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1. 发明授权

US08268596B2 Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US08268596B2
公开(公告)日：2012-09-18
申请号：US12815272
申请日：2010-06-14
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： A61K39/00 , A61K39/095 , C12P10/18 , C12N9/10
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal β1→4 to GlcNAc or Glc; adding GalNAc or GlcNAc β1→3 to Gal; and adding Gal αl→4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Galβ1→4GlcNAcβ1→3Galβ1→4Glc (a mimic of lacto-N-neotetraose), GalNAcβ1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcβ1→4 (a mimic ganglioside), and Galα1→4Galβ1→4Glcβ1→4Hep→R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Gal和bgr; 1→4; 将GalNAc或GlcNAc＆bgr; 1→3加入Gal; 并将Galα1→4加到Gal。本发明的糖基转移酶特别适用于合成寡糖Gal＆bgr; 1→4GlcNAc＆bgr; 1→3Gal＆bgr; 1→4Glc（模拟乳-N-新四糖），GalNAc＆bgr; 1→3Gal＆bgr; 1→4GlcNAc＆bgr; 1→ 3Gal＆bgr; 1→4Glc＆bgr; 1→4（模拟神经节苷脂），Galα1→4Gal＆bgr; 1→4Glc＆bgr; 1→4Hep→R（模拟糖尿糖的糖部分）。

2. 发明授权

US06342382B1 Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 有权
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US06342382B1
公开(公告)日：2002-01-29
申请号：US09333412
申请日：1999-06-15
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12P2106
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal &bgr;1→4 to GlcNAc or Glc; adding GalNAc or GlcNAc &bgr;1→3 to Gal; and adding Gal &agr;1→4 to Gal. The glycosyltansferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal&bgr;1→4GlcNAc&bgr;1→3Gal&bgr;1→4Glc (a mimic of lacto-N-neotetraose), GalNAc&bgr;1→3Gal&bgr;1→4GlcNAc&bgr;1→3Gal&bgr;1→4Glc&bgr;1→4 (a mimic ganglioside), and Gal&agr;1→4Gal&bgr;1→4Glc&bgr;1→4Hep→R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于寡糖Galbeta1→4GlcNAcβ1→3Galβ1→4Glc（乳-N-新四糖的模拟物），GalNAcbeta1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcbeta1→ 4（模拟神经节苷脂）和Galalpha1->4Galβ1→4Glcbeta1-> 4Hep-> R（模拟糖尿糖的糖部分）。

3. 发明授权

US06780624B2 Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US06780624B2
公开(公告)日：2004-08-24
申请号：US10007267
申请日：2001-12-03
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12N900
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal &bgr;1→4 to GlcNAc or Glc; adding GalNAc or GlcNAc &bgr;1→3 to Gal; and adding Gal &agr;1→4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal&bgr;1→4GlcNAc&bgr;1→3Gal&bgr;1→4Glc (a mimic of lacto-N-neotetraose), GalNAc&bgr;1→3Gal&bgr;1→4GlcNAc&bgr;1→3Gal&bgr;1→4Glc&bgr;1→4 (a mimic ganglioside), and Gal&agr;1→4Gal&bgr;1→4Glc&bgr;1→4Hep→R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于寡糖Galbeta1→4GlcNAcβ1→3Galβ1→4Glc（乳-N-新四糖的模拟），GalNAcβ1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcbeta1→ 4（模拟神经节苷脂）和Galalpha1->4Galβ1→4Glcbeta1-> 4Hep-> R（模拟糖尿糖的糖部分）。

4. 发明授权

US5798233A Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US5798233A
公开(公告)日：1998-08-25
申请号：US683458
申请日：1996-07-18
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12N15/09 , A61K35/74 , A61K39/095 , A61P31/04 , C07K14/22 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12R1/36
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc .beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.l.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于低聚糖Galβ1-> 4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNAcβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分的糖尿病）。

5. 发明授权

US5705367A Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US5705367A
公开(公告)日：1998-01-06
申请号：US683426
申请日：1996-07-18
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12N15/09 , A61K35/74 , A61K39/095 , A61P31/04 , C07K14/22 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12R1/36
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta..fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ→4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于合成低聚糖Galβ1-> 4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNAcβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分的糖尿病）。

6. 发明授权

US5945322A Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US5945322A
公开(公告)日：1999-08-31
申请号：US878360
申请日：1997-06-18
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12N15/09 , A61K35/74 , A61K39/095 , A61P31/04 , C07K14/22 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12R1/36 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.l.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于合成低聚糖Galβ1-> 4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNAcβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分的糖尿病）。

7. 发明授权

US5545553A Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them 失效
标题翻译：用于寡糖生物合成的糖基转移酶和编码它们的基因
公开(公告)号：US5545553A
公开(公告)日：1996-08-13
申请号：US312387
申请日：1994-09-26
申请人： Emil C. Gotschlich
发明人： Emil C. Gotschlich
IPC分类号： C12N15/09 , A61K35/74 , A61K39/095 , A61P31/04 , C07K14/22 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12R1/36 , C12P19/00
CPC分类号： C07K14/22 , C12N9/1048 , C12N9/1051 , C12P19/18
摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNac.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).
摘要翻译：本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。本发明的糖基转移酶特别适用于合成低聚糖Galβ1->4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNacβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分的糖尿病）。

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