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1. 发明授权

US4147120A Auxiliary feed mechanism for sewing machines 失效
标题翻译：缝纫机辅助进给机构
公开(公告)号：US4147120A
公开(公告)日：1979-04-03
申请号：US833110
申请日：1977-09-14
申请人： Maximilian Adamski, Jr. , Robert C. Talsma , Richard E. Walters
发明人： Maximilian Adamski, Jr. , Robert C. Talsma , Richard E. Walters
IPC分类号： D05B27/12 , D05B21/00
CPC分类号： D05B27/12 , D05D2207/02
摘要： An auxiliary feed mechanism adapted for use with a sewing machine including a feed roller for advancing the workpiece through the work station and a mounting structure adapted to move the feed roller between two positions in one of which the feed roller is operative to aid in feeding the workpiece through the workstation and in the other of which the feed roller is rendered ineffective to transmit motive power to the workpiece. A plurality of flexible couplings drive the feed roller in timed relation to the actuation of a variable speed drive motor which is coupled in a unique electric circuit relationship with the main drive motor of the machine so as to vary the feeding rate of the feed roller in accordance with the machine cycle rate.
摘要翻译：一种适于与缝纫机一起使用的辅助进给机构，该缝纫机包括用于使工件通过工作台前进的进给辊和适于将进给辊移动到两个位置之间的安装结构，其中一个进给辊可操作以帮助进给工件通过工作站，另一个工件使进给辊无效地将动力传递到工件。多个柔性联轴器以与驱动机构的驱动电动机独特的电路关系的变速驱动电动机的动作的定时关系驱动进给辊，以便改变进给辊的进给速度按照机器循环速率。

2. 发明授权

US06916656B2 Non-linear amplitude dielectrophoresis waveform for cell fusion 有权
标题翻译：用于细胞融合的非线性幅度介电电泳波形
公开(公告)号：US06916656B2
公开(公告)日：2005-07-12
申请号：US10487235
申请日：2002-04-05
申请人： Richard E. Walters , Derin C. Walters , Alan D. King
发明人： Richard E. Walters , Derin C. Walters , Alan D. King
IPC分类号： C12N15/02 , C12N5/10 , C12N5/16 , C12N13/00 , C12N15/64 , C12N15/74 , C12N15/87
CPC分类号： C12M35/02 , A61K2039/5152 , A61K2039/5154 , C12N5/16 , C12N13/00 , H01L2224/83856 , H01L2924/01019
摘要： An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior to being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linear algorithms.
摘要翻译：本发明的一个目的是提供一种在使生物细胞经历细胞融合脉冲之前处理生物细胞的方法，其包括用以非线性方式改变幅度的融合前电场波形来处理生物细胞的步骤，相对于时间，使得生物细胞首先用相对低幅度，长持续时间的预融合电场波形对准，然后用相对较高幅度，短持续时间的预融合电场波形压缩，导致增加的细胞膜接触先进行细胞融合。非线性预融合电场波形可以以连续的方式以S形方式以相邻步骤中逐步增加的波形以逐步增加的波形在非相邻步骤中逐步增加，并以按照非线性算法。

3. 发明申请

US20110282265A1 METHOD AND APPARATUS FOR THE DELIVERY OF PLYNUCLEOTIDE VACCINES TO MAMMALIA SKIN 审中-公开
标题翻译：将灭草鼠疫苗递送给妈妈皮肤的方法和装置
公开(公告)号：US20110282265A1
公开(公告)日：2011-11-17
申请号：US12451536
申请日：2008-05-20
申请人： Richard E. Walters , Derin C. Walters , Alan D. King , Anna-Karin Roos
发明人： Richard E. Walters , Derin C. Walters , Alan D. King , Anna-Karin Roos
IPC分类号： A61M37/00
CPC分类号： A61N1/327 , A61K39/12 , A61K2039/53 , A61K2039/54 , A61K2039/70 , A61N1/306 , C07K14/005 , C12N2770/24122 , C12N2770/24134
摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method includes administering a polynucleotide vaccine into the skin at an administration site, (b) applying a needle electrode to the skin in the vicinity to the administration site, and (c) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the 15 polynucleotide vaccine into the skin cells by electroporation.
摘要翻译：本发明的目的是提供一种用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中以增加T细胞应答并减少由于长电波形应用引起的疼痛和不适以及由于肌肉收缩而引起的不适的方法和装置。该方法包括在给药部位向皮肤施用多核苷酸疫苗，（b）将针电极施用于给药部位附近的皮肤，以及（c）施用至少三个单独的，操作者控制的，具有脉冲间隔小于100毫秒的独立编程的窄间隔电波形，以通过电穿孔将15个多核苷酸疫苗递送到皮肤细胞中。

4. 发明授权

US6010613A Method of treating materials with pulsed electrical fields 失效
公开(公告)号：US6010613A
公开(公告)日：2000-01-04
申请号：US569664
申请日：1995-12-08
申请人： Richard E. Walters , Alan D. King
发明人： Richard E. Walters , Alan D. King
IPC分类号： C12N13/00
CPC分类号： C12N13/00 , C12M35/02
摘要： A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.

