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1. 发明申请

US20050076835A1 Device for hot dip coating metal strands 失效
标题翻译：热镀金属线束装置
公开(公告)号：US20050076835A1
公开(公告)日：2005-04-14
申请号：US10503871
申请日：2003-02-20
申请人： Frank Bergmann , Michael Zielenbach , Walter Trakowski , Olaf Jepsen , Holger Behrens
发明人： Frank Bergmann , Michael Zielenbach , Walter Trakowski , Olaf Jepsen , Holger Behrens
IPC分类号： C23C2/40 , C23C2/24 , B05C3/00 , B05C5/00
CPC分类号： C23C2/24
摘要： The invention relates to a device for hot dip coating metal strands (1), particularly strip steel, in which the metal strand (1) can be vertically guided through a reservoir (3), which accommodates the molten coating metal (2), and though a guide channel (4) connected upstream therefrom. An electromagnetic inductor (5) is mounted in the area of the guide channel (4) and in order to retain the coating metal (2) inside the reservoir (3), can induce induction currents in the coating metal (2) by means of an electromagnetic traveling field. While interacting with the electromagnetic traveling field, said induction currents exert an electromagnetic force. The inductor (5) has at least two main coils (6) that are arranged in succession in movement direction (X) of the metal strand (1), and has at least two correction coils (7) for controlling the position of the metal strand (1) inside the guide channel (4) in direction (N), which is normal to the surface of the metal strand (1). These correction coils are also arranged in succession in movement direction (X) of the metal strand (1). In order to improve the efficiency of the control of the metal strip inside the guide channel, the invention provides that at least a portion of the correction coils (7), when viewed in movement direction (X) of the metal strand (1), are arranged so that they are offset with regard to one another perpendicular to movement direction (X) and perpendicular to direction (N) that is normal to the surface of the metal strand (1).
摘要翻译：本发明涉及一种用于热浸镀金属线（1），特别是带钢的装置，其中金属线（1）可以垂直地引导通过容纳熔融涂层金属（2）的储存器（3），以及尽管其上游连接有引导通道（4）。电磁感应器（5）安装在引导通道（4）的区域中，为了将涂层金属（2）保持在储存器（3）内部，可以通过以下方式引起涂层金属（2）中的感应电流：电磁行驶场。当与电磁行进场相互作用时，所述感应电流施加电磁力。电感器（5）具有至少两个主要线圈（6），它们沿金属线束（1）的移动方向（X）连续布置，并且具有至少两个用于控制金属线的位置的校正线圈（7）在导向通道（4）内的方向（N）上的线（1），其垂直于金属线（1）的表面。这些校正线圈也在金属线（1）的移动方向（X）上相继排列。为了提高引导通道内的金属带的控制效率，本发明提供了当从金属线（1）的移动方向（X）观察时，至少一部分校正线圈（7）被布置成使得它们相对于垂直于运动方向（X）并且垂直于垂直于金属线（1）的表面的方向（N）彼此偏移。

