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1. 发明申请

US20050003408A1 Libraries of oligomers labeled with different tags 失效
标题翻译：用不同标签标记的低聚物的文库
公开(公告)号：US20050003408A1
公开(公告)日：2005-01-06
申请号：US10843873
申请日：2004-05-11
申请人： Edwin Southern , Mikhail Shchepinov , John Housby , Alan Hamilton , John Elder
发明人： Edwin Southern , Mikhail Shchepinov , John Housby , Alan Hamilton , John Elder
IPC分类号： G01N27/62 , C07B61/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B20/04 , C40B30/04 , C40B30/10 , C40B40/06 , C40B40/10 , C40B50/10 , C40B60/10 , C40B70/00 , G01N33/483 , G01N33/50 , C12P19/34
CPC分类号： C12Q1/6872 , C07H21/00 , C40B20/04 , C40B30/04 , C40B30/10 , C40B40/06 , C40B40/10 , C40B50/10 , C40B50/16 , C40B60/10 , C40B70/00
摘要： A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels is and their use in assays and nucleic acid analysis methods.
摘要翻译：通过使用优选颗粒载体制备一组标记化合物的方法包括将载体分成批次，在每批载体上进行不同的化学反应。将化学部分连接到许多支持物，用不同的标签标记每批支持物的一小部分，并且组合所述许多支持物。重复这些步骤数次，优选地构建携带标记物的标签的低聚物分子，其标识低聚物的单体单元的性质和位置，并且其可从载体上释放。优选的标记物，可通过裂解从化合物中释放以提供用于通过质谱分析的带电基团，是三苯甲基（三甲基苯基）家族的组。还要求保护的是这些标记的文库是它们在测定和核酸分析方法中的用途。

2. 发明申请

US20060022129A1 Peptide mass spectrometry rich in daughter ions 审中-公开
标题翻译：肽质谱富含子离子
公开(公告)号：US20060022129A1
公开(公告)日：2006-02-02
申请号：US11136436
申请日：2005-05-25
申请人： Mikhail Shchepinov , Susan Wheeler , Edwin Southern
发明人： Mikhail Shchepinov , Susan Wheeler , Edwin Southern
IPC分类号： B01D59/44
CPC分类号： G01N33/6851 , G01N33/6848 , G01N2458/15
摘要： Methods of analysing peptides by mass spectrometry are disclosed. In particular, methods of analysing peptides by fragmentation mass spectrometry (MSn, where n is at least 2) are disclosed. The methods involve derivatisation of a peptide at its N-terminus such that peaks corresponding to both a and b (and y) daughter ions are identifiable in fragmentation mass spectra of the derivatised peptide. The fragmentation mass spectra of the derivatised peptide contain additional information (relative to the fragmentation mass spectra of the underivatised peptide) useful for determination of the amino acid sequence of the peptide.
摘要翻译：公开了通过质谱分析肽的方法。特别地，公开了通过碎片质谱法（MS＆lt; n＆gt;，其中n至少为2）分析肽的方法。所述方法涉及在其N末端衍生肽，使得对应于a和b（和y）子离子的峰可在衍生肽的断裂质谱中鉴定。衍生肽的断裂质谱包含可用于测定肽的氨基酸序列的附加信息（相对于未衍生肽的断裂质谱）。

3. 发明申请

US20050043414A1 Trityl-type compounds and their use 失效
标题翻译：三苯甲基型化合物及其用途
公开(公告)号：US20050043414A1
公开(公告)日：2005-02-24
申请号：US10937545
申请日：2004-09-08
申请人： Mikhail Shchepinov , Edwin Southern
发明人： Mikhail Shchepinov , Edwin Southern
IPC分类号： G01N21/64 , C07C235/42 , C07H21/00 , C09B3/14 , C09B11/06 , C09K11/06 , C12Q1/68 , G01N21/78 , G01N33/50 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/58 , A61K31/495 , A61K31/03
CPC分类号： C07H21/00 , C09B3/14 , C09B11/06 , G01N33/542
摘要： The present invention pertains to trityl-type compounds comprising, bonded to the same atom, three aryl groups, of which at least one is a fluorophore and at least one has a substituent including a functional group, and wherein the compound can exist in a non-ionised state or in an ionised state conjugated with the aryl groups.
摘要翻译：本发明涉及三苯甲基型化合物，其包含与相同原子键合的三个芳基，其中至少一个是荧光团，至少一个具有包含官能团的取代基，并且其中该化合物可以以非 - 或与芳基共轭的电离状态。