5. 发明授权

US06713291B2 Electrodes coated with treating agent and uses thereof 有权
公开(公告)号：US06713291B2
公开(公告)日：2004-03-30
申请号：US09920861
申请日：2001-08-03
申请人： Alan D. King , Richard E. Walters
发明人： Alan D. King , Richard E. Walters
IPC分类号： C12N1300
CPC分类号： A61N1/325 , A61H39/002 , A61N1/306
摘要： An object of the invention is to provide a method for delivery of macromolecules into biological cells in the tissues of a patient and includes the steps of: (a) providing electrodes (16) in an electrode assembly (12), wherein the electrodes have fixed electrode surfaces (42) which are coated with at least one static layer of electrode releasable molecules (44) to be delivered; (b) providing a waveform generator (15) for generating electric fields; (c) establishing electrically conductive pathways between the electrodes (16) and the waveform generator (15); (d) locating the electrodes (16) such that the biological cells are situated therebetween, and (g) providing electric fields in the form of pulse waveforms from the waveform generator (15) to the electrodes (16), such that molecules in the at least one static layer of the electrode releasable molecules (44) on the electrodes (16) are delivered into the biological cells. The electrode releasable molecules (44) can be either electric field separable molecules and/or solvent separable material. Another object of the invention is to provide an apparatus for carrying out the method of the invention. The static-coated electrode assembly (12) can be provided in a sterile package (24), from which the electrode assembly (12) is removed prior to use. The statically-coated electrode assembly (12) can be in a form of a disposable assembly (12) which is removable and replaceable from an electrode assembly holder (13).

6. 发明授权

US06653114B2 Method and apparatus for treating materials with electrical fields having varying orientations 失效
公开(公告)号：US06653114B2
公开(公告)日：2003-11-25
申请号：US10265133
申请日：2002-10-07
申请人： Richard E. Walters , Alan D. King , Derin C. Walters
发明人： Richard E. Walters , Alan D. King , Derin C. Walters
IPC分类号： C12N1300
CPC分类号： C12N13/00 , C12M35/02
摘要： The object of the invention is to provide a method and apparatus for treating membrane-containing material with electrical fields, in vitro, and with an added treating substance. With the method, a plurality of electrodes are arrayed around the material to be treated and are connected to outputs of an electrode selection apparatus. Inputs of the electrode selection apparatus are connected to outputs of a pulse sequence generator. A treating substance is added to the membrane-containing material. Electrical pulses are applied to the electrode selection apparatus and are routed through the electrode selection apparatus in a predetermined, computer-controlled sequence to selected electrodes in the array of electrodes, whereby the membrane-containing material is treated with the added treating substance and with electrical fields. The routing of applied pulses through the electrode selection apparatus to selected electrodes can be done in an enormous number of ways.

7. 发明授权

US6078490A Method of treating materials with pulsed electrical fields 有权
标题翻译：用脉冲电场处理材料的方法
公开(公告)号：US6078490A
公开(公告)日：2000-06-20
申请号：US313260
申请日：1999-05-18
申请人： Richard E. Walters
发明人： Richard E. Walters
IPC分类号： C12N13/00 , H02H3/14
CPC分类号： C12N13/00 , C12M35/02
摘要： A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.
摘要翻译：提供了一种利用脉冲电场处理材料，特别是有机材料的方法，其中该方法包括将具有至少三个脉冲的敏捷脉冲序列应用于材料的步骤，其中敏捷脉冲序列具有一个，两个或三个具有以下特征：（1）所述至少三个脉冲中的至少两个脉冲在脉冲幅度上彼此不同; （2）至少三个脉冲中的至少两个脉冲在脉冲宽度上彼此不同; 和（3）至少三个脉冲中的第二组的第一组的第一脉冲间隔对于所述至少三个脉冲中的两个的第二组的第二脉冲间隔是不同的。当在电穿孔方法中处理生物细胞以形成孔时，诱导的孔被维持相当长的时间段，并且维持生物细胞的存活力。

