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1. 发明授权

US07323322B2 Ethanol production from transformed Zymobacter microorganisms 失效
标题翻译：来自转化的酵母微生物的乙醇生产
公开(公告)号：US07323322B2
公开(公告)日：2008-01-29
申请号：US10901119
申请日：2004-07-29
申请人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
发明人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
IPC分类号： C12P7/06 , C12N9/26 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/065 , C12N9/2445 , C12P7/10 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： This invention provides transformed microorganisms which can produce ethanol from cellooligosaccharide, by introducing β-glucosidase gene by recombinant DNA method, into microorganisms belonging to genus Zymobacter which cannot utilize cellooligosaccharide.
摘要翻译：本发明提供了通过将β-葡糖苷酶基因通过重组DNA方法引入到不溶于低聚果糖的酵母属属的微生物中的，可以从纤维寡糖生产乙醇的转化微生物。

2. 发明申请

US20070298476A1 Pentose-fermentative transformed Zymobacter microorganisms 失效
标题翻译：戊糖发酵转化酵母菌属微生物
公开(公告)号：US20070298476A1
公开(公告)日：2007-12-27
申请号：US10901471
申请日：2004-07-29
申请人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
发明人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
IPC分类号： C12P7/06 , C12M1/00 , C12N1/20 , C12N15/00
CPC分类号： C12P7/08 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12P7/065 , C12Y202/01001 , C12Y202/01002 , C12Y207/01017 , C12Y503/01005 , Y02E50/17
摘要： This invention provides transformed microorganisms which can produce ethanol from pentose, by introducing pentose metabolizing enzymes into microorganisms belonging to genus Zymobacter which cannot utilize pentose.
摘要翻译：本发明提供了可以从戊糖产生乙醇的转化微生物，其通过将戊糖代谢酶引入不能利用戊糖的酵母属属的微生物中。

3. 发明申请

US20100311140A1 BACTERIUM CAPABLE OF FERMENTING GLUCOSE, MANNOSE AND XYLOSE SIMULTANEOUSLY, AND METHOD FOR PRODUCTION OF BIOETHANOL USING THE BACTERIUM 失效
标题翻译：可同时发酵葡萄糖，曼尼斯和XYLOSE的细菌，以及使用细菌生产生物柴油的方法
公开(公告)号：US20100311140A1
公开(公告)日：2010-12-09
申请号：US12681520
申请日：2008-10-03
申请人： Hideshi Yanase , Kenji Okamoto
发明人： Hideshi Yanase , Kenji Okamoto
IPC分类号： C12P7/10 , C12N1/21
CPC分类号： C12P7/10 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.
摘要翻译：目的是开发能够同时发酵葡萄糖，甘露糖和木糖的细菌，其可以发酵纤维素型或木质纤维素型生物质资源的糖化溶液以产生乙醇，并构建节能高效生物乙醇转化处理。因此，所公开的是运动发酵单胞菌细菌，其通过利用同源重组方法将编码来自大肠杆菌的磷酸甘露糖异构酶的基因整合到位于染色体上的左旋糖蔗糖酶基因中，然后引入重组DNA 通过将分别来自大肠杆菌的编码木糖异构酶，木酮糖激酶，转醛醇酶和转酮酶的基因的DNA片段与载体结合。还公开了通过在固定有运动发酵单胞菌细菌的系统中连续发酵纤维素型生物质资源的糖化溶液来生产乙醇的方法。

4. 发明授权

US08383377B2 Bacterium capable of fermenting glucose, mannose and xylose simultaneously, and method for production of bioethanol using the bacterium 失效
标题翻译：能够同时发酵葡萄糖，甘露糖和木糖的细菌，以及使用该细菌生产生物乙醇的方法
公开(公告)号：US08383377B2
公开(公告)日：2013-02-26
申请号：US12681520
申请日：2008-10-03
申请人： Hideshi Yanase , Kenji Okamoto
发明人： Hideshi Yanase , Kenji Okamoto
IPC分类号： C12P7/06 , C12P7/10 , C12N1/20 , C12N1/00 , C12N15/00
CPC分类号： C12P7/10 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.
摘要翻译：目的是开发能够同时发酵葡萄糖，甘露糖和木糖的细菌，其可以发酵纤维素型或木质纤维素型生物质资源的糖化溶液以产生乙醇，并构建节能高效生物乙醇转化处理。因此，所公开的是运动发酵单胞菌细菌，其通过利用同源重组方法将编码来自大肠杆菌的磷酸甘露糖异构酶的基因整合到位于染色体上的左旋糖蔗糖酶基因中，然后引入重组DNA 通过将分别来自大肠杆菌的编码木糖异构酶，木酮糖激酶，转醛醇酶和转酮酶的基因的DNA片段与载体结合。还公开了通过在固定有运动发酵单胞菌细菌的系统中连续发酵纤维素型生物质资源的糖化溶液来生产乙醇的方法。

5. 发明申请

US20090280547A1 Cellooligosaccharide-fermentative zymobacter transformed microorganisms 审中-公开
标题翻译：低聚糖发酵酵母菌转化的微生物
公开(公告)号：US20090280547A1
公开(公告)日：2009-11-12
申请号：US11979022
申请日：2007-10-30
申请人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
发明人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
IPC分类号： C12P7/10 , C12N11/00 , C12M1/00
CPC分类号： C12P7/065 , C12N9/2445 , C12P7/10 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： This invention provides transformed microorganisms which can produce ethanol from cellooligosaccharide, by introducing β-glucosidase gene by recombinant DNA method, into microorganisms belonging to genus Zymobacter which cannot utilize cellooligosaccharide.
摘要翻译：本发明提供了通过将β-葡糖苷酶基因通过重组DNA方法引入到不溶于低聚果糖的酵母属属的微生物中的，可以从纤维寡糖生产乙醇的转化微生物。