4. 发明授权

US5770367A Tag reagent and assay method 失效
标题翻译：标签试剂和测定方法
公开(公告)号：US5770367A
公开(公告)日：1998-06-23
申请号：US586875
申请日：1996-02-05
申请人： Edwin Southern , William Jonathan Cummins
发明人： Edwin Southern , William Jonathan Cummins
IPC分类号： G01N27/62 , C07F7/18 , C07H21/00 , C07H21/04 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/58 , G01N33/68 , C07H19/00 , C07H21/02
CPC分类号： C12Q1/6874 , C07F7/1856 , C07H21/00 , C12N15/1075 , C12Q1/6816 , C12Q1/6837 , C12Q1/6872 , Y10S435/912
摘要： A reagent comprises: a) an analyte moiety comprising at least two analyte residues, and linked to; b) a tag moiety comprising one or more reporter groups adapted for detection by mass spectrometry, wherein a reporter group designates analyte residue, and the reporter group at each position of the tag moiety is chosen to designate an analyte residue at a defined position of the analyte moiety. A plurality of such reagents, each comprising a different analyte moiety, provides a library of reagents which may be used in assay methods involving a target substance. Analysis of the tag moieties indicates the nature of the analyte moieties bound to the target substance. A method of sequencing nucleic acid employs a library of the reagents to determine the sequence of a target nucleic acid.
摘要翻译： PCT No.PCT / GB94 / 01675 Sec。 371日期：1996年2月5日 102（e）日期1996年2月5日PCT 1994年8月1日PCT公布。出版物WO95 / 04160 日期：1995年2月9日试剂包括：a）包含至少两个分析物残基并与之连接的分析物部分; b）包含一个或多个适于通过质谱检测的报告基团的标签部分，其中报告基团指定分析物残基，并且选择标签部分的每个位置处的报告基团来表示在所述标记部分的限定位置处的分析物残基分析物部分。多个这样的试剂，各自包含不同的分析物部分，提供可用于涉及靶物质的测定方法的试剂文库。标签部分的分析表明结合到目标物质的分析物部分的性质。测序核酸的方法使用试剂文库来确定靶核酸的序列。

5. 发明授权

US09587278B2 Combined CGH and allele specific hybridisation method 有权
标题翻译：组合CGH和等位基因特异性杂交方法
公开(公告)号：US09587278B2
公开(公告)日：2017-03-07
申请号：US13520935
申请日：2011-01-07
申请人： Douglas Hurd , Edwin Southern
发明人： Douglas Hurd , Edwin Southern
IPC分类号： C12Q1/68
CPC分类号： G06F19/20 , C12Q1/6837 , C12Q1/6883 , C12Q1/6886 , C12Q2600/156 , C12Q2600/172 , C12Q2525/151 , C12Q2535/131 , C12Q2537/16 , C12Q2539/115 , C12Q2565/513
摘要： The invention combines the fields of comparative genomic hybridization (CGH) analysis and SNP array analysis. It relates to methods for detecting and mapping genetic abnormalities associated with various diseases. In particular the invention provides a method for simultaneously performing array CGH and SNP array analysis on a genomic DNA sample comprising contacting a nucleic acid array which comprises a first probe set and a second probe set with a genomic DNA sample, comprising a test and reference sample, under hybridization conditions, comparing the amount of test sample and reference sample hybridized to the hybridization probes of the first probe set, comparing the amount of test sample and reference sample hybridized to the hybridization probes of the second probe set; and using the data obtained to determine the copy number of at least one locus; and at least one SNP in the genomic DNA sample.
摘要翻译：本发明结合了比较基因组杂交（CGH）分析和SNP阵列分析的领域。它涉及检测和绘制与各种疾病相关的遗传异常的方法。特别地，本发明提供了一种用于在基因组DNA样品上同时进行阵列CGH和SNP阵列分析的方法，包括使包含第一探针组和第二探针组的核酸阵列与基因组DNA样品接触，所述核酸阵列包含测试和参考样品在杂交条件下，比较与第一探针组的杂交探针杂交的测试样品和参考样品的量，比较与第二探针组的杂交探针杂交的测试样品和参考样品的量; 并使用获得的数据来确定至少一个轨迹的拷贝数; 和基因组DNA样品中的至少一个SNP。

