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    • 4. 发明申请
    • Pongamia Genetic Markers and Method of Use
    • Pongamia遗传标记和使用方法
    • US20140053294A1
    • 2014-02-20
    • US13885886
    • 2011-11-18
    • Peter M. Gresshoff
    • Peter M. Gresshoff
    • C12Q1/68C07H21/04
    • C12Q1/6895C07H21/04C12Q2600/13C12Q2600/156
    • Primers suitable for nucleic acid sequence amplification of Pongamia plant genetic markers and a method of genetic analysis of Pongamia plants are provided. The primers comprise a repeat unit of two or three nucleotides repeated five to ten times together with a three prime extension of two or three nucleotides. Genetic markers amplified by the primers are also provided, from which may be produced further primers for genetic analysis of Pongamia plants. The primers, genetic markers and methods of genetic analysis may be suitable for selection and breeding of Pongamia plants having desired traits such as, or relating to, seed size, seed number, seed oil content, seed oil quality, seed flavour and toxicity, disease resistance, water use efficiency, nitrogen use efficiency, precocious flowering, flowering time, tree size, tree shape, growth rate, drought tolerance, salinity tolerance and/or growth in low-nutrient soils.
    • 提供了适用于Pongamia植物遗传标记的核酸序列扩增的引物和Pongamia植物的遗传分析方法。 引物包含两个或三个核苷酸的重复单元重复五到十次,连同两个或三个核苷酸的三次延伸。 还提供了通过引物扩增的遗传标记,从中可以制备用于Pongamia植物的遗传分析的另外的引物。 引物,遗传标记和遗传分析方法可适用于具有所需性状或与种子大小,种子数,种子油含量,种子油质量,种子风味和毒性,疾病相关的所需性状的Pongamia植物的选择和育种 耐旱性,水分利用效率,氮利用效率,早熟开花,开花时间,树木大小,树形状,生长速率,耐旱性,耐盐性和/或低营养土壤的生长。
    • 5. 发明授权
    • Method for profiling nucleic acids of unknown sequence using arbitrary
oligonucleotide primers
    • 使用任意寡核苷酸引物分析未知序列核酸的方法
    • US5413909A
    • 1995-05-09
    • US6380
    • 1993-01-19
    • Brant J. BassamGustavo Caetano-AnollesPeter M. Gresshoff
    • Brant J. BassamGustavo Caetano-AnollesPeter M. Gresshoff
    • C12N15/09C12N15/10C12Q1/68
    • C12Q1/6858C12N15/10C12Q1/686
    • A method for amplifying nucleic acid sequences in a template comprising at least one nucleic acid or mixture of nucleic acids wherein each nucleic acid comprises at least one strand. The method comprises the steps of treating the template with at least one oligonucleotide primer having a nucleic acid sequence that is substantially complementary to at least one nucleic acid sequence on the template under conditions such that the primer will bind to a segment that is substantially complementary to the primer, producing primed strands of nucleic acid. The primed strands produce extension strands, wherein the extension strands comprise the primer in combination with a sequence of nucleic acids that is substantially complementary to the template in a 5' to 3' direction from the primer. The extension strands are further treated with the primer producing amplification strands which are treated with primer. The amplification strands are repeatedly treated with primer to produce large numbers of amplification strands whereby the nucleic acid sequences of the amplification strands are substantially similar to nucleic acid sequences in the original template.
    • 一种用于扩增模板中的核酸序列的方法,其包含至少一种核酸或核酸混合物,其中每个核酸包含至少一个链。 该方法包括以下步骤:用至少一种寡核苷酸引物处理模板,所述寡核苷酸引物具有与模板上至少一个核酸序列基本上互补的条件,使得引物将结合至基本上与 引物,产生引发的核酸链。 引发的链产生延伸链,其中延伸链包含与来自引物的5'至3'方向上的模板基本上互补的核酸序列的引物。 延伸链进一步用引物产生的引物处理的引物进行处理。 用引物重复处理扩增链以产生大量的扩增链,由此扩增链的核酸序列基本上与原始模板中的核酸序列相似。