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    • 5. 发明授权
    • Detection of salmonella enteritidis and other pathogenic microorganisms
and monoclonal antibody useful therefor
    • 检测肠炎沙门氏菌和其他病原微生物和有用的单克隆抗体
    • US5807694A
    • 1998-09-15
    • US524506
    • 1995-09-07
    • Jerzy Zawistowski
    • Jerzy Zawistowski
    • G01N33/569C12N1/20G01N33/53
    • G01N33/56916G01N2333/255
    • A method of testing for the presence of a pathogenic microorganism, notably Salmonella enteritidis, in a foodstuff, e.g. the contents of an egg, or other materials. In a particularly preferred form for testing eggs, the method involves homogenizing a sample of the egg, incubating the egg contents to enrich any pathogen present, adding a detergent to release antigens from pathogen cell surfaces, heating the egg contents to gell them and then carrying out a dot-blot assay of the gelled egg material to detect the presence of the antigens. The method is particularly applicable to D.sub.1 serovars of Salmonella enteritidis. The antibody used in the method is preferably a novel IgG.sub.2.alpha. type immunoglobulin having a lambda light chain, designated as MAb 2F11, which can also be used for other methods of testing for Salmonella enteritidis and other D.sub.1 serovars, and for passive protection of poultry against infections by such pathogens.
    • 一种在食品中测定病原微生物,特别是肠炎沙门氏菌的存在的方法,例如, 蛋的内容或其他材料。 在用于测试蛋的特别优选的形式中,该方法包括使蛋的样品均质化,孵育卵内容物以富集存在的任何病原体,加入洗涤剂以从病原体细胞表面释放抗原,加热蛋内含物凝胶,然后携带 进行凝胶蛋材料的斑点印迹测定以检测抗原的存在。 该方法特别适用于肠炎沙门氏菌的D1型血清型。 该方法中使用的抗体优选是具有λ轻链的新型IgG2α型免疫球蛋白,其命名为MAb 2F11,其也可用于其它测试肠炎沙门氏菌和其他D1血清型的测试方法,并用于家禽的被动保护 这种病原体的感染。