6. 发明申请

US20050266408A1 Method for analysing a polynucleotide 有权
标题翻译：分析多核苷酸的方法
公开(公告)号：US20050266408A1
公开(公告)日：2005-12-01
申请号：US10701605
申请日：2003-11-06
申请人： Edwin Southern , Clare Pritchard , Stephen Case-Green
发明人： Edwin Southern , Clare Pritchard , Stephen Case-Green
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/6858 , B01J19/0046 , B01J2219/00608 , B01J2219/0061 , B01J2219/00626 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6862 , C12Q1/6869 , C12Q1/6876 , C12Q2563/179 , C12Q2600/156 , C12Q2521/501 , C12Q2565/501 , C12Q2535/125 , C12Q2537/125 , C12Q2533/107 , C12Q2525/151 , C12Q2565/518 , C12Q2521/101 , C12Q2565/537 , C12Q2561/125
摘要： A method of analysing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilised oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analysed. Arrays of immobilised oligonucleotides are provided for use in the method.
摘要翻译：分析多核苷酸靶的方法包括用寡核苷酸探针（通常是固定的寡核苷酸探针的阵列）孵育靶，以形成双链体，并使用连接酶或聚合酶延伸双链体的一条链。可以分析点突变或可变数串联重复部分。提供固定化寡核苷酸的阵列用于该方法。

7. 发明申请

US20050032048A1 ANALYZING POLYNUCLEOTIDE SEQUENCES 审中-公开
标题翻译：分析多核苷酸序列
公开(公告)号：US20050032048A1
公开(公告)日：2005-02-10
申请号：US09422803
申请日：1999-10-22
申请人： Edwin Southern
发明人： Edwin Southern
IPC分类号： B01J19/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6883 , B01J19/0046 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00385 , B01J2219/0043 , B01J2219/00527 , B01J2219/00529 , B01J2219/00574 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q2600/156 , C40B40/06 , C40B60/14 , C12Q2565/507 , C12Q2535/131 , C12Q2525/204 , C12Q2565/513
摘要： Abstract of the Disclosure This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pairs of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.
摘要翻译：发明内容本发明提供了一种用于分析多核苷酸序列的装置和方法; 未知序列或已知序列。支持，例如玻璃板载有能够参与杂交反应的完整的寡核苷酸组的全部阵列或选定部分的阵列。阵列可以包括一对或多对选定长度的寡核苷酸。多核苷酸序列或其片段在杂交条件下被标记并应用于阵列。应用包括已知点突变，基因组指纹图谱，连锁分析，mRNAs表征，mRNA群体和序列测定的分析。

8. 发明授权

US5700637A Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays 失效
标题翻译：用于分析多核苷酸序列的装置和方法以及产生寡核苷酸阵列的方法
公开(公告)号：US5700637A
公开(公告)日：1997-12-23
申请号：US230012
申请日：1994-04-19
申请人： Edwin Southern
发明人： Edwin Southern
IPC分类号： B01J19/00 , C12Q1/68 , C40B40/06 , C40B60/14 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00385 , B01J2219/0043 , B01J2219/00527 , B01J2219/00529 , B01J2219/00574 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00641 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.
摘要翻译：本发明提供了一种用于分析多核苷酸序列的装置和方法; 未知序列或已知序列。支持，例如玻璃板载有能够参与杂交反应的完整的寡核苷酸组的全部阵列或选定部分的阵列。该阵列可以包括一个或多个选定长度的寡核苷酸对。多核苷酸序列或其片段在杂交条件下被标记并应用于阵列。应用包括已知点突变，基因组指纹图谱，连锁分析，mRNA表征，mRNA群体和序列测定的分析。

