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1. 发明申请

US20100285573A1 Apparatus for processing a sample in a liquid droplet and method of using the same 有权
标题翻译：用于处理液滴中的样品的装置及其使用方法
公开(公告)号：US20100285573A1
公开(公告)日：2010-11-11
申请号：US11984197
申请日：2007-11-14
申请人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
发明人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
IPC分类号： C12M1/34
CPC分类号： G01N1/31 , B01L3/5088 , B01L2300/0609 , B01L2300/0829 , B01L2300/089 , B01L2300/161 , B01L2400/0415 , C12M25/01 , G01N33/54373 , G01N2035/1037
摘要： The invention provides an apparatus and a method of processing a biological and/or chemical sample in a liquid droplet. The apparatus comprises a processing compartment, which is defined by a reservoir and an immobilisation member. The processing compartment is further adapted to accommodate a medium, which is immiscible with the liquid droplet, and of a lower surface energy than the liquid of the liquid droplet. The reservoir is defined by a circumferential wall and a base. The immobilisation member is arranged within the reservoir and comprises a surface that is patterned in such a way that it comprises at least one predefined immobilisation area. The predefined immobilisation area within the patterned surface is of a higher surface energy than the medium. Furthermore the at least one predefined area is of a higher surface energy than the remaining surface and of a sufficient width in the plane of the surface to allow, in said hydrophobic medium, the immobilisation of the liquid droplet on the hydrophilic area via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions. The remaining surface is of at most about the same surface energy as the medium. In the method of the invention the medium is disposed into the apparatus, such that the predefined immobilisation area is entirely covered by the medium. The liquid droplet is disposed onto the predefined immobilisation area, whereby the liquid droplet is immobilised thereon via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions. A process is performed on the biological and/or chemical sample in said liquid droplet.
摘要翻译：本发明提供了一种在液滴中处理生物和/或化学样品的装置和方法。该装置包括由储存器和固定构件限定的处理室。处理室还适于容纳与液滴不混溶的介质和比液滴的液体低的表面能。储存器由圆周壁和底座限定。固定构件布置在储存器内并且包括以使得其包括至少一个预定义的固定区域的方式图案化的表面。图案化表面内的预定义的固定区域具有比介质更高的表面能。此外，至少一个预定义的区域具有比剩余表面更高的表面能并且在表面的平面中具有足够的宽度，以允许在所述疏水介质中通过亲水亲水性将液滴固定在亲水区域上或疏水 - 疏水相互作用。剩余的表面与介质的表面能最多相同。在本发明的方法中，将介质放置在设备中，使得预定义的固定区域被介质完全覆盖。将液滴设置在预定义的固定区域上，由此通过亲水亲水或疏水 - 疏水相互作用将液滴固定在其上。对所述液滴中的生物和/或化学样品进行处理。

2. 发明授权

US08691147B2 Apparatus for processing a sample in a liquid droplet and method of using the same 有权
标题翻译：用于处理液滴中的样品的装置及其使用方法
公开(公告)号：US08691147B2
公开(公告)日：2014-04-08
申请号：US11984197
申请日：2007-11-14
申请人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
发明人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
IPC分类号： G01N21/00 , G01N1/00 , G01N33/00 , B01L9/00
CPC分类号： G01N1/31 , B01L3/5088 , B01L2300/0609 , B01L2300/0829 , B01L2300/089 , B01L2300/161 , B01L2400/0415 , C12M25/01 , G01N33/54373 , G01N2035/1037
摘要： The invention provides an apparatus and a method of processing a biological and/or chemical sample in a liquid droplet. The apparatus comprises a processing compartment, which is defined by a reservoir and an immobilization member. The processing compartment is further adapted to accommodate a medium, which is immiscible with the liquid droplet, and of a lower surface energy than the liquid of the liquid droplet. The reservoir is defined by a circumferential wall and a base. The immobilization member is arranged within the reservoir and comprises a surface that is patterned in such a way that it comprises at least one predefined immobilization area. The predefined immobilization area within the patterned surface is of a higher surface energy than the medium. The liquid droplet is disposed onto the predefined immobilization area, whereby the liquid droplet is immobilized thereon via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions.
摘要翻译：本发明提供了一种在液滴中处理生物和/或化学样品的装置和方法。该装置包括由储存器和固定构件限定的处理室。处理室还适于容纳与液滴不混溶的介质和比液滴的液体低的表面能。储存器由圆周壁和底座限定。固定构件布置在储存器内并且包括以使得其包括至少一个预定义的固定区域的方式图案化的表面。图案化表面内的预定义的固定区域具有比介质更高的表面能。将液滴设置在预定义的固定区域上，由此通过亲水亲水或疏水 - 疏水相互作用将液滴固定在其上。

