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1. 发明申请

US20070224688A1 Peptide or protein-capturing surfaces for high throughput MALDI mass spectrometry 审中-公开
公开(公告)号：US20070224688A1
公开(公告)日：2007-09-27
申请号：US11717259
申请日：2007-03-13
申请人： Bernice Feuer , Jinlin Peng , Ma Sha
发明人： Bernice Feuer , Jinlin Peng , Ma Sha
IPC分类号： G01N33/48
CPC分类号： B82Y30/00 , G01N33/54353 , G01N33/545 , Y10T436/24
摘要： Methods and compositions useful for capturing proteins or peptides from a sample mixture and analyzing the captured proteins or peptides via high throughput electrospray ionization (ESI) or matrix-assisted laser desorption/ionization mass spectrometry (HT MALDI MS) are provided. The methods and compositions are useful in large scale, simultaneous analyses of proteins and peptides present in a cell(s), tissue(s), or biological fluid(s).

2. 发明授权

US07214353B2 Peptide or protein-capturing surfaces for high throughput MALDI mass spectrometry 失效
标题翻译：用于高通量MALDI质谱的肽或蛋白质捕获表面
公开(公告)号：US07214353B2
公开(公告)日：2007-05-08
申请号：US10232346
申请日：2002-08-30
申请人： Bernice I. Feuer , Jinlin Peng , Ma Sha
发明人： Bernice I. Feuer , Jinlin Peng , Ma Sha
IPC分类号： B01D11/02
CPC分类号： B82Y30/00 , G01N33/54353 , G01N33/545 , Y10T436/24
摘要： Methods and compositions useful for capturing proteins or peptides from a sample mixture and analyzing the captured proteins or peptides via high throughput electrospray ionization (ESI) or matrix-assisted laser desorption/ionization mass spectrometry (HT MALDI MS) are provided. The methods and compositions are useful in large scale, simultaneous analyses of proteins and peptides present in a cell(s), tissue(s), or biological fluid(s).
摘要翻译：提供了用于从样品混合物中捕获蛋白质或肽的方法和组合物，并通过高通量电喷雾电离（ESI）或基质辅助激光解吸/电离质谱（HT MALDI MS）分析捕获的蛋白质或肽。所述方法和组合物可用于大量，同时分析存在于细胞，组织或生物流体中的蛋白质和肽。

3. 发明授权

US07604984B2 Spatially scanned optical reader system and method for using same 失效
标题翻译：空间扫描光学阅读器系统及其使用方法
公开(公告)号：US07604984B2
公开(公告)日：2009-10-20
申请号：US11027547
申请日：2004-12-29
申请人： Anthony G. Frutos , Jacques Gollier , Jinlin Peng , Garrett A. Piech , Michael B. Webb
发明人： Anthony G. Frutos , Jacques Gollier , Jinlin Peng , Garrett A. Piech , Michael B. Webb
IPC分类号： C12M1/34 , G01N21/00 , G01N21/01 , G01J3/00 , G01J3/28 , G02B6/00 , G02B6/34 , G02B26/08 , G02F1/07
CPC分类号： G01N21/253 , G01N21/554 , G01N21/7743
摘要： An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP). The light source outputs an optical beam which is scanned across a moving biosensor and while this is happening the detector collects the optical beam which is reflected from the biosensor. The computer processes the collected optical beam and records the resulting raw spectral or angle data which is a function of a position (and possibly time) on the biosensor. The processor can then analyze the raw data to create a spatial map of resonant wavelength (peak position) or resonant angle which indicates whether or not a biomolecular binding event occurred on the biosensor. Several other uses of the raw data are also described herein.
摘要翻译：本文描述了一种光学阅读器系统，其使用扫描的光束来询问生物传感器以确定生物分子结合事件是否在生物传感器的表面上发生。在一个实施例中，光学读取器系统包括光源，检测器和处理器（例如，计算机，DSP）。光源输出沿着移动的生物传感器扫描的光束，并且当发生这种情况时，检测器收集从生物传感器反射的光束。计算机处理所收集的光束并记录所生成的原始光谱或角度数据，该数据是生物传感器上的位置（可能是时间）的函数。然后，处理器可以分析原始数据以创建谐振波长（峰值位置）或共振角的空间图，其指示生物传感器上是否发生生物分子结合事件。这里还描述了原始数据的几种其他用途。

