to controls. The reoxidized form resembled the derivatives in having lower allergenicity. The thioredoxin- and DTT-reduced forms showed hypoallergenic, hyperdigestible properties, most effectively when the reduced proteins were heated at 55° C. Whole milk subjected to these procedures showed results similar to those obtained with pure BLG. Other proteins are similarly stabilized. Stable forms of such disarmed, hypoallergenic and hyperdigestible disulfide protein allergens or just hypoallergenic or just hyperdigestible protein allergens are useful in foods as well as clinical preparations.
摘要翻译：二硫化物蛋白质在硫氧还蛋白还原后显示减轻的过敏原性和胃蛋白酶的消化率增加。通过硫氧还蛋白（4℃）还原二硫苏糖醇（DTT）（在55℃）下新形成的巯基用生理二硫化物如胱氨酸或氧化型谷胱甘肽（GSSG）封闭，得到稳定形式的解除武装的过敏原当用胱胺衍生化时，BLG在非还原性SDS-PAGE上从其氧化和还原形式分离出来，并且似乎缺少巯基。虽然GSSG效果较差，但得到了类似的结果。将两种衍生物的过敏原与来自对牛奶敏感的菌落进行皮肤测试的狗的氧化，还原和再氧化形式的BLG进行比较。与对照相比，胱氨酸和GSSG衍生的BLG都显示出降低的过敏原性和对胃蛋白酶的敏感性增加。再氧化形式类似于具有较低过敏原性的衍生物。当减少的蛋白质在55℃下加热时，硫氧还蛋白和DTT减少的形式显示出低变应原性，超消化性质，最有效的是，经过这些程序的全脂乳显示出与用纯BLG获得的结果相似的结果。其他蛋白质同样稳定。这种解除武装，低变应原和超可消化的二硫化物蛋白质过敏原或刚刚低变应原或仅仅是超可消化的蛋白质过敏原的稳定形式可用于食物以及临床制剂。

8. 发明授权

US06555116B1 Alleviation of the allergenic potential of airborne and contact allergens by thioredoxin 失效
标题翻译：通过硫氧还蛋白降低机载和接触过敏原的过敏潜能
公开(公告)号：US06555116B1
公开(公告)日：2003-04-29
申请号：US09238379
申请日：1999-01-27
申请人： Bob B. Buchanan , Gregorio del Val , Rosa M. Lozano , Joshua H. Wong , Boihon C. Yee , Oscar L. Frick
发明人： Bob B. Buchanan , Gregorio del Val , Rosa M. Lozano , Joshua H. Wong , Boihon C. Yee , Oscar L. Frick
IPC分类号： A61K3936
CPC分类号： A21D2/26 , A21D2/264 , A21D2/265 , A21D2/266 , A21D2/36 , A21D8/04 , A21D13/064 , A23C9/1209 , A23C9/1213 , A23C9/152 , A23J1/12 , A23J1/14 , A23J3/08 , A23J3/18 , A23K20/147 , A23L5/27 , A23L7/109 , A23L11/34 , A23L13/48 , A23L33/17 , A61K39/35 , A61K2035/122 , C07K1/1133 , C07K14/415 , C07K14/43504 , C07K14/46 , C07K14/76 , C07K14/811 , C12N9/0036 , C12N9/20 , C12P21/02 , C12Q1/26 , C12Q1/34 , C12Y108/01008 , C12Y108/01009 , Y10S436/904 , Y10T436/182
摘要： Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.
摘要翻译：硫氧还蛋白，一种小二硫醇蛋白，是一种特异性的还原剂，适用于过敏原蛋白，特别是花粉和动植物来源的变应原蛋白。所有靶向蛋白含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。蛋白质是过敏性活性的，在氧化（S-S）状态下消化不良。当减少（SH状态）时，它们失去过敏原性和/或变得更易消化。当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或硫辛酸化学还原剂被活化（还原）时，硫氧还蛋白实现了这一还原。用致敏狗进行的皮肤试验表明，在注射前用降低的硫氧还蛋白处理花粉消除或降低了花粉的致敏性。研究显示硫氧还蛋白还原后，通过胃蛋白酶消化花粉蛋白。通过硫氧还蛋白还原的花粉蛋白是有效和安全的免疫治疗剂，用于减少或消除否则在暴露于未还原花粉蛋白时会发生的动物过敏反应。

