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1. 发明授权

US06943004B1 Transformed microorganism and process for producing D-aminoacylase 失效
标题翻译：转化微生物和生产D-氨基酰化酶的方法
公开(公告)号：US06943004B1
公开(公告)日：2005-09-13
申请号：US10009782
申请日：2000-06-15
申请人： Ken-ichi Takeuchi , Yoshinao Koide , Yoshihiko Hirose , Mitsuaki Moriguchi , Kimiyasu Isobe
发明人： Ken-ichi Takeuchi , Yoshinao Koide , Yoshihiko Hirose , Mitsuaki Moriguchi , Kimiyasu Isobe
IPC分类号： C12N15/09 , C12N1/21 , C12N9/80 , C12N9/78 , C07H21/02 , C12N1/20 , C12N15/00 , C12P21/06
CPC分类号： C12N9/80
摘要： A transformed microorganism prepared by inserting into a host microorganism with zinc tolerance a D-aminoacylase-producing gene which selectively produces D-aminoacylase alone between D-aminoacylase and L-aminoacylase. A process comprising culturing the transformed microorganism in a culture medium containing zinc ion and obtaining D-aminoacylase from the culture at a high efficiency.
摘要翻译：通过将具有耐受性的D-氨基酸酰化酶产生基因插入具有耐受性的宿主微生物中的转化的微生物，其在D-氨基酸酰化酶和L-氨基酸酰化酶之间单独选择性地产生D-氨基酸酰化酶。一种方法，包括在含有锌离子的培养基中培养转化的微生物，并从培养物中以高效率获得D-氨基酰化酶。

2. 发明申请

US20100221810A1 NOVEL DIGLYCOSIDASE AND GENE ENCODING THE SAME 有权
标题翻译：新型大分子酶和基因编码它们
公开(公告)号：US20100221810A1
公开(公告)日：2010-09-02
申请号：US11997005
申请日：2006-07-27
申请人： Kazutaka Tsuruhami , Shigeharu Mori , Yoshinao Koide
发明人： Kazutaka Tsuruhami , Shigeharu Mori , Yoshinao Koide
IPC分类号： C12N9/24 , C07K14/385 , C12N15/31 , C12N15/63 , C12N1/15
CPC分类号： C12R1/80 , C12N9/2402 , C12P19/12
摘要： A novel diglycosidase produced by a microorganism belonging to the genus Penicillium, having the following physicochemical properties:(1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof; (2) optimum pH: around 4.5; (3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower; (4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5); (5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes; (6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and (7) isoelectric point (pI): about 4.3.
摘要翻译：一种由属于青霉属的微生物产生的新型二糖苷酶，具有以下物理化学性质：（1）作用和底物特异性：其作用于二糖苷，释放二糖和其糖苷配基; （2）最适pH：约4.5; （3）pH稳定性：在37℃的加工条件下在pH4.0〜8.0下稳定30分钟，即使在pH4.0以下处理后仍保持80％以上的活性; （4）最适温度：约60℃在乙酸钠 - 乙酸缓冲溶液（pH5.5）中; （5）热稳定性：在乙酸钠 - 乙酸缓冲溶液（pH5.5）中在50℃以下稳定，即使在60℃下处理40分钟后也保持45％的活性; （6）基于SDS-PAGE测定的分子量：40,000±5,000Da; 和（7）等电点（pI）：约4.3。

3. 发明授权

US07998721B2 Diglycosidase and gene encoding the same 有权
标题翻译：二糖苷酶和编码基因的基因
公开(公告)号：US07998721B2
公开(公告)日：2011-08-16
申请号：US11997005
申请日：2006-07-27
申请人： Kazutaka Tsuruhami , Shigeharu Mori , Yoshinao Koide
发明人： Kazutaka Tsuruhami , Shigeharu Mori , Yoshinao Koide
IPC分类号： C12N9/24 , C12N1/00 , C12N15/00 , C07H21/04
CPC分类号： C12R1/80 , C12N9/2402 , C12P19/12
摘要： A novel diglycosidase produced by a microorganism belonging to the genus Penicillium, having the following physicochemical properties:(1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof;(2) optimum pH: around 4.5;(3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower;(4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5);(5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes;(6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and(7) isoelectric point (pI): about 4.3.
摘要翻译：一种由属于青霉属的微生物产生的新型二糖苷酶，具有以下物理化学性质：（1）作用和底物特异性：其作用于二糖苷，释放二糖和其糖苷配基; （2）最适pH：约4.5; （3）pH稳定性：在37℃的加工条件下在pH4.0〜8.0下稳定30分钟，即使在pH4.0以下处理后仍保持80％以上的活性; （4）最适温度：约60℃在乙酸钠 - 乙酸缓冲溶液（pH5.5）中; （5）热稳定性：在乙酸钠 - 乙酸缓冲溶液（pH5.5）中在50℃以下稳定，即使在60℃下处理40分钟后也保持45％的活性; （6）基于SDS-PAGE测定的分子量：40,000±5,000Da; 和（7）等电点（pI）：约4.3。