2. 发明授权

US06929697B2 Device for hot dip coating metal strands 失效
标题翻译：热镀金属线束装置
公开(公告)号：US06929697B2
公开(公告)日：2005-08-16
申请号：US10503871
申请日：2003-02-20
申请人： Frank Bergmann , Michael Zielenbach , Walter Trakowski , Olaf Norman Jepsen , Holger Behrens
发明人： Frank Bergmann , Michael Zielenbach , Walter Trakowski , Olaf Norman Jepsen , Holger Behrens
IPC分类号： C23C2/40 , C23C2/24 , B05C3/12
CPC分类号： C23C2/24
摘要： The invention relates to a device for hot dip coating metal strand (1), particularly strip steel, in which the metal strand (1) can be vertically guided through a reservoir (3), which accommodates the molten coating metal (2), and though a guide channel (4) connected upstream therefrom. An electromagnetic inductor (5) is mounted in the area of the guide channel (4) and in order to retain the coating metal (2) inside the reservoir (3), can induce induction currents in the coating metal (2) by an electromagnetic traveling field. While interacting with the electromagnetic traveling field, the induction currents exert an electromagnetic force. The inductor (5) has at least two main coils (6) that are arranged in succession in movement direction (X) of the metal strand (1), and has at least two correction coils (7) for controlling the position of the metal strand (1) inside the guide channel (4) in direction (N), which is normal to the surface of the metal strand (1). These correction coils are also arranged in succession in movement direction (X) of the metal strand (1). In order to improve the efficiency of the control of the metal strip inside the guide channel, the invention provides that at least a portion of the correction coils (7), when viewed in movement direction (X) of the metal strand (1), are arranged so that they are offset with regard to one another perpendicular to movement direction (X) and perpendicular to direction (N) that is normal to the surface of the metal strand (1).
摘要翻译：本发明涉及一种用于热浸镀金属线（1），特别是带钢的装置，其中金属股线（1）可以垂直地引导通过容纳熔融涂层金属（2）的储存器（3），以及尽管其上游连接有引导通道（4）。电磁感应器（5）安装在引导通道（4）的区域中，为了将涂层金属（2）保持在储存器（3）内部，可以通过电磁感应器（2）诱导涂层金属（2）中的感应电流旅游领域。当与电磁行进场相互作用时，感应电流施加电磁力。电感器（5）具有至少两个主要线圈（6），它们沿金属线束（1）的移动方向（X）连续布置，并且具有至少两个用于控制金属线的位置的校正线圈（7）在导向通道（4）内的方向（N）上的线（1），其垂直于金属线（1）的表面。这些校正线圈也在金属线（1）的移动方向（X）上相继排列。为了提高引导通道内的金属带的控制效率，本发明提供了当从金属线（1）的移动方向（X）观察时，至少一部分校正线圈（7）被布置成使得它们相对于垂直于运动方向（X）并且垂直于垂直于金属线（1）的表面的方向（N）彼此偏移。

3. 发明授权

US08350038B2 Fluorescence quencher molecules 有权
标题翻译：荧光猝灭剂分子
公开(公告)号：US08350038B2
公开(公告)日：2013-01-08
申请号：US12898107
申请日：2010-10-05
申请人： Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
发明人： Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
IPC分类号： C07D217/22 , C07D217/00
CPC分类号： C07D401/04
摘要： Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET).
摘要翻译：公开了吡啶基 - 异喹啉二酮衍生物，制备这些衍生物的方法，包含吡啶基 - 异喹啉二酮衍生物的共轭物和（i）固体支持物，或（ii）生物分子，制备这些缀合物的方法以及这些共轭物作为荧光共振能量转移（FRET）中的猝灭剂。

4. 发明申请

US20090208930A1 Dna decontamination method 有权
标题翻译： Dna去污方法
公开(公告)号：US20090208930A1
公开(公告)日：2009-08-20
申请号：US11658569
申请日：2005-09-02
申请人： Frank Bergmann , Christine Markert-Hahn
发明人： Frank Bergmann , Christine Markert-Hahn
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要： The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
摘要翻译：本发明涉及一种用于扩增样品中的甲基化靶核酸的方法，同时避免通过使其失活来扩增非甲基化靶核酸。这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。

5. 发明申请

US20060058518A1 Method for bisulfite treatment 有权
标题翻译：亚硫酸氢盐处理方法
公开(公告)号：US20060058518A1
公开(公告)日：2006-03-16
申请号：US10540406
申请日：2004-01-28
申请人： Frank Bergmann , Christine Market-Hahn , Joerg Kleiber , Dirk Block
发明人： Frank Bergmann , Christine Market-Hahn , Joerg Kleiber , Dirk Block
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.
摘要翻译：本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基在核酸中进行脱氨。然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。

6. 发明授权

US06235476B1 Process for detecting nucleic acids by mass determination 有权
标题翻译：通过质量测定检测核酸的方法
公开(公告)号：US06235476B1
公开(公告)日：2001-05-22
申请号：US09242536
申请日：1999-03-17
申请人： Frank Bergmann , Rupert Herrmann , Uwe Kobold
发明人： Frank Bergmann , Rupert Herrmann , Uwe Kobold
IPC分类号： C12Q168
CPC分类号： C12Q1/6872 , C12Q1/6827 , Y10T436/143333
摘要： A method for detecting nucleic acids by binding a probe P to a partial sequence S contained in the nucleic acid to produce a binding product B1, degrading the nucleic acid to produce a binding product B2 containing a partial nucleic acid F of a defined length and detecting the binding product B2 or the partial nucleic acid F a part based on its mass is particularly suitable for the parallel detection of nucleic acid of different sequences.
摘要翻译：通过将探针P与核酸中所含的部分序列S结合以产生结合产物B1来检测核酸的方法，使核酸降解以产生含有限定长度的部分核酸F的结合产物B2并检测结合产物B2或部分核酸F基于其质量的部分特别适用于不同序列的核酸的平行检测。

