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1. 发明授权

US08431336B2 Binding interactions in dipstick assays 有权
标题翻译：试纸测定中的结合相互作用
公开(公告)号：US08431336B2
公开(公告)日：2013-04-30
申请号：US10332142
申请日：2001-07-06
申请人： Helen Lee , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6832 , C12Q1/6834 , C12Q2565/519 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125
摘要： Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilized. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilized to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.
摘要翻译：描述了使用骰子来测试样品溶液中靶核酸的存在。骰子包括用于接触样品溶液的接触端和远离接触端的捕获区，捕获探针被固定到捕获区。捕获探针能够与靶核酸杂交。样品溶液与量油尺的接触端接触，并通过毛细作用行进到捕获区。如果靶核酸存在于样品溶液中，则其被捕获并且可以在捕获区域被检测。捕获探针通过间隔物固定在捕获区上。间隔物的使用增加了捕获探针和目标核酸之间相互作用的稳定性，从而提高了靶核酸检测的灵敏度。还描述了具有间隔物的检测探针。

2. 发明申请

US20120094272A1 SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES 审中-公开
标题翻译：具有多个标签的信号增强系统
公开(公告)号：US20120094272A1
公开(公告)日：2012-04-19
申请号：US13165624
申请日：2011-06-21
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： G01N33/53 , G01N33/577 , C12Q1/70 , C07K16/46 , C07K1/107
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

3. 发明申请

US20080206853A1 SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES 有权
标题翻译：具有多个标签的信号增强系统
公开(公告)号：US20080206853A1
公开(公告)日：2008-08-28
申请号：US12042762
申请日：2008-03-05
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： C12M1/00 , B01J19/00
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

4. 发明授权

US07192701B2 Capture and detection format versatility for dipstick assays 有权
标题翻译：捕捉和检测格式通用性测试仪测试
公开(公告)号：US07192701B2
公开(公告)日：2007-03-20
申请号：US10332131
申请日：2001-07-06
申请人： Helen Lee , Hsiang Yun Hu , Magda Anastassova Dineva
发明人： Helen Lee , Hsiang Yun Hu , Magda Anastassova Dineva
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6834 , C12Q1/689 , C12Q2565/625
摘要： Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.
摘要翻译：描述了用于测试样品溶液中目标核酸存在的浸渍条，浸渍条包括固定在量油尺的捕获区的通用捕获探针。通用捕获探针能够与钩状捕获探针杂交，其与样品溶液中的靶核酸杂交。将量油尺的接触端与样品溶液接触，使钩捕捉探针与目标核酸杂交，通过毛细管作用移动到可以检测靶核酸的捕获区。使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子，从而简化骰子的制备。然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。还描述了方法和试剂盒。

5. 发明授权

US09494584B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US09494584B2
公开(公告)日：2016-11-15
申请号：US13165624
申请日：2011-06-21
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： G01N33/558
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

6. 发明授权

US07972837B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US07972837B2
公开(公告)日：2011-07-05
申请号：US12042762
申请日：2008-03-05
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

7. 发明授权

US07776617B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US07776617B2
公开(公告)日：2010-08-17
申请号：US10432036
申请日：2001-11-30
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： C12Q1/00 , C12M1/34 , C12M3/00 , G01N33/53 , G01N21/00 , G01N21/75 , G01N21/76 , G01N21/77 , G01N33/566 , G01N33/543 , G01N33/551 , G01N33/544
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

8. 发明授权

US07713746B2 Dipstick assay 有权
标题翻译：试纸测定
公开(公告)号：US07713746B2
公开(公告)日：2010-05-11
申请号：US10398918
申请日：2001-10-15
申请人： Helen Lee , Magda Anastassova Dineva
发明人： Helen Lee , Magda Anastassova Dineva
IPC分类号： G01N33/53
CPC分类号： G01N33/569 , G01N33/54366 , G01N33/558 , Y02A50/54 , Y10S435/97 , Y10S436/808 , Y10S436/81 , Y10S436/823
摘要： The invention provides a dipstick and a kit comprising the dipstick, for testing for the presence of a plurality of different targets in a sample solution which comprises: a dipstick having a plurality of different capture zones and, immobilised to each capture zone, a different capture moiety, each capture moiety capable of capturing a different target; and, separately, a plurality of different detection probes, each detection probe capable of binding to a different target and each detection probe being labelled with or enabling the formation of a detection signal so that the presence of each target is indicated by the formation of a signal at the capture zone for that target; wherein the target for at least two of the capture moieties is a disease causing micro-organism or a marker indicating the existence of a disease, disorder, or condition of the host from which the sample solution was derived, and wherein at least two of the capture moieties are capable of binding to different components or markers of the same disease causing micro-organism as targets for those capture moieties. The dipsticks and kits may be used for confirmatory assays of infection by an infectious agent or disease causing microorganisms.
摘要翻译：本发明提供了一种量油尺和一个包含量油尺的试剂盒，用于测试样品溶液中多个不同靶的存在，其包括：具有多个不同捕获区的量油尺，固定在每个捕获区上的不同捕获部分，每个俘获部分能够捕获不同的靶; 并且分别地存在多个不同的检测探针，每个检测探针能够结合不同的靶，并且每个检测探针被标记或能够形成检测信号，使得每个靶的存在通过形成在该目标的捕获区域发出信号; 其中所述捕获部分中的至少两个的靶标是引起微生物或指示所述样品溶液衍生自所述宿主的疾病，病症或病症的存在的标志的疾病，并且其中至少两个捕获部分能够结合相同疾病的不同组分或标记物，导致微生物作为这些捕获部分的靶标。骰子和试剂盒可用于通过感染性物质或导致微生物的疾病的感染的确认测定。

9. 发明授权

US07186508B2 Capture and detection of target nucleic acid in dipstick assays 有权
标题翻译：在试纸测定中捕获和检测靶核酸
公开(公告)号：US07186508B2
公开(公告)日：2007-03-06
申请号：US10332134
申请日：2001-07-06
申请人： Helen Lee , Magda Anastassova Dineva , Shaun Christopher Hazlewood
发明人： Helen Lee , Magda Anastassova Dineva , Shaun Christopher Hazlewood
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6834 , G01N30/02 , G01N30/94 , C12Q2565/625 , C12Q2537/162 , B01D15/3804
摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.
摘要翻译：描述了在试纸测定中使用辅助探针。在用于测试样品溶液中目标核酸的存在的试纸测试中，样品溶液与量油尺的接触端连接，以使样品溶液与量油尺的接触端接触以引起样品溶液通过毛细管作用移动到捕获靶核酸的测试棒的捕获区域。可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。还描述了骰子和工具包。

10. 发明授权

US07867706B2 Capture and detection of target nucleic acid in dipstick assays 有权
标题翻译：在试纸测定中捕获和检测靶核酸
公开(公告)号：US07867706B2
公开(公告)日：2011-01-11
申请号：US11624861
申请日：2007-01-19
申请人： Helen Lee , Magda Anastassova Dineva , Shaun Christopher Hazlewood
发明人： Helen Lee , Magda Anastassova Dineva , Shaun Christopher Hazlewood
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6834 , G01N30/02 , G01N30/94 , C12Q2565/625 , C12Q2537/162 , B01D15/3804
摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.
摘要翻译：描述了在试纸测定中使用辅助探针。在用于测试样品溶液中目标核酸的存在的试纸测试中，将样品溶液与量油尺的接触端接触，以使样品溶液通过毛细作用移动到量油尺的捕获区，捕获靶核酸。可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。还描述了骰子和工具包。

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