专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20160097091A1 SEQUENCING METHODS, COMPOSITIONS AND SYSTEMS 审中-公开
标题翻译：序列方法，组成和系统
公开(公告)号：US20160097091A1
公开(公告)日：2016-04-07
申请号：US14856220
申请日：2015-09-16
申请人： LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES GmbH
发明人： Wolfgang HINZ , Peter VANDER HORN , Earl HUBBELL , Christian WOEHLER
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2535/113 , C12Q2565/519
摘要： In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.
摘要翻译：在一些实施方案中，本公开一般涉及用于进行核苷酸掺入的方法以及组合物，系统，试剂盒和装置，其包括：（a）提供包含一个或多个含有聚合酶和核酸模板的反应位点的表面，所述反应位点已经或被杂交到可延伸的一端; （b）通过使一个或多个所述反应位点与包含一种或多种类型的终止子核苷酸的第一溶液接触来进行第一核苷酸流; （c）使用所述聚合酶在至少一个所述反应位点内的所述核酸模板的可延伸末端掺入至少一种类型的终止子核苷酸; 和（d）使用附着或可操作地连接至少一个反应位点的传感器检测表示核苷酸掺入的非光学信号。

2. 发明申请

US20180119217A1 MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开
公开(公告)号：US20180119217A1
公开(公告)日：2018-05-03
申请号：US15561445
申请日：2016-03-18
申请人： LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES GMBH
发明人： Wolfgang HINZ , Steven MENCHEN , Ronald GRAHAM , Peter VANDER HORN , Earl HUBBELL , Christian WOEHLER , Roman ROZHKOV , Barnett ROSENBLUM
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q2565/607
摘要： In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

3. 发明申请

US20210155726A1 HYDROPHILIC POLYMERIC PARTICLES AND METHODS FOR MAKING AND USING SAME 有权
公开(公告)号：US20210155726A1
公开(公告)日：2021-05-27
申请号：US17165039
申请日：2021-02-02
申请人： LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES AS
发明人： Geir FONNUM , Grete Irene MODAHL , Nini Hofsloekken KJUS , Astrid Evenroed MOLTEBERG , Diem Thuy Thi TRAN , Jo AASERUD , M. Tahla GOKMEN , Steven M. MENCHEN , Carl FULLER , Luisa ANDRUZZI , Wolfgang HINZ
IPC分类号： C08F130/08 , C07F7/18 , C08F8/12 , C12Q1/686 , C08F2/20 , C08F2/26 , C08F220/58 , C08F230/08 , C08F6/24 , C08F222/38 , C08F8/32 , C08F8/34 , C12P19/34 , C12Q1/6874 , G01N27/414 , C08J9/20
摘要： A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

4. 发明申请

US20190390018A1 POLYMER SUBSTRATES FORMED FROM CARBOXY FUNCTIONAL ACRYLAMIDE 审中-公开
公开(公告)号：US20190390018A1
公开(公告)日：2019-12-26
申请号：US16253141
申请日：2019-01-21
申请人： LIFE TECHNOLOGIES AS , LIFE TECHNOLOGIES CORPORATION
发明人： Geir FONNUM , Steven M. MENCHEN , M. Talha GOKMEN , Pontus LUNDBERG , Prasanna Krishnan THWAR , Alfred LUI , Lily LU , Wolfgang HINZ , Lene HUSABOE , Elisabeth BREIVOLD , Astrid Evenroed MOLTEBERG , Synne LARSEN
IPC分类号： C08J3/075 , C08F220/70 , C08F8/12 , C08F220/58
摘要： A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

5. 发明申请

US20190233558A1 HYDROPHILIC POLYMERIC PARTICLES AND METHODS FOR MAKING AND USING SAME 审中-公开
公开(公告)号：US20190233558A1
公开(公告)日：2019-08-01
申请号：US16259963
申请日：2019-01-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Geir FONNUM , Grete Irene MODAHL , Nini KJUS , Astrid Evenroed MOLTEBERG , Diem Thuy Thi TRAN , Jo AASERUD , Muhammed GOKMEN , Steven M. MENCHEN , Carl FULLER , Luisa ANDRUZZI , Wolfgang HINZ
IPC分类号： C08F130/08 , C07F7/18 , C08F230/08 , C08F8/32 , C08F8/34 , C08J9/20 , G01N27/414 , C12Q1/6874 , C12P19/34 , C08F222/38 , C12Q1/686 , C08F220/58 , C08F2/26 , C08F2/20 , C08F6/24 , C08F8/12
CPC分类号： C08F130/08 , C07F7/1804 , C07F7/188 , C08F2/20 , C08F2/26 , C08F6/24 , C08F8/12 , C08F8/32 , C08F8/34 , C08F220/58 , C08F222/385 , C08F230/08 , C08F2800/20 , C08J9/20 , C08J2343/04 , C12P19/34 , C12Q1/686 , C12Q1/6874 , G01N27/4145 , Y10T428/2982 , C08F257/02 , C08F2222/1013 , C08F212/36 , C08F220/60 , C12Q2563/149 , C12Q2563/159 , C12Q2565/543
摘要： A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

