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1. 发明授权

US06472182B1 Process for producing transglutaminase by microorganism 失效
标题翻译：通过微生物生产转谷氨酰胺酶的方法
公开(公告)号：US06472182B1
公开(公告)日：2002-10-29
申请号：US08952959
申请日：1997-12-04
申请人： Katsunori Kobayashi , Shigeru Yamanaka , Yuko Tanita , Naoko Tsuyoshi , Ryosuke Fudo , Junko Shinozaki , Kenzo Yokozeki , Shunichi Suzuki
发明人： Katsunori Kobayashi , Shigeru Yamanaka , Yuko Tanita , Naoko Tsuyoshi , Ryosuke Fudo , Junko Shinozaki , Kenzo Yokozeki , Shunichi Suzuki
IPC分类号： C12P2104
CPC分类号： C12N9/1044 , C12Q1/16 , C12Y203/02013
摘要： The present invention provides a process for producing a transglutaminase, which comprises culturing microorganisms of any of the genus Micrococcus, Clostridium, Torulopsis, Rhizopus and Monascus in a medium to produce the intended transglutaminase in the medium or in the cells of the microorganisms and then isolating the transglutaminase, and a process for producing a gelled proten with the thus-obtained transglutaminase. According to the process of the present invention, transglutaminase can be rapidly produced at a low cost.
摘要翻译：本发明提供一种转谷氨酰胺酶的制造方法，其包括在培养基中培养微球菌属，梭菌属，梭菌属，根霉属和红曲霉属中的任一种的微生物，以在培养基中或微生物细胞中产生预期的转谷氨酰胺酶，然后分离转谷氨酰胺酶，以及由此获得的转谷氨酰胺酶制备凝胶化的保护膜的方法。根据本发明的方法，可以低成本快速生产转谷氨酰胺酶。

2. 发明授权

US5731183A Bacillus-derived transglutaminase 失效
标题翻译：芽孢杆菌衍生的转谷氨酰胺酶
公开(公告)号：US5731183A
公开(公告)日：1998-03-24
申请号：US596864
申请日：1996-02-09
申请人： Katsunori Kobayashi , Shigeru Yamanaka , Kiyoshi Miwa , Shunichi Suzuki , Yuzuru Eto , Yuko Tanita , Kenzo Yokozeki , Kenichi Hashiguchi
发明人： Katsunori Kobayashi , Shigeru Yamanaka , Kiyoshi Miwa , Shunichi Suzuki , Yuzuru Eto , Yuko Tanita , Kenzo Yokozeki , Kenichi Hashiguchi
IPC分类号： C12N15/09 , A23J3/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/54 , C12P21/04 , C12R1/125 , C12R1/19
CPC分类号： C12N9/1044 , Y10S435/839
摘要： The present invention relates to (1) a transglutaminase (hereinafter referred to as TG) isolated from a Bacilli such as those of Bacillus subtilis, (2) a fraction having transglutaminase activity, and (3) a method for producing a protein, a non-proteinaceous amino acid polymer, a peptide or derivatives thereof having a crosslinked structure, by crosslinking the glutamine and lysine residues in the same with the TG or the fraction having TG activity to thereby form intermolecular or intramolecular, crosslinked .epsilon.-(.gamma.-Glu)-Lys bonds. The present invention also relates to (4) a DNA coding for a TG derived from a Bacilli such as Bacillus subtilis, (5) a vector comprising said DNA coding for the TG, (6) a cell transformed with the vector, and (7) a method for producing a Bacillus-derived transglutaminase by incubating the transformant. The crosslinked polymers produced using the Bacillus-derived TG of the present invention can be used in foods such as tofu (soybean curd), pudding, yogurt, cheese, ground fish meat, boiled fish paste, sausage and other fish and meat products and also in cosmetics, etc.
摘要翻译：本发明涉及（1）从枯草芽孢杆菌等芽孢分离的转谷氨酰胺酶（以下称为TG），（2）具有转谷氨酰胺酶活性的级分，（3）蛋白质的制造方法，蛋白质氨基酸聚合物，具有交联结构的肽或其衍生物，通过与TG或具有TG活性的级分交联谷氨酰胺和赖氨酸残基，从而形成分子间或分子内交联的ε-（γ-Glu） -Lys债券。本发明还涉及（4）编码来自芽孢杆菌如枯草芽孢杆菌的TG的DNA，（5）包含编码TG的所述DNA的载体，（6）用载体转化的细胞和（7 ）通过温育转化体来生产芽孢杆菌来源的转谷氨酰胺酶的方法。使用本发明的芽孢杆菌来源的TG制备的交联聚合物可以用于诸如豆腐（豆腐），布丁，酸奶，奶酪，地面鱼肉，煮鱼酱，香肠和其他鱼和肉制品的食品中化妆品等

