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    • 10. 发明申请
    • FLAVIN ENZYME HAVING FLAVONOL 8-HYDROXYLASE ACTIVITY AND USE THEREOF
    • 具有维生素8羟基酶活性的FLAVIN ENZYME及其用途
    • US20130145496A1
    • 2013-06-06
    • US13816813
    • 2011-08-18
    • Hideyuki Suzuki
    • Hideyuki Suzuki
    • C12N15/82C12N9/00
    • C12N15/8261C12N9/0071C12N15/825C12P17/06
    • The purpose of the present invention is to provide a novel flavonol 8-hydroxylase. The present invention relates to a flavin enzyme protein having a flavonol 8-hydroxylase activity, and a polynucleotide etc. encoding the same, and so on. The present invention provides: a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1 or 3; a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID No. 2; an expression vector and transformant comprising the polynucleotide; a method for screening a plant which blooms one or more yellow coloured flowers by using the polynucleotide; a method for producing a plant which blooms one or more yellow coloured flowers by introducing the polynucleotide into host cells; and a method for producing a flavin enzyme protein having a flavonol 8-hydroxylase activity, using the transformant.
    • 本发明的目的是提供一种新的黄酮醇8-羟化酶。 本发明涉及具有黄酮醇8-羟化酶活性的黄素酶蛋白质及其编码的多核苷酸等。 本发明提供:包含SEQ ID NO:1或3的核苷酸序列的多核苷酸; 编码由SEQ ID No.2的氨基酸序列组成的蛋白质的多核苷酸; 包含所述多核苷酸的表达载体和转化体; 用于筛选通过使用所述多核苷酸使一种或多种黄色花朵绽放的植物的方法; 一种通过将多核苷酸引入宿主细胞来生产一种或多种黄色花朵的植物的方法; 以及使用该转化体生产黄酮醇8-羟化酶活性的黄素酶蛋白质的方法。