8. 发明申请

US20110166501A1 Method and apparatus for the delivery of polynucleotide vaccines to mammalian skin 审中-公开
标题翻译：用于将哺乳动物皮肤递送多核苷酸疫苗的方法和装置
公开(公告)号：US20110166501A1
公开(公告)日：2011-07-07
申请号：US13065135
申请日：2011-03-15
申请人： Richard E. Walters , Derin C. Walters , Alan D. King , Anna-Karin Roos
发明人： Richard E. Walters , Derin C. Walters , Alan D. King , Anna-Karin Roos
IPC分类号： A61M37/00
CPC分类号： A61N1/327 , A61K39/12 , A61K2039/53 , A61K2039/54 , A61K2039/70 , A61N1/306 , C07K14/005 , C12N2770/24122 , C12N2770/24134
摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and to reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method for the delivery of polynucleotide vaccines into mammalian skin cells includes the steps of: (a.) administering a polynucleotide vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity to the administration site, and (c.) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the polynucleotide vaccine into the skin cells by electroporation. The sequence of at least three waveforms has one, two, or three of the following characteristics (1) at least two of the at least three waveforms differ from each other in waveform amplitude, (2) at least two of the at least three waveforms differ from each other in waveform width, and (3) a first waveform interval for a first set of two of the at least three waveforms is different from a second waveform interval for a second set of two of the at least three waveforms.
摘要翻译：本发明的目的是提供一种用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中以增加T细胞应答并减少由于长电波形应用引起的疼痛和不适以及由于肌肉收缩引起的疼痛和不适的方法和装置。用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中的方法包括以下步骤：（a）在给药部位将多核苷酸疫苗施用于皮肤，（b）将针电极施用于给药的附近的皮肤站点，以及（c）应用脉冲间隔小于100毫秒的至少三个单个，操作者控制的，独立编程的窄间隔电波形序列，以通过电穿孔将多核苷酸疫苗递送到皮肤细胞中。至少三个波形的序列具有以下特征（1）中的一个，两个或三个，所述至少三个波形中的至少两个波形在波形幅度上彼此不同，（2）至少三个波形中的至少两个波形在波形宽度上彼此不同，以及（3）至少三个波形中的两个的第一组的第一波形间隔与用于至少三个波形中的两个的第二组的第二波形间隔不同。

9. 发明授权

US07371561B2 In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation 有权
标题翻译：体外，多电极对阵列和用于电穿孔的多处理细胞装置
公开(公告)号：US07371561B2
公开(公告)日：2008-05-13
申请号：US10694345
申请日：2003-10-28
申请人： Richard E. Walters , Paul K. Gustavson
发明人： Richard E. Walters , Paul K. Gustavson
IPC分类号： C12M1/42
CPC分类号： C12M35/02 , B01L3/5085 , B01L2300/0829 , B01L2400/0415 , C12M23/12
摘要： A multiple electrode pair array apparatus is provided for use with a multiple well plate which has multiple wells distributed in a two-dimensional matrix array which has R rows and C columns. The multiple electrode pair array apparatus includes a non-conductive base member. An array of pairs of electrodes are attached to the base member and project therefrom. The pairs of electrodes are distributed on the base member in a two-dimensional matrix array which has R rows and C columns to enable registration with the two-dimensional matrix array of wells. Each pair of electrodes includes a respective first electrode and a respective second electrode. An array of R row conductors are electrically connected to corresponding first electrodes. An array of C column conductors are oriented perpendicular to the R row conductors, are electrically insulated from the row conductors, and are electrically connected to corresponding second electrodes.
摘要翻译：提供多电极对阵列装置用于多孔板，多孔板具有分布在具有R行和C列的二维矩阵阵列中的多个阱。多电极对阵列装置包括非导电基底部件。一对电极被附接到基底构件并从其突出。电极对以具有R行和C列的二维矩阵阵列分布在基座上，以使得能够与孔的二维矩阵阵列配准。每对电极包括相应的第一电极和相应的第二电极。 R排导体的阵列电连接到对应的第一电极。 C列导体的阵列垂直于R行导体定向，与行导体电绝缘，并且电连接到对应的第二电极。

10. 发明授权

US06878538B1 Apparatus for receiving and aligning a multiple electrode pair array and a multiple treatment cell for use in electroporation 有权
标题翻译：用于接收和对准多个电极对阵列和用于电穿孔的多重处理电池的装置
公开(公告)号：US06878538B1
公开(公告)日：2005-04-12
申请号：US10694344
申请日：2003-10-28
申请人： Richard E. Walters , Benjamin R. Lane , Michael S. Abbott
发明人： Richard E. Walters , Benjamin R. Lane , Michael S. Abbott
IPC分类号： B01L9/00 , C12M1/42 , C12M3/00 , C12N20060101
CPC分类号： B01L9/523 , B01L2300/046 , B01L2300/0829 , C12M23/12 , C12M23/48 , C12M35/02 , Y10S435/809
摘要： An apparatus is provided for receiving and aligning a multiple electrode pair array and a multiple well plate used in electroporation. The apparatus includes a housing which includes a bottom housing portion and a pair of side housing portions which project upward from the bottom housing portion. A pair of board-reception structures are adjacent to the inner portions of the side housing portions. Multiple well plate engaging and disengaging means, supported by the housing, are provided for engaging a multiple well plate with a multiple electrode pair array and for disengaging a multiple well plate from a multiple electrode pair array. Preferably, the multiple well plate engaging and disengaging means include a multiple well plate lifting and lowering assembly. The multiple well plate lifting and lowering assembly can include lifting/lowering handles located outside the housing.
摘要翻译：提供了用于接收和对准多电极对阵列和用于电穿孔的多孔板的装置。该装置包括壳体，该壳体包括底部壳体部分和从底部壳体部分向上突出的一对侧部壳体部分。一对板接收结构邻近侧壳体部分的内部部分。提供由壳体支撑的多个孔板接合和分离装置，用于使多孔板与多个电极对阵列接合并且用于使多个孔板与多个电极对阵列分离。优选地，多孔板接合和分离装置包括多孔板升降组件。多孔板升降组件可以包括位于壳体外部的升降手柄。

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