9. 发明授权

US07888494B2 Analysing polynucleotide sequences 失效
标题翻译：分析多核苷酸序列
公开(公告)号：US07888494B2
公开(公告)日：2011-02-15
申请号：US10772467
申请日：2004-02-06
申请人： Edwin Southern
发明人： Edwin Southern
IPC分类号： C07H21/02 , C07H21/04 , C12M1/18 , C12Q1/68
CPC分类号： C12Q1/6876 , B01J19/0046 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00385 , B01J2219/0043 , B01J2219/00527 , B01J2219/00529 , B01J2219/00574 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C40B40/06 , C40B60/14 , C12Q2565/513 , C12Q2565/507 , C12Q2525/204 , C12Q2565/501
摘要： An apparatus is provided for analysing a polynucleotide. The apparatus includes: a support having an impermeable surface; porous material attached to the impermeable surface; and an array of oligonucleotides with predetermined sequences attached to the porous material. The array includes at least two defined cells, the sequence of the oligonucleotides of a first cell being different from the sequence of the oligonucleotides of a second cell, and the oligonucleotides being shorter than the polynucleotide.
摘要翻译：提供了用于分析多核苷酸的装置。该装置包括：具有不透水表面的支撑件; 附着在不渗透表面上的多孔材料; 以及具有连接到多孔材料的预定序列的寡核苷酸阵列。该阵列包括至少两个限定的细胞，第一个细胞的寡核苷酸的序列不同于第二个细胞的寡核苷酸的序列，并且该寡核苷酸比该多核苷酸短。

10. 发明申请

US20090098541A1 Devices and processes for analysing individual cells 失效
标题翻译：用于分析单个单元的设备和过程
公开(公告)号：US20090098541A1
公开(公告)日：2009-04-16
申请号：US11919761
申请日：2006-05-03
申请人： Edwin Southern , Wouter Meuleman , Dietrich Wilhelm Karl Lueerssen , Natalie Milner
发明人： Edwin Southern , Wouter Meuleman , Dietrich Wilhelm Karl Lueerssen , Natalie Milner
IPC分类号： C12Q1/68 , C12M1/00 , B29C39/00 , C12Q1/02
CPC分类号： C12Q1/6876 , B01L3/5025 , B01L3/502715 , B01L3/502753 , B01L2200/027 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , C12M47/06 , G01N2015/1006
摘要： A device for individually analysing cells of interest, comprising (a) a channel for receiving the contents of a cell of interest, wherein the channel has an input end and an output end, and (b) a cell trapping site in proximity to the input end of the channel, wherein (i) the input end of the channel is adapted such that an intact cell of interest cannot enter the channel; and (ii) the channel contains one or more analytical components for analysing the contents of the cell of interest. In use, a cell is applied to the device, where it is trapped by the cell trapping means. The cell cannot enter the channel intact, but its contents can be released in situ to enter the channel's input end. The contents can then move down the channel, towards the output end, and they encounter the immobilised reagents, thereby permitting analysis of the cell contents.
摘要翻译：一种用于单独分析感兴趣的单元的设备，包括：（a）用于接收感兴趣单元的内容的通道，其中所述通道具有输入端和输出端，以及（b）靠近输入端的单元陷阱位置其中（i）信道的输入端被适配成使得感兴趣的完整小区不能进入信道; 和（ii）该通道包含用于分析感兴趣细胞的内容物的一个或多个分析成分。在使用中，将细胞施加到装置，在其中被细胞俘获装置捕获。单元格无法完整进入通道，但其内容可以原位释放进入通道的输入端。然后，内容物可以向下移动通道，朝向输出端，并且它们遇到固定的试剂，从而允许细胞内容物的分析。

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