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1. 发明授权

US08420370B2 Alpha-amylase variants 有权
公开(公告)号：US08420370B2
公开(公告)日：2013-04-16
申请号：US13355047
申请日：2012-01-20
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

2. 发明授权

US08465957B2 Alpha-amylase variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US08465957B2
公开(公告)日：2013-06-18
申请号：US13478512
申请日：2012-05-23
申请人： Allan Svendsen , Soren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
发明人： Allan Svendsen , Soren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
IPC分类号： C12N9/28
CPC分类号： C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.
摘要翻译：本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。这些变体在高温下（相对于亲本）具有增加的稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

3. 发明申请

US20090203109A1 Alpha-Amylase Variants 审中-公开
标题翻译： α-淀粉酶变体
公开(公告)号：US20090203109A1
公开(公告)日：2009-08-13
申请号：US12417453
申请日：2009-04-02
申请人： Allan Svendsen , Soren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
发明人： Allan Svendsen , Soren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
IPC分类号： C12N9/28
CPC分类号： C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要： The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.
摘要翻译：本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。这些变体在高温下（相对于亲本）具有增加的稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

4. 发明授权

US08124395B2 Alpha-amylase variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US08124395B2
公开(公告)日：2012-02-28
申请号：US12952695
申请日：2010-11-23
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/00 , C12N9/28 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

5. 发明授权

US07306936B2 Alpha-amylase variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US07306936B2
公开(公告)日：2007-12-11
申请号：US10146327
申请日：2002-05-15
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/30 , C12N9/14 , C12N9/24
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

6. 发明申请

US20090155882A1 Alpha-Amylase Variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US20090155882A1
公开(公告)日：2009-06-18
申请号：US11934136
申请日：2007-11-02
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/28 , C07H21/04 , C12N15/74 , C12N1/20
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

7. 发明授权

US07867746B2 Alpha-amylase variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US07867746B2
公开(公告)日：2011-01-11
申请号：US11934136
申请日：2007-11-02
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/00 , C12N9/28 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

8. 发明申请

US20110076741A1 ALPHA-AMYLASE VARIANTS 有权
公开(公告)号：US20110076741A1
公开(公告)日：2011-03-31
申请号：US12952695
申请日：2010-11-23
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
IPC分类号： C12N9/28 , C12N1/21 , C12N1/19 , C12N15/63 , C07H21/04
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

9. 发明申请

US20110287516A1 Alpha-Amylase Variants 审中-公开
标题翻译： α-淀粉酶变体
公开(公告)号：US20110287516A1
公开(公告)日：2011-11-24
申请号：US13038991
申请日：2011-03-02
申请人： Allan Svendsen , Soeren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
发明人： Allan Svendsen , Soeren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
IPC分类号： C12N9/26
CPC分类号： C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.
摘要翻译：本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。这些变体在高温下（相对于亲本）具有增加的稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

10. 发明授权

US06197565B1 &agr;-Amylase variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US06197565B1
公开(公告)日：2001-03-06
申请号：US09193068
申请日：1998-11-16
申请人： Allan Svendsen , Sóeren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
发明人： Allan Svendsen , Sóeren Kjaerulff , Henrik Bisgaard-Frantzen , Carsten Andersen
IPC分类号： C12N928
CPC分类号： C11D3/38609 , C11D3/386 , C11D3/38618 , C12N9/2417
摘要： The invention relates to a variant of a parent Termamyl-like &agr;-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased.
摘要翻译：本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。这些变体在高温下（相对于亲本）具有增加的稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

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