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1. 发明授权

US08859226B2 Measurement method for viable cell count, and culture medium 有权
标题翻译：活细胞计数的测定方法和培养基
公开(公告)号：US08859226B2
公开(公告)日：2014-10-14
申请号：US13390859
申请日：2011-03-25
申请人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
发明人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
IPC分类号： C12N1/20 , C12Q1/06 , C12Q1/04
CPC分类号： C12Q1/045 , C12N1/20 , C12Q1/06 , G01N2333/36
摘要： There are provided a method of identifying the longum species alone, such as Bifidobacterium longum subsp. longum alone, and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains L-arabinose as a sole sugar source; and also a culture medium which is useful as a selective medium for the above measurement method and which is also easy to prepare.
摘要翻译：提供了一种识别长单独物种的方法，例如长双歧杆菌（Bifidobacterium longum subsp。）并且通过使用含有L-阿拉伯糖作为唯一糖源的培养基，测量其中包含属于双歧杆菌属的微生物的待测试细菌细胞中的活细胞数; 以及作为上述测定方法的选择培养基有用的培养基，也容易制备。

2. 发明申请

US20120244571A1 MEASUREMENT METHOD FOR VIABLE CELL COUNT, AND CULTURE MEDIUM 审中-公开
标题翻译：可视电池计数和文化媒质的测量方法
公开(公告)号：US20120244571A1
公开(公告)日：2012-09-27
申请号：US13391855
申请日：2011-03-25
申请人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
发明人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
IPC分类号： C12Q1/06
CPC分类号： C12Q1/06 , C12N1/20 , C12Q1/045 , G01N2333/195
摘要： There are provided a method of identifying a microorganism belonging to Bifidobacterium breve alone and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains at least one sugar alcohol selected from sorbitol and mannitol as a sole sugar source; and also a culture medium which is useful as a selective medium to be used in the above measurement method and which is also easy to prepare.
摘要翻译：提供一种通过使用含有至少一种糖醇的培养基来鉴定属于双歧杆菌的微生物在测定的细菌细胞中测定活细胞数的方法，所述细菌细胞包括双歧杆菌属的微生物选自山梨糖醇和甘露醇作为唯一的糖源; 以及作为上述测定方法中使用的也易于制备的选择性培养基的培养基。

3. 发明授权

US09014990B2 Device for estimating survival cell count, computer program, and recording medium 有权
标题翻译：用于估计存活细胞计数，计算机程序和记录介质的装置
公开(公告)号：US09014990B2
公开(公告)日：2015-04-21
申请号：US12995066
申请日：2009-05-29
申请人： Fumiaki Abe , Ayako Uchijima , Masamichi Muto , Ayako Horigome
发明人： Fumiaki Abe , Ayako Uchijima , Masamichi Muto , Ayako Horigome
IPC分类号： G01N31/00 , C12Q1/06
CPC分类号： C12Q1/06
摘要： By accurately estimating the survival cell count in a probiotic product, the time for developing the product can be shortened. In a device (1) for estimating survival cell count, a calculation unit (14) calculates an estimated result of the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10nt=Log10n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)× 1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.
摘要翻译：通过准确估计益生菌产品中的存活细胞计数，可以缩短开发产品的时间。在用于估计存活细胞计数的装置（1）中，计算单元（14）根据以下等式计算储存后的组合物中包含的特定菌株的存活细胞计数nt（CFU / g）的估计结果（一世）。（I）Log10nt = Log10n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表储存期（天）×1/30，nt表示存活细胞在储存期t（天）后，组合物中所含的菌株的计数（CFU / g），n0表示开始保存时组合物中包含的菌株的活菌数（CFU / g），T表示储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的应变特性系数，BT和DT代表对应变特异的实验测定常数。