3. 发明授权

US08261598B2 Apparatus for performing a reaction in a droplet and method of using the same 有权
标题翻译：用于在液滴中进行反应的装置及其使用方法
公开(公告)号：US08261598B2
公开(公告)日：2012-09-11
申请号：US12282162
申请日：2006-03-09
申请人： Nam Yong Kim , Jackie Yi Ru Ying , Yong Yeow Lee , Kwong Joo Leck
发明人： Nam Yong Kim , Jackie Yi Ru Ying , Yong Yeow Lee , Kwong Joo Leck
IPC分类号： G01N1/00
CPC分类号： G01N1/28 , B01L3/0268 , B01L3/5085 , B01L2200/026 , B01L2200/0673 , B01L2300/0609 , B01L2400/027 , G01N2035/00158 , G01N2035/1034 , G01N2035/1037
摘要： An apparatus for processing a biological and/or chemical sample in a liquid droplet (1) is provided. The apparatus includes a processing compartment (20), a base (21) and at least one circumferential wall (25). The processing compartment (20) is defined by at least a part of the base (21), at least a part of the circumferential wall (25) and an inlet member (4). The inlet member (4) is located on top of the processing compartment (20), and includes at least one droplet inlet channel (3), which extends through the inlet member (4) and includes a contraction (2) between the inlet opening (28) of the droplet inlet channel (3) to the environment and the outlet opening (27) to the processing compartment (20). There is furthermore provided a method of processing a biological and/or chemical sample in a liquid droplet (1). The method includes disposing a medium into the processing compartment (20) of the apparatus of the invention that is immiscible with the liquid droplet (1), so that the contraction (2) is immersed in the medium. The droplet (1) is disposed into the droplet inlet channel (3) such that it is located below the contraction (2) of the droplet inlet channel (3). A process is performed on the biological and/or chemical sample in the droplet (1).
摘要翻译：提供了一种用于处理液滴（1）中的生物和/或化学样品的装置。该设备包括处理室（20），基座（21）和至少一个周向壁（25）。处理室（20）由基部（21）的至少一部分，周壁（25）的至少一部分和入口部件（4）限定。入口构件（4）位于处理室（20）的顶部，并且包括至少一个液滴入口通道（3），其延伸穿过入口构件（4）并且包括在入口开口（28）和所述出口（27）连接到所述处理室（20）。此外还提供了一种在液滴（1）中处理生物和/或化学样品的方法。该方法包括将介质设置在与液滴（1）不混溶的本发明设备的处理室（20）中，使得收缩（2）浸入介质中。液滴（1）被设置在液滴入口通道（3）中，使得其位于液滴入口通道（3）的收缩（2）的下方。对液滴（1）中的生物和/或化学样品进行处理。

4. 发明申请

US20100000304A1 Apparatus For Performing a Reaction In a Droplet and Method of Using the Same 有权
标题翻译：用于在液滴中进行反应的装置及其使用方法
公开(公告)号：US20100000304A1
公开(公告)日：2010-01-07
申请号：US12282162
申请日：2006-03-09
申请人： Nam Yong Kim , Y. Jackie Ying , Yong Yeow Lee , Kwong Joo Leck
发明人： Nam Yong Kim , Y. Jackie Ying , Yong Yeow Lee , Kwong Joo Leck
IPC分类号： G01N1/00
CPC分类号： G01N1/28 , B01L3/0268 , B01L3/5085 , B01L2200/026 , B01L2200/0673 , B01L2300/0609 , B01L2400/027 , G01N2035/00158 , G01N2035/1034 , G01N2035/1037
摘要： An apparatus for processing a biological and/or chemical sample in a liquid droplet (1) is provided. The apparatus includes a processing compartment (20), a base (21) and at least one circumferential wall (25). The processing compartment (20) is defined by at least a part of the base (21), at least a part of the circumferential wall (25) and an inlet member (4). The inlet member (4) is located on top of the processing compartment (20), and includes at least one droplet inlet channel (3), which extends through the inlet member (4) and includes a contraction (2) between the inlet opening (28) of the droplet inlet channel (3) to the environment and the outlet opening (27) to the processing compartment (20). There is furthermore provided a method of processing a biological and/or chemical sample in a liquid droplet (1). The method includes disposing a medium into the processing compartment (20) of the apparatus of the invention that is immiscible with the liquid droplet (1), so that the contraction (2) is immersed in the medium. The droplet (1) is disposed into the droplet inlet channel (3) such that it is located below the contraction (2) of the droplet inlet channel (3). A process is performed on the biological and/or chemical sample in the droplet (1).
摘要翻译：提供了一种用于处理液滴（1）中的生物和/或化学样品的装置。该设备包括处理室（20），基座（21）和至少一个周向壁（25）。处理室（20）由基部（21）的至少一部分，周壁（25）的至少一部分和入口部件（4）限定。入口构件（4）位于处理室（20）的顶部，并且包括至少一个液滴入口通道（3），其延伸穿过入口构件（4）并且包括在入口开口（28）和所述出口（27）连接到所述处理室（20）。此外还提供了一种在液滴（1）中处理生物和/或化学样品的方法。该方法包括将介质设置在与液滴（1）不混溶的本发明设备的处理室（20）中，使得收缩（2）浸入介质中。液滴（1）被设置在液滴入口通道（3）中，使得其位于液滴入口通道（3）的收缩（2）的下方。对液滴（1）中的生物和/或化学样品进行处理。

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