3. 发明授权

US5948662A Bacillus-derived transglutaminase 失效
标题翻译：芽孢杆菌衍生的转谷氨酰胺酶
公开(公告)号：US5948662A
公开(公告)日：1999-09-07
申请号：US989667
申请日：1997-12-12
申请人： Katsunori Kobayashi , Shigeru Yamanaka , Kiyoshi Miwa , Shunichi Suzuki , Yuzuru Eto , Yuko Tanita , Kenzo Yokozeki , Kenichi Hashiguchi
发明人： Katsunori Kobayashi , Shigeru Yamanaka , Kiyoshi Miwa , Shunichi Suzuki , Yuzuru Eto , Yuko Tanita , Kenzo Yokozeki , Kenichi Hashiguchi
IPC分类号： C12N15/09 , A23J3/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/54 , C12P21/04 , C12R1/125 , C12R1/19
CPC分类号： C12N9/1044 , Y10S435/839
摘要： A method for processing a protein, a non-proteinaceous amino acid polymer, or a non-proteinaceous amino acid polymer, or a peptide or derivatives thereof having a crosslinked structure, which entails contacting glutamine and lysine residues in a protein, a non-proteinaceous amino acid polymer, a peptide or derivatives thereof with a transglutaminase obtained from Bacillus subtilus to form intermolecular or intramolecular, crosslinked .epsilon.(.delta.-Glu)-Lys bonds between or in the molecules of the protein, non-proteinaceous amino acid polymer, peptide or derivatives thereof, wherein the transglutaminase has the physicochemical properties described herein.
摘要翻译：用于处理蛋白质，非蛋白质氨基酸聚合物或非蛋白质氨基酸聚合物的方法或具有交联结构的肽或其衍生物，其需要使蛋白质中的谷氨酰胺和赖氨酸残基接触，非蛋白质氨基酸聚合物，肽或其衍生物与从枯草芽孢杆菌获得的转谷氨酰胺酶在蛋白质，非蛋白质氨基酸聚合物，肽或肽的分子之间或之内形成分子间或分子内交联的ε（δ-Glu）-Lys键其衍生物，其中转谷氨酰胺酶具有本文所述的物理化学性质。

4. 发明授权

US08394940B2 Oxidase gene and method for producing 3-indole-pyruvic acid by utilizing the gene 有权
标题翻译：氧化酶基因及利用该基因产生3-吲哚 - 丙酮酸的方法
公开(公告)号：US08394940B2
公开(公告)日：2013-03-12
申请号：US12711719
申请日：2010-02-24
申请人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C07H21/04
CPC分类号： C12P17/10 , C12N9/0022
摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.
摘要翻译：本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。

5. 发明申请

US20100317068A1 METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME 有权
标题翻译：生产用于其的丝氨酸衍生物和蛋白质的方法
公开(公告)号：US20100317068A1
公开(公告)日：2010-12-16
申请号：US12836984
申请日：2010-07-15
申请人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
发明人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
IPC分类号： C12P13/06 , C07H21/00 , C12N9/10 , C12N1/21
CPC分类号： C12N9/1029 , C12P13/04
摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.
摘要翻译：本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质（B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物。

6. 发明授权

US07320794B2 Transporter protein 失效
标题翻译：转运蛋白
公开(公告)号：US07320794B2
公开(公告)日：2008-01-22
申请号：US11276614
申请日：2006-03-08
申请人： Shunichi Suzuki , Kenzo Yokozeki , Peter Henderson
发明人： Shunichi Suzuki , Kenzo Yokozeki , Peter Henderson
IPC分类号： A61K39/00 , A61K39/38 , C12P1/00 , C12P21/06 , C07K1/00 , C07H21/04
CPC分类号： C07K14/195
摘要： A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to uptake hydantoin compounds is obtained by introducing and expressing the novel gene, called mhp, using gene recombination techniques.
摘要翻译：描述了具有转运乙内酰脲化合物的活性的新型蛋白质，以及表达该转运蛋白的重组体。从微型液化酵母菌株AJ3912开始，发现了一种能够转运乙内酰脲化合物的蛋白质的新基因。通过使用基因重组技术引入和表达新的基因，称为mhp，获得具有优异吸收乙内酰脲化合物能力的重组体。

