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    • 3. 发明申请
    • OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS
    • 光学显微镜与光电性能光标签
    • US20080068588A1
    • 2008-03-20
    • US11944285
    • 2007-11-21
    • Harald HessRobert Betzig
    • Harald HessRobert Betzig
    • G01N1/00
    • G01N21/6428G01N21/64G01N21/6458G01N21/648G01N33/582G01N2021/6419G01N2021/6421G01N2021/6439G01N2021/6441G02B21/16G02B21/367G02B27/58
    • A method of imaging with an optical system characterized by a diffraction-limited resolution volume is disclosed. In a sample that includes a plurality of phototransformable optical labels (“PTOLs”) distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system, a first subset of the PTOLs in the sample are activated, and the density of the activated PTOLs in the first subset is less than the inverse of the diffraction-limited resolution volume. A first portion of the PTOLs in the first subset of PTOLs is excited. Radiation emitted from the activated and excited PTOLs in the first portion of PTOLs is detected with the imaging optics, and locations of activated and excited PTOLs in the first portion of PTOLs is determined with a sub-diffraction-limited accuracy based on the detected radiation emitted from the activated and excited PTOLs.
    • 公开了一种以衍射极限分辨率体积为特征的光学系统成像的方法。 在包含分布在样品中的密度大于光学系统的衍射限制分辨率体积的倒数的多个光变形光学标签(“PTOL”)的样品中,样品中的PTOL的第一子集被激活 ,并且第一子集中激活的PTOL的密度小于衍射限制分辨率体积的倒数。 PTOL的第一子集中的PTOL的第一部分被激发。 使用成像光学元件检测PTOL第一部分中激活和激发的PTOL发射的辐射,并且PTOL的第一部分中激活和激发的PTOL的位置基于检测到的辐射发射的亚衍射极限精度来确定 从激活和兴奋的PTOL。
    • 6. 发明授权
    • Method of controlling the amount of coating applied on a moving material web
    • 控制施加在移动材料网上的涂层量的方法
    • US06534113B1
    • 2003-03-18
    • US09309066
    • 1999-05-10
    • Manfred UeberschärHarald HessRudolf Münch
    • Manfred UeberschärHarald HessRudolf Münch
    • B05D312
    • B05C11/041B05C11/02D21H23/32D21H23/78
    • In a coating method for direct or indirect application of a coating medium onto a moving surface, the amount of coating medium dispensed by an applicator device within a given time period and the actuating force that is exerted on the metering element, in order to achieve a coating layer of a desired coating weight, are altered independently of each other. In a relaxation operation mode, the amount of coating medium applied within a given time period is reduced when the coating weight exceeds a desired value; when the coating weight falls below the desired value, the actuating force exerted on the metering element is reduced. In a contact pressure based operating mode, the amount of coating medium applied within a given time period is reduced when the coating weight exceeds a desired value; when the coating weight falls below the desired value the actuating force exerted on the metering element is increased. When the coating weight falls below the desired value, the amount of coating medium applied within a given time period is increased and the actuating force exerted on the metering element is reduced, while keeping constant the contact pressure of the metering element against the moving surface.
    • 在用于将涂覆介质直接或间接施加到移动表面上的涂布方法中,在给定时间段内由施加器装置分配的涂布介质的量以及施加在计量元件上的致动力,以便实现 所需涂层重量的涂层彼此独立地改变。 在弛豫操作模式中,当涂层重量超过期望值时,在给定时间段内施加的涂布介质的量减少; 当涂层重量低于期望值时,施加在计量元件上的致动力降低。 在基于接触压力的操作模式中,当涂层重量超过期望值时,在给定时间段内施加的涂布介质的量减少; 当涂层重量低于期望值时,施加在计量元件上的致动力增加。 当涂层重量低于所需值时,在给定时间段内施加的涂布介质的量增加,并且施加在计量元件上的致动力降低,同时保持计量元件抵靠运动表面的接触压力恒定。
    • 9. 发明申请
    • OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS
    • 光学显微镜与光电性能光标签
    • US20080070323A1
    • 2008-03-20
    • US11944321
    • 2007-11-21
    • Harald HessRobert Betzig
    • Harald HessRobert Betzig
    • G01N33/558
    • G01N21/6428G01N21/64G01N21/6458G01N21/648G01N33/582G01N2021/6419G01N2021/6421G01N2021/6439G01N2021/6441G02B21/16G02B21/367G02B27/58
    • An apparatus includes a position-sensitive detector to detect intensities of radiation as a function of position on the detector, and an optical system, characterized by a diffraction-limited resolution volume, adapted for imaging light emitted from activated and excited phototransformable optical labels (“PTOLs”) in a sample onto the position sensitive-detector. A first light source provides activation radiation to the sample to activate a subset of the PTOLs that are distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system. A second light source provides excitation radiation to the sample to excite a portion of the PTOLs in the subset of the PTOLs. A controller controls one both of the activation radiation and the excitation radiation provided to the sample such that a density of PTOLs in the portion of the PTOLs is less than the inverse of the diffraction-limited resolution volume.
    • 一种装置包括:位置敏感检测器,用于检测作为检测器上的位置的函数的辐射强度,以及光学系统,其特征在于衍射限制分辨率体积,其适于对从激活和激发的光变换光学标签(“ PTOL“)放置在位置敏感检测器上。 第一光源向样品提供激活辐射以激活分布在样品中的密度大于光学系统的衍射限制分辨率体积的倒数的PTOL子集。 第二光源向样品提供激发辐射以激发PTOL的子集中的PTOL的一部分。 控制器控制提供给样品的激活辐射和激发辐射之一,使得PTOL部分中的PTOL的密度小于衍射限制分辨率体积的倒数。