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1. 发明授权

US08828721B1 Method of myelinating isolated motoneurons 有权
标题翻译：孤立运动神经元的髓鞘化方法
公开(公告)号：US08828721B1
公开(公告)日：2014-09-09
申请号：US12788732
申请日：2010-05-27
申请人： James Hickman , John Rumsey
发明人： James Hickman , John Rumsey
IPC分类号： C12N5/0793 , C12N5/079
CPC分类号： C12N5/0619 , C12N5/0622 , C12N2502/081 , C12N2502/086 , C12N2533/30 , C12N2533/32
摘要： The present invention provides a method of inducing myelination of isolated motoneurons by preparing a non-biological substrate having thereon a covalently attached monolayer of DETA; depositing isolated motoneurons on the substrate in a defined serum-free medium; plating isolated Schwann cells cultured in the defined serum-free medium onto the motoneurons, thereby initiating a co-culture; and passaging the co-culture as necessary into fresh, defined serum-free medium supplemented with L-ascorbic acid at least until the motoneurons form Nodes of Ranvier indicative of myelination. The invention also includes a method of testing for new drugs effective in demyelinating diseases. Additionally, cellular products provided by the invention include an isolated motoneurons myelinated or remyelinated in vitro according to the methods disclosed.
摘要翻译：本发明提供了通过制备其上具有共价连接的DETA单层的非生物底物来诱导分离的运动神经元的髓鞘形成的方法; 在限定的无血清培养基中在基底上沉积分离的运动神经元; 将在定义的无血清培养基中培养的分离的雪旺氏细胞电镀到运动神经元上，从而开始共培养; 并根据需要将共培养物传代至补充有L-抗坏血酸的新鲜定义的无血清培养基中，至少直到运动神经元形成指示髓鞘形成的Ranvier的节点。本发明还包括测试有效脱髓鞘疾病的新药的方法。此外，本发明提供的细胞产品包括根据所公开的方法在体外有髓鞘或髓鞘内分离的运动神经元。

2. 发明授权

US4692017A Toner transfer apparatus 失效
标题翻译：调色剂转印装置
公开(公告)号：US4692017A
公开(公告)日：1987-09-08
申请号：US812027
申请日：1985-12-23
申请人： Andrzej Maczuszenko , Sotos M. Theodoulou , John Rumsey
发明人： Andrzej Maczuszenko , Sotos M. Theodoulou , John Rumsey
IPC分类号： G03G15/08 , G03G15/09
CPC分类号： G03G15/09 , G03G15/0898
摘要： A toner system for delivering powder toner from a storage hopper to an element carrying an electrostatic pattern of charge to be toned before transfer to a receptor such as paper includes a scavenger which, when coupled to the toner system can be actuated to develop a slight negative pressure in the toner system sufficient to entrap flow enhancers such as zinc stearate and to remove the non-magnetic zinc stearate from the toner system. The scavenger includes baffles for collecting particles and means for developing the negative pressure in the toner system.The toner system also includes a pair of magnetic clips for positioning on the metering blade of the toner system to define the width of the toner path being transfered from the toner system to the electrostatic image on the dielectric surface and a cleaning device which periodically moves along the metering blade to clean the front of the metering blade of any accumulated toner.
摘要翻译：用于将粉末调色剂从存储料斗输送到在转移到诸如纸之类的受体之前要调色的静电荷模式的元件的调色剂系统包括清除剂，当与所述调色剂系统耦合时，所述清除剂可被致动以产生轻微的负该调色剂体系中的压力足以引起流动增强剂如硬脂酸锌，并从调色剂体系中除去非磁性的硬脂酸锌。清除剂包括用于收集颗粒的挡板和用于显影调色剂系统中负压的装置。调色剂系统还包括一对磁性夹子，用于定位在调色剂系统的计量刀片上，以限定调色剂路径从调色剂系统转移到电介质表面上的静电图像的宽度，以及周期性地沿着计量刀片清洁任何累积调色剂的计量刀片的前部。

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