7. 发明申请

US20060183190A1 NOVEL TRANSPORTER PROTEIN 失效
标题翻译：新型运输蛋白
公开(公告)号：US20060183190A1
公开(公告)日：2006-08-17
申请号：US11276614
申请日：2006-03-08
申请人： Shunichi Suzuki , Kenzo Yokozeki , Peter Henderson
发明人： Shunichi Suzuki , Kenzo Yokozeki , Peter Henderson
IPC分类号： C07K14/195 , C07H21/04 , C12P21/06 , C12N15/74
CPC分类号： C07K14/195
摘要： A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to uptake hydantoin compounds is obtained by introducing and expressing the novel gene, called mhp, using gene recombination techniques.
摘要翻译：描述了具有转运乙内酰脲化合物的活性的新型蛋白质，以及表达该转运蛋白的重组体。从微型液化酵母菌株AJ3912开始，发现了一种能够转运乙内酰脲化合物的蛋白质的新基因。通过使用基因重组技术引入和表达新的基因，称为mhp，获得具有优异吸收乙内酰脲化合物能力的重组体。

8. 发明授权

US06303353B1 Process for preparing xylitol 失效
标题翻译：制备木糖醇的方法
公开(公告)号：US06303353B1
公开(公告)日：2001-10-16
申请号：US09509547
申请日：2000-04-12
申请人： Masakazu Sugiyama , Shunichi Suzuki , Yasuhiro Mihara , Kenichi Hashiguchi , Kenzo Yokozeki
发明人： Masakazu Sugiyama , Shunichi Suzuki , Yasuhiro Mihara , Kenichi Hashiguchi , Kenzo Yokozeki
IPC分类号： C12P718
CPC分类号： C12P7/18
摘要： Xylitol is produced by contacting D-arabitol with a microorganism that belongs to the genus Gluconobacter, Acetobacter, Achromobacter, Agrobacterium, Arthrobacter, Azotobacter, Brevibacterium, Corynebacterium, Erwinia, Flavobacterium, Micrococcus, Nocardia, Planococcus, Pseudomonas, Rhodococcus, Morganella, Actinomadura, Actinomyces, or Streptomyces and is capable of converting D-arabitol to xylitol, and recovering xylitol thus produced.
摘要翻译：通过使D-阿拉伯糖醇与属于葡萄糖酸杆菌属，醋杆菌属，无色杆菌属，农杆菌属，节杆菌属，固氮菌属，短杆菌属，棒状杆菌属，欧文氏菌属，黄杆菌属，微球菌属，诺卡氏菌属，普兰克菌属，假单胞菌属，红球菌属，摩根氏菌属，放线菌属，放线菌或链霉菌，能够将D-阿拉伯糖醇转化为木糖醇，并回收如此生产的木糖醇。

9. 发明授权

US08518665B2 Methods for making 3-indole-pyruvic acid from tryptophan using a tryptophan deaminase 有权
标题翻译：使用色氨酸脱氨酶从色氨酸制备3-吲哚 - 丙酮酸的方法
公开(公告)号：US08518665B2
公开(公告)日：2013-08-27
申请号：US13684888
申请日：2012-11-26
申请人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C12P1/00 , C12P21/06
CPC分类号： C12P17/10 , C12N9/0022
摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.
摘要翻译：本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。

10. 发明授权

US08372607B2 Method for producing serine derivative and protein used for the same 有权
标题翻译：用于制备丝氨酸衍生物和蛋白质的方法
公开(公告)号：US08372607B2
公开(公告)日：2013-02-12
申请号：US12836984
申请日：2010-07-15
申请人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
发明人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
IPC分类号： C12P13/06 , C07H21/00 , C12N1/21
CPC分类号： C12N9/1029 , C12P13/04
摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.
摘要翻译：本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质（B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物。

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