4. 发明申请

US20110071768A1 DEVICE FOR ESTIMATING SURVIVAL CELL COUNT, COMPUTER PROGRAM, AND RECORDING MEDIUM 有权
标题翻译：用于估计生存细胞计数，计算机程序和记录介质的设备
公开(公告)号：US20110071768A1
公开(公告)日：2011-03-24
申请号：US12995066
申请日：2009-05-29
申请人： Fumiaki Abe , Ayako Uchijima , Masamichi Muto , Ayako Horigome
发明人： Fumiaki Abe , Ayako Uchijima , Masamichi Muto , Ayako Horigome
IPC分类号： G06F19/00 , G01N33/48
CPC分类号： C12Q1/06
摘要： By accurately estimating the survival cell count in a probiotic product, the time for developing the product can be shortened. In a device (1) for estimating survival cell count, a calculation unit (14) calculates an estimated result of the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)× 1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.
摘要翻译：通过准确估计益生菌产品中的存活细胞计数，可以缩短开发产品的时间。在用于估计存活细胞计数的装置（1）中，计算单元（14）根据以下等式计算储存后的组合物中包含的特定菌株的存活细胞计数nt（CFU / g）的估计结果（一世）。（I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示储存期t（天）后组合物中所含的菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

5. 发明申请

US20110104746A1 Diluting Solution For Suspension For Use In The Measurement Of Number Of Living Microbial Cells Contained In Sample, And Method For Measurement Of Number Of Living Microbial Cells 审中-公开
标题翻译：用于悬浮液的稀释溶液用于测量样品中包含的活细菌细胞数量，以及用于测量活细菌细胞数量的方法
公开(公告)号：US20110104746A1
公开(公告)日：2011-05-05
申请号：US13000102
申请日：2009-06-11
申请人： Masamichi Muto
发明人： Masamichi Muto
IPC分类号： C12Q1/06
CPC分类号： C12Q1/06
摘要： A dilution buffer for suspension and an enumeration method, which, for the measurement of the viable cell count of a microorganism contained within a sample, and particularly a powdered product such as a milk powder, yield more accurate measurement values than have conventionally been obtainable. A dilution buffer for suspension, used for preparing a suspension buffer of a sample when conducting a measurement of the viable cell count of a microorganism contained within the sample, wherein the dilution buffer contains polysorbates at a concentration of not less than 0.5%. Also, an enumeration method for a microorganism contained within a sample, the method including: preparing a suspension buffer of the sample using a dilution buffer for suspension containing polysorbates at a concentration of not less than 0.5%, and measuring the viable cell count of the microorganism contained within the suspension buffer.
摘要翻译：用于悬浮液的稀释缓冲液和计数方法，其用于测量样品中包含的微生物，特别是粉末产品如奶粉的活细胞计数，产生比常规可获得的更准确的测量值。一种用于悬浮液的稀释缓冲液，用于在进行样品中包含的微生物的活细胞计数的测量时制备样品的悬浮液缓冲液，其中所述稀释缓冲液含有浓度不小于0.5％的聚山梨醇酯。另外，对于样本中含有的微生物的计数方法，其特征在于，所述方法包括：使用浓度为0.5％以上的含有聚山梨酸酯的悬浮液的稀释缓冲液制备所述样品的悬浮液缓冲液，包含在悬浮液缓冲液中的微生物。

6. 发明授权

US5759844A Antibacterial articles and methods of producing the articles 失效
标题翻译：抗菌制品和制品的制造方法
公开(公告)号：US5759844A
公开(公告)日：1998-06-02
申请号：US568381
申请日：1995-12-06
申请人： Jun Hiraki , Sumiko Watanabe , Masamichi Muto
发明人： Jun Hiraki , Sumiko Watanabe , Masamichi Muto
IPC分类号： A01N25/02 , A01N25/10 , A01N37/20 , A01N63/02 , C12P21/02 , C12N1/06
CPC分类号： C12P21/02
摘要： An antibacterial article inhibits the proliferation of microorganisms on the surface of food. The antibacterial article includes a substrate, such as a nonwoven material, on which is adsorbed .epsilon.-polylysine. The article is produced by applying an aqueous or alcoholic solution of .epsilon.-polylysine to the substrate by spraying or immersion.
摘要翻译：抗菌制品抑制食物表面微生物的增殖。抗菌制品包括其上吸附了ε-聚赖氨酸的基底，例如非织造材料。通过喷雾或浸渍将ε-聚赖氨酸的水溶液或醇溶液施用于基材来制备制品。

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