4. 发明申请

US20090186776A1 Microcolumn-platform based array for high-throughput analysis 审中-公开
标题翻译：用于高通量分析的基于微柱平台的阵列
公开(公告)号：US20090186776A1
公开(公告)日：2009-07-23
申请号：US12137918
申请日：2008-06-12
申请人： Brian L. Webb , Jinlin Peng , Michael D. Brady , Mircea Despa , Keith A. Horn , Joydeep Lahiri , David M. Root , James B. Stamatoff , Po Ki Yuen
发明人： Brian L. Webb , Jinlin Peng , Michael D. Brady , Mircea Despa , Keith A. Horn , Joydeep Lahiri , David M. Root , James B. Stamatoff , Po Ki Yuen
IPC分类号： C40B30/10 , C40B60/12
CPC分类号： B01L3/50853 , B01J2219/00317 , B01J2219/00504 , B01J2219/00511 , B01J2219/00585 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , C40B60/14 , G01N33/54366 , G01N33/54373 , G01N33/54386 , G01N35/028
摘要： A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.
摘要翻译：提供了用于进行生物或化学分析的装置和方法。该装置包括从支撑板突出的三维微柱阵列。每个微柱具有远离支撑板的相对平面的第一表面。多个不同生物材料的阵列可以附接到第一表面。该装置与现有的微量滴定板结合使用可以提高使用微阵列进行结合测定的高通量能力。

5. 发明申请

US20080160602A1 Microcolumn-based, high-throughput microfluidic device 审中-公开
标题翻译：基于微柱，高通量微流体装置
公开(公告)号：US20080160602A1
公开(公告)日：2008-07-03
申请号：US12002656
申请日：2007-12-18
申请人： Lin He , Jinlin Peng , Youchun Shi , Brian L. Webb , Po Ki Yuen
发明人： Lin He , Jinlin Peng , Youchun Shi , Brian L. Webb , Po Ki Yuen
IPC分类号： C12M1/34
CPC分类号： B01L3/5025 , B01L3/50853 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , G01N33/54366 , G01N35/028 , Y10T436/2575
摘要： A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.
摘要翻译：提供了用于分子生物学，药物研究，基因组分析，组合化学以及可能沉积在各种载体上的分子分析的一般领域的生物测定装置。具体地，本发明包括流体或微流体装置，其将流体性能集成到现有的标准配置的多孔板中，用于以高整体格式进行单一或连续的流体操纵。还提供了这些装置的使用和制造方法。

6. 发明申请

US20080103059A1 Microcolumn-platform based array for high-throughput analysis 审中-公开
公开(公告)号：US20080103059A1
公开(公告)日：2008-05-01
申请号：US12001850
申请日：2007-12-13
申请人： Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
发明人： Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
IPC分类号： C40B30/04
CPC分类号： B01L3/50853 , B01J2219/00317 , B01J2219/00504 , B01J2219/00511 , B01J2219/00585 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , C40B60/14 , G01N33/54366 , G01N33/54373 , G01N33/54386 , G01N35/028
摘要： A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.

7. 发明授权

US07332328B2 Microcolumn-platform based array for high-throughput analysis 失效
标题翻译：用于高通量分析的基于微柱平台的阵列
公开(公告)号：US07332328B2
公开(公告)日：2008-02-19
申请号：US10236120
申请日：2002-09-06
申请人： Brian L. Webb , Jinlin Peng , Michael D. Brady , Mircea Despa , Keith A. Horn , Joydeep Lahiri , David M. Root , James B. Stamatoff , Po Ki Yuen
发明人： Brian L. Webb , Jinlin Peng , Michael D. Brady , Mircea Despa , Keith A. Horn , Joydeep Lahiri , David M. Root , James B. Stamatoff , Po Ki Yuen
IPC分类号： C12M1/34
CPC分类号： B01L3/50853 , B01J2219/00317 , B01J2219/00504 , B01J2219/00511 , B01J2219/00585 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , C40B60/14 , G01N33/54366 , G01N33/54373 , G01N33/54386 , G01N35/028
摘要： A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.
摘要翻译：提供了用于进行生物或化学分析的装置和方法。该装置包括从支撑板突出的三维微柱阵列。每个微柱具有远离支撑板的相对平面的第一表面。多个不同生物材料的阵列可以附接到第一表面。该装置与现有的微量滴定板结合使用可以提高使用微阵列进行结合测定的高通量能力。

