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    • 5. 发明申请
    • INHIBITION OF ICE CRYSTAL GROWTH
    • 冰晶生长的抑制
    • US20100151096A1
    • 2010-06-17
    • US12624527
    • 2009-11-24
    • Srinivasan Damodaran
    • Srinivasan Damodaran
    • A23L1/48C07K7/06C07K7/08C07H21/04C12P21/06
    • C12P21/06A23G9/38A23J3/342A23L3/37A23V2002/00C07K7/06C07K14/78A23V2200/206A23V2250/54
    • Antifreeze polypeptides, antifreeze compositions including the polypeptides, nucleotides encoding the antifreeze polypeptides, methods of making antifreeze compositions, and methods of inhibiting ice crystal growth are provided herein. The peptides are based on the primary sequence of collagen and include those having a molecular weight between about 500-7000 Da. The peptides preferably include cationic polypeptides. The methods of making antifreeze compositions include digesting collagen or gelatin into hydrolysates with peptides having molecular weights between about 500-7000 Da. The digestions are performed with proteases and/or non-enzymatic hydrolysis. The methods of inhibiting ice crystal growth include adding the antifreeze polypeptides or compositions described herein to a composition to be frozen. The methods may be used to inhibit ice crystal growth in frozen food products.
    • 本文提供了抗冻多肽,包含多肽的防冻组合物,编码抗冻多肽的核苷酸,制备防冻组合物的方法和抑制冰晶生长的方法。 肽基于胶原的一级序列,包括分子量在约500-7000Da之间的肽。 肽优选包括阳离子多肽。 制备防冻组合物的方法包括用分子量在约500-7000Da之间的肽将胶原蛋白或明胶消化成水解产物。 消化用蛋白酶和/或非酶水解进行。 抑制冰晶生长的方法包括将本文所述的防冻多肽或组合物加入待冷冻的组合物中。 该方法可用于抑制冷冻食品中的冰晶生长。