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2. 发明授权

US5912147A Rapid means of quantitating genomic instability 失效
标题翻译：定量基因组不稳定的快速手段
公开(公告)号：US5912147A
公开(公告)日：1999-06-15
申请号：US734973
申请日：1996-10-22
申请人： Daniel Stoler , Mark Basik , Garth Anderson
发明人： Daniel Stoler , Mark Basik , Garth Anderson
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q1/6809 , C12Q1/6844 , C12Q2600/154
摘要： Provided is a method for detecting genomic instability, independent of microsatellite alterations, comprising the steps of treating a comparison pair of genomic DNA from tumor cells and genomic DNA from normal cells with oligonucleotide primers under conditions for, and in an amplification reaction to, amplify a target sequence associated with such genomic instability. Detection of an alteration in the banding pattern of amplified product from genomic DNA from tumor cells as compared to that of normal cells, is indicative of such genomic instability. Additionally provided is a process for simultaneously assessing changes in methylation patterns in the genomic DNA tested comprising treating the genomic DNA with reagents for detecting methylation status prior to the amplification reaction, comparing the resultant banding patterns of amplified products, wherein differences in the banding patterns is indicative of an alteration in methylation. Disclosed are novel compositions for use in a method for detecting genomic instability, independent of microsatellite alterations.
摘要翻译：提供了一种用于检测基因组不稳定性的方法，与微卫星改变无关，包括以下步骤：在扩增反应条件下，扩增反应条件下，用寡核苷酸引物处理来自肿瘤细胞的基因组DNA与正常细胞的基因组DNA 与这种基因组不稳定相关的靶序列。与正常细胞相比，检测来自肿瘤细胞的基因组DNA的扩增产物的条带模式的改变指示了这种基因组不稳定性。另外提供了一种用于同时评估测试的基因组DNA中甲基化模式的变化的方法，其包括用扩增反应之前检测甲基化状态的试剂处理基因组DNA，比较所得到的扩增产物的条带模式，其中条带模式的差异为表明甲基化的改变。公开了用于检测基因组不稳定性的方法的新型组合物，与微卫星改变无关。

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