6. 发明申请

US20190017107A1 MATRIX ARRAYS AND METHODS FOR MAKING SAME 审中-公开
公开(公告)号：US20190017107A1
公开(公告)日：2019-01-17
申请号：US16120007
申请日：2018-08-31
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： David LIGHT , Wolfgang HINZ , Ronald CICERO , Christina INMAN , Paul KENNEY , Alexander MASTROIANNI , Roman ROZHKOV , Yufang WANG , Jeremy GRAY , Marc GLAZER , Dmitriy GREMYACHINSKIY
IPC分类号： C12Q1/6837 , C12Q1/6834 , C12Q1/6869 , C12Q1/6806
CPC分类号： C12Q1/6837 , C12Q1/6806 , C12Q1/6834 , C12Q1/6869 , C12Q2523/101 , C12Q2527/119 , C12Q2535/122 , C12Q2547/107 , C12Q2563/159 , C12Q2565/537 , C12Q2523/301 , C12Q2523/32
摘要： A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

7. 发明申请

US20180334708A1 METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 审中-公开
公开(公告)号：US20180334708A1
公开(公告)日：2018-11-22
申请号：US15949456
申请日：2018-04-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan ROTHBERG , Wolfgang HINZ , Kim JOHNSON , James BUSTILLO
IPC分类号： C12Q1/6869 , G01N27/414 , H01L27/088 , H01L23/00 , C12Q1/6874 , H01L21/306 , H01L29/78
CPC分类号： C12Q1/6869 , C12Q1/6874 , G01N27/414 , G01N27/4145 , G01N27/4148 , H01L21/306 , H01L24/18 , H01L24/20 , H01L24/82 , H01L27/088 , H01L29/78 , H01L2224/04105 , H01L2224/16 , H01L2224/76155 , H01L2224/82102 , H01L2924/01005 , H01L2924/01006 , H01L2924/01011 , H01L2924/01012 , H01L2924/01013 , H01L2924/01015 , H01L2924/01019 , H01L2924/0102 , H01L2924/01023 , H01L2924/01024 , H01L2924/01027 , H01L2924/01029 , H01L2924/0103 , H01L2924/01033 , H01L2924/01046 , H01L2924/01047 , H01L2924/01052 , H01L2924/01056 , H01L2924/01059 , H01L2924/01073 , H01L2924/01074 , H01L2924/01075 , H01L2924/01082 , H01L2924/1306 , H01L2924/13091 , H01L2924/14 , H01L2924/1433 , H01L2924/19041 , H01L2924/19042 , H01L2924/19043 , H01L2924/30105 , H01L2924/3011 , H01L2924/3025 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101 , H01L2924/00
摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

8. 发明申请

US20180179520A1 SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 审中-公开
公开(公告)号：US20180179520A1
公开(公告)日：2018-06-28
申请号：US15846195
申请日：2017-12-18
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Wolfgang HINZ , John LEAMON , David LIGHT , Jonathan M. ROTHBERG
IPC分类号： C12N15/10 , C40B50/06 , C40B40/08 , B01J19/00 , C12Q1/6874
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00466 , B01J2219/005 , B01J2219/00596 , B01J2219/00722 , C12Q1/6874 , C40B40/08 , C40B50/06 , Y10T428/2982
摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

9. 发明申请

US20170145497A1 METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 审中-公开
公开(公告)号：US20170145497A1
公开(公告)日：2017-05-25
申请号：US15276709
申请日：2016-09-26
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan M. ROTHBERG , Wolfgang HINZ , Kim L. JOHNSON , James BUSTILLO , John LEAMON , Jonathan SCHULTZ
IPC分类号： C12Q1/68 , G01N27/414 , B01L3/00
CPC分类号： C12Q1/6874 , B01L3/502761 , B01L2300/046 , B01L2300/0663 , B01L2300/0851 , B01L2400/086 , C12Q1/6869 , G01N27/4145 , G01N27/4148 , C12Q2565/607 , C12Q2565/301 , C12Q2565/629
摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

10. 发明申请

US20170029873A1 METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS 审中-公开
标题翻译：使用FET阵列检测分子相互作用的方法和装置
公开(公告)号：US20170029873A1
公开(公告)日：2017-02-02
申请号：US15192225
申请日：2016-06-24
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan M. ROTHBERG , Wolfgang HINZ
IPC分类号： C12Q1/68 , G01N27/414 , H01L29/788 , G01N33/543
CPC分类号： C12Q1/6825 , C12Q1/6869 , C12Q1/6874 , G01N27/26 , G01N27/4145 , G01N27/4148 , G01N33/54373 , G01N33/6818 , H01L29/78 , H01L29/788 , H01L2924/01006
摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.
摘要翻译：提供了与用于分析物检测和测量的大规模FET阵列相关的方法和装置。可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式