4. 发明授权

US06303357B1 L-&agr;-glycerophosphate oxidase gene, recombinant DNA, and method for producing modified L-&agr;-glycerophosphate oxidase gene 有权
标题翻译： L-α-甘油磷酸酯氧化酶基因，重组DNA，以及生产修饰的L-α-甘油磷酸氧化酶基因的方法
公开(公告)号：US06303357B1
公开(公告)日：2001-10-16
申请号：US09537682
申请日：2000-03-29
申请人： Kenichi Takeuchi , Yoshinao Koide , Yuji Nakanishi , Satoru Suzuki
发明人： Kenichi Takeuchi , Yoshinao Koide , Yuji Nakanishi , Satoru Suzuki
IPC分类号： C12N904
CPC分类号： C12N9/0006
摘要： This invention provides an L-&agr;-glycerophosphate oxidase (GPO) having excellent properties such as stability, heat resistance and reactivity. A recombinant GPO obtained by replacing an amino acid of a specified position of an amino acid sequence deduced from the Enterococcus faecium No. 7044 GPO gene or DNA coding for the GPO with other amino acid has excellent thermal stability and reactivity. That is, the invention provides modified forms of the GPO having the amino acid sequence of Sequence No. 1 in the Sequence Listing, in which the 130-position leucine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 225-position serine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 298-position threonine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 420-position aspartic acid counting from the N-terminus of the GPO is replaced by other amino acid.
摘要翻译：本发明提供了具有优异性能如稳定性，耐热性和反应性的L-α-甘油磷酸酯氧化酶（GPO）。通过将来自屎肠球菌屎肠杆菌No.7044GPO基因的氨基酸序列的特定位置的氨基酸或其它氨基酸的编码GPO的DNA取代而获得的重组GPO具有优异的热稳定性和反应性。也就是说，本发明提供具有序列表中序列号1的氨基酸序列的GPO的修饰形式，其中从GPO的N末端计数的130位亮氨酸被其它氨基酸和/ 或从GPO的N末端计数的225位丝氨酸被其他氨基酸替代，和/或从GPO的N末端计数的298位苏氨酸被其它氨基酸和/或420- 从GPO的N末端计数天冬氨酸的位置被其他氨基酸取代。

5. 发明授权

US5550041A Caffeine demethylate gene-containing DNA fragment and microbial process for producing 3-methyl-7-alkylxanthine 失效
标题翻译：含咖啡因去甲基化物基因的DNA片段和生产3-甲基-7-烷基黄嘌呤的微生物过程
公开(公告)号：US5550041A
公开(公告)日：1996-08-27
申请号：US324483
申请日：1994-10-18
申请人： Yoshinao Koide , Seiji Nakane , Yutaka Imai
发明人： Yoshinao Koide , Seiji Nakane , Yutaka Imai
IPC分类号： C07D473/06 , C07D473/08 , C07D473/12 , C12N1/21 , C12N9/10 , C12N15/60 , C12P17/18 , C12N9/14 , C12N9/78
CPC分类号： C12P17/182 , C07D473/06 , C07D473/08 , C07D473/12 , C12N9/1007
摘要： A DNA fragment containing a caffeine demethylase gene produced by a microorganism belonging to the genus Pseudomonas and capable of assimilating caffeine and a process for producing a 3-methyl-7-alkylxanthine comprising cultivating a novel bacterium strain of the genus Pseudomonas having been transformed with a recombinant DNA having integrated therein the above-mentioned DNA fragment in a nutrient culture medium containing a 1,3-dimethyl-7-alkylxanthine to produce a 3-methyl-7-alkylxanthine in the culture and recovering the produced 3-methyl-7-alkylxanthine from the culture are disclosed, as well as a process for producing 3-methyl-7-propylxanthine, comprising cultivating a microorganism capable of converting 1,3-dimethyl-7-propylxanthine to 3-methyl-7-propylxanthine or a mutant thereof in a nutrient culture medium containing 1,3-dimethyl-7-propylxanthine, to produce 3-methyl-7-propylxanthine in the culture and recovering the produced 3-methyl-7-propylxanthine from the culture.
摘要翻译：一种含有由属于假单胞菌属微生物并能够同化咖啡因的微生物产生的咖啡因去甲基化酶基因的DNA片段和一种3-甲基-7-烷基黄嘌呤的制备方法，包括培养已被转化了的假单胞菌属的新型细菌菌株在含有1,3-二甲基-7-烷基黄嘌呤的营养培养基中将上述DNA片段整合到培养物中以产生3-甲基-7-烷基黄嘌呤的重组DNA，并回收生成的3-甲基-7- 公开了来自培养物的烷基黄嘌呤，以及生产3-甲基-7-丙基黄嘌呤的方法，包括培养能够将1,3-二甲基-7-丙基黄嘌呤转化为3-甲基-7-丙基黄嘌呤的微生物或其突变体在含有1,3-二甲基-7-丙基黄嘌呤的营养培养基中，在培养物中产生3-甲基-7-丙基黄嘌呤，并从培养物中回收生成的3-甲基-7-丙基黄嘌呤。

6. 发明授权

US5290694A Recombinant DNA, bacterium of the genus Pseudomonas containing it, and process for preparing lipase by using it 失效
标题翻译：重组DNA，含有假单胞菌属的细菌，以及通过使用它制备脂肪酶的方法
公开(公告)号：US5290694A
公开(公告)日：1994-03-01
申请号：US317827
申请日：1989-02-28
申请人： Yuji Nakanishi , Yoshiaki Kurono , Yoshinao Koide , Teruhiko Beppu
发明人： Yuji Nakanishi , Yoshiaki Kurono , Yoshinao Koide , Teruhiko Beppu
IPC分类号： C12N1/21 , C12N9/20 , C12N15/09 , C12R1/38 , C12R1/40 , C12N15/31 , C12N15/55 , C12N15/67 , C12N15/78
CPC分类号： C12N9/20
摘要： A recombinant DNA capable of being replicated in a bacterium of the genus Pseudomonas is disclosed. The DNA contains a wide host range plasmid vector having a gene that codes for lipase. A process for producing lipase by transforming a host bacterium with the recombinant DNA is also described.
摘要翻译：公开了能够在假单胞菌属细菌中复制的重组DNA。 DNA含有具有编码脂肪酶基因的宽宿主质粒载体。还描述了通过用重组DNA转化宿主细菌来生产脂肪酶的方法。

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