7. 发明授权

US5962540A One-component or two-component reactive adhesive 失效
标题翻译：单组分或双组分反应性粘合剂
公开(公告)号：US5962540A
公开(公告)日：1999-10-05
申请号：US776728
申请日：1997-03-03
申请人： Carsten Friese , Frank Bergmann , Thomas Huver
发明人： Carsten Friese , Frank Bergmann , Thomas Huver
IPC分类号： C09J9/00 , C08G18/67 , C08J9/10 , C08L75/16 , C09J4/00 , C09J4/02 , C09J5/08 , C09J11/06 , C09J133/04 , C09J175/00 , C09J175/16 , C09J201/00 , H01F27/32 , C08J9/02
CPC分类号： C08J9/102 , C08G18/672 , C09J175/16 , C09J4/00 , C09J5/08 , C08J2207/02 , C08J2375/16 , C08L75/16 , C09J2475/00
摘要： A reaction adhesive becomes porous after hardening. This allows both small and larger gaps to be sealed between the bonded substrates, as the liquid adhesive has a low to medium viscosity and the hardened glue shows a marked volume increase. Reaction adhesives based on (meth)acrylates and that contain azo-bis-isobutyronitrile as initiator and expanding agent are preferably used. The disclosed reaction adhesive is preferably used for gluing reactive coils.
摘要翻译： PCT No.PCT / EP95 / 02961 Sec。 371日期1997年3月3日 102（e）1997年3月3日PCT PCT 1995年7月25日PCT公布。公开号WO96 / 04347 日期1996年2月15日硬化后反应粘合剂变多孔。这允许在粘合的基底之间密封小的和较大的间隙，因为液体粘合剂具有低至中等的粘度，并且硬化的胶显示出显着的体积增加。优选使用基于（甲基）丙烯酸酯并含有偶氮双异丁腈作为引发剂和发泡剂的反应粘合剂。所公开的反应粘合剂优选用于胶合反应性线圈。

8. 发明授权

US08877464B2 Qualitative and quantitative detection of microbial nucleic acids 有权
标题翻译：定性和定量检测微生物核酸
公开(公告)号：US08877464B2
公开(公告)日：2014-11-04
申请号：US13191700
申请日：2011-07-27
申请人： Reiner Babiel , Frank Bergmann , Dorothea Johanna Sizmann
发明人： Reiner Babiel , Frank Bergmann , Dorothea Johanna Sizmann
IPC分类号： C12P19/34 , C12Q1/68 , G01N21/64
CPC分类号： C12Q1/686 , C12P19/34 , C12Q1/68 , C12Q1/6851 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses.
摘要翻译：本发明涉及使用至少第一对照核酸或不同浓度的第一和第二对照核酸定性和定量检测微生物核酸的新方法和用途。该方法基于核酸的扩增，例如聚合酶链反应。还提供了包括用于执行所述方法和用途的组件的套件。

9. 发明授权

US08445206B2 Set of oligonucleotide probes as well as methods and uses thereto 有权
标题翻译：一组寡核苷酸探针及其方法和用途
公开(公告)号：US08445206B2
公开(公告)日：2013-05-21
申请号：US13361549
申请日：2012-01-30
申请人： Frank Bergmann , Walter Eberle , Thomas Fischer , Herbert von der Eltz
发明人： Frank Bergmann , Walter Eberle , Thomas Fischer , Herbert von der Eltz
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译：本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。

10. 发明授权

US08124338B2 Use of TDE for isolation of nucleic acids 有权
标题翻译：使用TDE分离核酸
公开(公告)号：US08124338B2
公开(公告)日：2012-02-28
申请号：US12481807
申请日：2009-06-10
申请人： Horst Donner , Frank Bergmann , Nina Lassonczyk , Manfred Watzele , Marcus Schmid
发明人： Horst Donner , Frank Bergmann , Nina Lassonczyk , Manfred Watzele , Marcus Schmid
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1006
摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译：本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。为此，本发明还提供了包含TDE的组合物。方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。特别地，本发明包括用于纯化低分子量核酸的方法。通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

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