8. 发明申请

US20070238197A9 Fluorescent ligands for GPCR arrays 审中-公开
标题翻译： GPCR阵列的荧光配体
公开(公告)号：US20070238197A9
公开(公告)日：2007-10-11
申请号：US11363400
申请日：2006-02-27
申请人： Ye Fang , Yulong Hong , Jinlin Peng
发明人： Ye Fang , Yulong Hong , Jinlin Peng
IPC分类号： G01N33/543
CPC分类号： G01N33/582 , C07K7/083 , C07K14/63 , G01N33/6845 , G01N33/74 , G01N2333/63 , G01N2333/726
摘要： Microarrays employing a fluorescent ligand including a material having a binding affinity in the range of about 0.01 to about 25 nM, or about 0.1 to about 10 nM; a specificity to its cognate receptor in the range of about 50 to about 99%, or about 65 to about 99%; a cross-activity to other receptors of 0 to about 20%, or 0 to about 10%; a net charge per ligand of about −3 to about +5, or more preferably, about −2 to about +2 or most preferably for small compound ligands about −1 to about +2. The ligand may also have a hydrophobicity in the range of about 3 to about 55 minutes eluting time (as measured under specified eluting conditions). In some embodiments, the ligand includes fluorescently labeled motilin 1-16 labeled with Bodipy-TMR, rhodamine or Cy5-. Other embodiments include fluorescently labeled Cy5-naltrexone, Cy5-neurotensin 2-13, N-terminal labeled neurotensin 2-13 or lys-labeled labeled neurotensin 2-13.
摘要翻译：使用荧光配体的微阵列，其包括具有约0.01至约25nM或约0.1至约10nM范围内的结合亲和力的材料; 在约50至约99％，或约65至约99％的范围内对其同源受体的特异性; 与其他受体的交叉活性为0至约20％，或0至约10％; 每个配体的净电荷为约-3至约+5，或更优选约-2至约+2，或最优选约为-1至约+2的小化合物配体。配体还可以具有约3至约55分钟洗脱时间（在规定的洗脱条件下测量）的疏水性。在一些实施方案中，配体包括用Bodipy-TMR，罗丹明或Cy5-标记的荧光标记的胃动素1-16。其他实施方案包括荧光标记的Cy5-纳曲酮，Cy5-神经丝氨酸2-13，N-末端标记的神经丝氨酸蛋白酶2-13或lys-标记的标记的神经丝氨酸素2-13。

9. 发明申请

US20120244568A1 LABEL-FREE RIGID CELL ASSAY METHOD 审中-公开
标题翻译：无标签电池测试方法
公开(公告)号：US20120244568A1
公开(公告)日：2012-09-27
申请号：US13357969
申请日：2012-01-25
申请人： Fang Lai , Guangshan Li , Anping Liu , Jinlin Peng
发明人： Fang Lai , Guangshan Li , Anping Liu , Jinlin Peng
IPC分类号： C12Q1/02
CPC分类号： G01N21/553
摘要： A label-free cell assay method including: culturing rigid cells in a buffer, the rigid cells having a diameter of about 3 to about 7 micrometers; depositing the rigid cells on the surface of a sensor system; and detecting the rigid cells with the sensor when exposed to two or more wavelengths of light having two or more penetration depths, as defined herein. Also disclosed are assay methods for contacting the rigid cells with a substance and determining the response of the contacted rigid cells to the substance, such as a drug candidate or modulator compound, as defined herein.
摘要翻译：无标记细胞测定方法，包括：在缓冲液中培养刚性细胞，刚性细胞具有约3至约7微米的直径; 将刚性电池沉积在传感器系统的表面上; 并且当暴露于具有两个或更多个穿透深度的两个或更多个波长的光，如本文所定义时，用传感器检测刚性单元。还公开了用于使刚性细胞与物质接触并测定接触的刚性细胞对如本文所定义的药物候选物或调节剂化合物的物质的反应的测定方法。

10. 发明申请

US20110043828A1 OPTICAL READER SYSTEM AND METHOD FOR MONITORING AND CORRECTING LATERAL AND ANGULAR MISALIGNMENTS OF LABEL INDEPENDENT BIOSENSORS 审中-公开
标题翻译：用于监测和校正标签独立生物传感器的横向和角度误差的光学读取器系统和方法
公开(公告)号：US20110043828A1
公开(公告)日：2011-02-24
申请号：US12938740
申请日：2010-11-03
申请人： Anthony G. Frutos , Jacques Gollier , Jinlin Peng , Garrett A. Piech , Michael B. Webb
发明人： Anthony G. Frutos , Jacques Gollier , Jinlin Peng , Garrett A. Piech , Michael B. Webb
IPC分类号： G01N21/01 , G01B11/14
CPC分类号： G01N21/553 , G01N21/7743
摘要： An optical reader system and method are described herein that can detect a lateral and/or angular misalignment of one or more biosensors so that the biosensors can be properly re-located after being removed from and then reinserted into the optical reader system. In one embodiment, the biosensors are incorporated within the wells of a microplate.
摘要翻译：本文描述了可以检测一个或多个生物传感器的侧向和/或角度偏移的光学读取器系统和方法，使得生物传感器可以在从光学读取器系统中移除并重新插入到光学读取器系统之后被适当地重新定位。在一个实施方案中，将生物传感器并入微板的孔内。

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