6. 发明授权

US07569379B2 Pentose-fermentative transformed zymobacter microorganisms 失效
标题翻译：戊糖发酵转化的酵母属微生物
公开(公告)号：US07569379B2
公开(公告)日：2009-08-04
申请号：US10901471
申请日：2004-07-29
申请人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
发明人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
IPC分类号： C12P7/00 , C12P7/06 , C12N1/21
CPC分类号： C12P7/08 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12P7/065 , C12Y202/01001 , C12Y202/01002 , C12Y207/01017 , C12Y503/01005 , Y02E50/17
摘要： This invention provides transformed microorganisms which can produce ethanol from pentose, by introducing pentose metabolizing enzymes into microorganisms belonging to genus Zymobacter which cannot utilize pentose.
摘要翻译：本发明提供了可以从戊糖产生乙醇的转化微生物，其通过将戊糖代谢酶引入不能利用戊糖的酵母属属的微生物中。

7. 发明申请

US20050074858A1 Cellooligosaccharide-fermentative Zymobacter transformed microorganisms 失效
标题翻译：纤维寡糖发酵酵母菌转化微生物
公开(公告)号：US20050074858A1
公开(公告)日：2005-04-07
申请号：US10901119
申请日：2004-07-29
申请人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
发明人： Hideshi Yanase , Kenji Okamoto , Takahide Takadera , Atsuko Sugiura
IPC分类号： C12N1/21 , C12N9/42 , C12P7/06 , C12P7/10
CPC分类号： C12P7/065 , C12N9/2445 , C12P7/10 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： This invention provides transformed microorganisms which can produce ethanol from cellooligosaccharide, by introducing β-glucosidase gene by recombinant DNA method, into microorganisms belonging to genus Zymobacter which cannot utilize cellooligosaccharide.
摘要翻译：本发明提供了通过将β-葡糖苷酶基因通过重组DNA方法引入到不溶于低聚果糖的酵母属属的微生物中的，可以从纤维寡糖生产乙醇的转化微生物。

8. 发明授权

US6042823A Enzyme composition and use thereof 有权
标题翻译：酶组成及其用途
公开(公告)号：US6042823A
公开(公告)日：2000-03-28
申请号：US340203
申请日：1999-06-28
申请人： Shigeki Kimura , Tomonari Ogawa , Kinya Kariya , Hideshi Yanase
发明人： Shigeki Kimura , Tomonari Ogawa , Kinya Kariya , Hideshi Yanase
IPC分类号： A23L1/30 , A23L1/236 , A23L1/305 , A61K31/00 , A61K38/45 , A61K38/46 , A61K38/47 , A61P1/14 , A61P3/00 , A61P3/04 , A61P3/10 , A61K38/51 , A61K38/54
CPC分类号： C12Y204/0101 , A23L27/33 , A61K38/45 , A61K38/47 , C12Y303/02011
摘要： Oligosaccharides having physiological activities are synthesized in vivo in order to, for example, improve intestinal bacterial flora. An enzyme composition comprising an enzyme capable of forming an oligosaccharide having a physiological activity in the living body and a method for forming an oligosaccharide having a physiological activity in the living body are provided.
摘要翻译：具有生理活性的寡糖在体内合成，以便例如改善肠道菌群。提供一种酶组合物，其包含能够形成在生物体内具有生理活性的寡糖的酶和在生物体中形成具有生理活性的寡糖的方法。

9. 发明授权

US5792630A Cellulose-producing microorganism transformed with a gene for an enzyme involved in sucrose metabolism 失效
标题翻译：用参与蔗糖代谢的酶的基因转化的产生纤维素的微生物
公开(公告)号：US5792630A
公开(公告)日：1998-08-11
申请号：US578536
申请日：1996-03-29
申请人： Naoto Tonouchi , Takayasu Tsuchida , Fumihiro Yoshinaga , Sueharu Horinouchi , Teruhiko Beppu , Hideshi Yanase , Takahisa Hayashi
发明人： Naoto Tonouchi , Takayasu Tsuchida , Fumihiro Yoshinaga , Sueharu Horinouchi , Teruhiko Beppu , Hideshi Yanase , Takahisa Hayashi
IPC分类号： C12N1/21 , C12N9/10 , C12P19/04 , C12N1/12 , C12N1/20 , C12N15/00
CPC分类号： C12N9/1051 , C12N9/1062 , C12P19/04 , Y10S435/823
摘要： A method for the production of a cellulosic product, which comprises: culturing a cellulose-producing microorganism transformed with a gene for an enzyme involved in sucrose metabolism in a medium containing sucrose, allowing the cellulosic product to be produced and accumulated in the medium, and collecting the cellulosic product. By the present method, the cellulosic product can be produced efficiently and economically.
摘要翻译： PCT No.PCT / JP95 / 00961 Sec。 371日期：1996年3月29日 102（e）1996年3月29日PCT PCT 1995年5月19日PCT公布。公开号WO95 / 32279 日期：1995年11月30日一种纤维素产品的制造方法，其特征在于，在含有蔗糖的培养基中，培养用参与蔗糖代谢的酶的基因转化的纤维素生成微生物，使其生产和累积纤维素产物在培养基中，并收集纤维素产品。通过本方法，可以有效且经济地制造纤维素产品。

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