9. 发明授权

US6113951A Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins 失效
公开(公告)号：US6113951A
公开(公告)日：2000-09-05
申请号：US211673
申请日：1994-11-21
申请人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee , Joshua H. Wong , Rosa Lozano , Jin-an Jiao , Sungho Shin
发明人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee , Joshua H. Wong , Rosa Lozano , Jin-an Jiao , Sungho Shin
IPC分类号： A23L1/172 , A21D2/26 , A21D8/04 , A21D10/00 , A23C9/12 , A23J1/12 , A23J1/14 , A23J3/18 , A23L1/015 , A23L1/16 , A23L1/18 , A23L1/182 , A23L1/211 , A23L1/314 , A61K35/58 , A61K35/64 , A61K38/44 , A61P39/02 , C07K1/113 , C07K14/415 , C07K14/435 , C07K14/46 , C07K14/76 , C07K14/81 , C12N1/19 , C12N9/02 , C12N9/20 , C12N9/96 , C12N9/99 , C12N11/02 , C12P21/02 , A21D13/06 , A23J3/14 , C12P21/06
CPC分类号： C12P21/02 , A21D2/26 , A21D2/265 , A21D2/266 , A21D8/04 , A23C9/1213 , A23J1/12 , A23J1/14 , A23J3/18 , A23L11/34 , A23L13/48 , A23L5/27 , A23L7/109 , A23L7/196 , C07K1/1133 , C07K14/415 , C07K14/46 , C07K14/76 , C07K14/811 , C12N9/0036 , C12N9/20 , C12Y108/01008 , C12Y108/01009 , A61K38/00
摘要： Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase). When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated; thioredoxin reduction of this protein destroyed or greatly reduced its inhibitory activity.

10. 发明授权

US06610334B1 Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins 失效
标题翻译：使用硫醇氧化还原蛋白来减少蛋白质分子内二硫键，用于提高谷物，面团和烘焙食品的质量，以及灭活蛇，蜜蜂和蝎毒素
公开(公告)号：US06610334B1
公开(公告)日：2003-08-26
申请号：US09447615
申请日：1999-11-23
申请人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee
发明人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee
IPC分类号： A21D1000
CPC分类号： C12P21/02 , A21D2/26 , A21D2/265 , A21D2/266 , A21D8/04 , A23C9/1213 , A23J1/12 , A23J1/14 , A23J3/18 , A23L5/27 , A23L7/109 , A23L7/196 , A23L11/34 , A23L13/48 , A61K38/00 , C07K1/1133 , C07K14/415 , C07K14/46 , C07K14/76 , C07K14/811 , C12N9/0036 , C12N9/20 , C12Y108/01008 , C12Y108/01009 , Y02A50/473 , A61K38/44 , A61K2300/00
摘要： Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or E. coli. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.
摘要翻译：提供了减少含有动物和植物蛋白质的方法，以及改善面团和焙烤食品的特性，包括将生面团成分与硫醇氧化还原蛋白混合以形成面团并烘焙面团以形成烤制品的步骤。本发明的方法优选使用减少的硫氧还蛋白与小麦粉，其赋予更强的面团和更高的面包体积。还提供了用硫醇氧化还原（SH）试剂降低蛇，蜂和蝎毒素蛋白，从而使个体灭活蛋白或使蛋白质解毒的方法。包括大豆的Kunitz和Bowman-Birk胰蛋白酶抑制剂的蛋白酶抑制剂也被来自大肠杆菌或小麦胚芽的NADP /硫氧还蛋白体系（NADPH，硫氧还蛋白和NADP-硫氧还蛋白还原酶）还原。当被硫氧还蛋白减少时，Kunitz和Bowman-Birk大豆胰蛋白酶抑制剂失去了抑制胰蛋白酶的能力。此外，还原形式的抑制剂显示由发芽小麦种子中的枯草杆菌蛋白酶或蛋白酶制剂对热和蛋白水解的敏感性增加。蓖麻种子胚乳的2S白蛋白由小麦胚芽或大肠杆菌的硫氧还蛋白降低。硫氧还蛋白被NADPH和NADP硫氧还蛋白还原酶或二硫苏糖醇还原。分析表明，硫氧还蛋白活性降低了2S大亚基的分子内二硫化物，但在减少连接大亚基与小亚基的分子间二硫化物方面无效。分离出含有蛋白质的新型胱氨酸，其抑制支链淀粉酶。蛋白质被硫氧还蛋白还原，当其还原时，其抑制活性被破坏或大大降低。

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