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    • 8. 发明授权
    • Escherichia coli Candida maltosa Saccharomyces cerevisiae shuttle
vectors and method for making
    • 大肠杆菌麦芽糖酵母(Saccharomyces cerevisiae)梭菌载体及其制备方法
    • US4879230A
    • 1989-11-07
    • US813193
    • 1985-12-24
    • Masamichi TakagiKeiji YanoIchiro ShibuyaMinoru Morikawa
    • Masamichi TakagiKeiji YanoIchiro ShibuyaMinoru Morikawa
    • C12N15/81
    • C12N15/81
    • The present invention relates to plasmids whose hosts can be Escherichia coli and some kinds of yeasts, namely, shuttle vectors, as well as to processes for producing said plasmids. There are provided in the present invention (1) plasmids containing an autonomously replicating sequence of Candida maltosa, Leu 2 gene derived from Saccharomyces cerevisiae and an ampicillin resistance gene and (2) plasmids further containing a tetracycline resistance gene as well as the genes described in (1).The plasmids (shuttle vectors) of the present invention can be utilized as follows. A useful foreign gene is inserted into plasmids of the present invention; using the resulting new plasid, Escherichia coli is transformed and cultured in order to obtain the plasmid in a large amount; and using this plasmid, Saccharomyces cerevisiae or Candida maltosa as a host is allowed to produce useful substances such as hormones and enzymes on a large scale.
    • 本发明涉及其宿主可以是大肠杆菌和一些种类的酵母的质粒,即穿梭载体,以及生产所述质粒的方法。 在本发明中提供了(1)含有自愿复制的假丝酵母(Candida maltosa)的质粒,来源于酿酒酵母的Leu 2基因和氨苄青霉素抗性基因的质粒和(2)进一步含有四环素抗性基因的质粒以及描述于 (1)。 本发明的质粒(穿梭载体)可以如下使用。 将有用的外源基因插入本发明的质粒中; 使用所得的新的质粒,转化和培养大肠杆菌以获得大量的质粒; 使用该质粒,可以将酿酒酵母或麦芽假丝酵母作为宿主大量产生有用的物质如激素和酶。