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    • 1. 发明申请
    • TWO-DIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY
    • 用于荧光显微镜的双向扫描
    • US20150144806A1
    • 2015-05-28
    • US14401103
    • 2013-05-28
    • Macquarie University
    • Dayong JinYiqing LuJames Austin Piper
    • G01N21/64
    • G01N21/6458G01N15/1456G01N21/6408G01N21/6486G01N2201/10G02B21/16
    • In one form, a two-directional scanning method for luminescence microscopy is disclosed. A series of continuous scans are performed by an interrogation wide-field relative to a first direction and a target is identified. A precise position of the target is determined in the first direction. At least one scan by the interrogation wide-field is performed relative to a second direction at or near the precise position of the target in the first direction. The two-directional scanning method produces “on-the-fly” (i.e. ex tempore or impromptu) precise localization of targets. Embodiments open up new applications for background-free or background-reduced luminescence microscopy, for example time-gated or time-resolved luminescence microscopy, in a relatively fast, higher speed or more efficient manner.
    • 在一种形式中,公开了用于发光显微镜的双向扫描方法。 通过相对于第一方向的询问广域来执行一系列连续扫描,并且识别目标。 在第一方向上确定目标的精确位置。 相对于目标在第一方向的精确位置处或附近的第二方向,通过询问广域进行至少一次扫描。 双向扫描方法产生目标的“即时”(即临时或即兴)精确定位。 实施例以相对快速,更高速或更有效的方式开辟了无背景或背景减少发光显微镜的新应用,例如时间门控或时间分辨发光显微镜。