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1. 发明申请

US20170226523A1 METHODS FOR SETARIA VIRIDIS TRANSFORMATION 审中-公开
公开(公告)号：US20170226523A1
公开(公告)日：2017-08-10
申请号：US15501916
申请日：2015-08-06
申请人： Benson Hill Biosystems, Inc.
发明人： Xiuhua Chen , Xingrong Wu
IPC分类号： C12N15/82 , A01H4/00
CPC分类号： C12N15/8205 , A01H4/008 , C12N15/8203
摘要： This invention relates to methods for the transformation of Setaria species such as Setaria viridis and transformed plants produced according to the method. Specifically, this invention relates to direct transformation of callus derived from mature embryos using Agrobacterium-mediated transformation, and plants regenerated from the transformed callus tissue. The methods comprise utilizing Setaria mature embryos as the source of plant material for callus induction; induced calli can be infected by Agrobacterium hosting an appropriate vector. Transgenic plants are regenerated from transgenic calli grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells. These methods provide for significantly increased plant transformation efficiency with minimal ratio of escapes.

2. 发明申请

US20160090602A1 METHODS FOR PLANT TRANSFORMATION 审中-公开
标题翻译：植物转化方法
公开(公告)号：US20160090602A1
公开(公告)日：2016-03-31
申请号：US14863532
申请日：2015-09-24
申请人： BENSON HILL BIOSYSTEMS, INC.
发明人： Xingrong Wu
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： This invention relates to methods for the transformation of Brachypodium sp. and transformed plants produced according to the method. Specifically, this invention relates to direct transformation of callus derived from immature embryos using Agrobacterium-mediated transformation, and plants regenerated from the transformed callus tissue.The methods comprise utilizing Brachypodium sp. immature embryos as the source of plant material for callus induction; induced calli can be infected by Agrobacterium hosting an appropriate binary vector. Transgenic plants are regenerated from transgenic calli grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells. These methods provide for significantly increased plant transformation efficiency with minimal ratio of escapes.
摘要翻译：本发明涉及一种用于转化Brachypodium sp。和根据该方法生产的转化植物。具体地，本发明涉及使用农杆菌介导的转化从未成熟胚产生的愈伤组织的直接转化，以及从转化的愈伤组织再生的植物。该方法包括使用Brachypodium sp。未成熟胚作为愈伤组织诱导的植物材料的来源; 诱导的愈伤组织可以被承载适当的二元载体的农杆菌感染。转基因植物从转基因生长条件下生长的转基因愈伤组织再生，同时基本上抑制非转化细胞的生长。这些方法提供了显着增加的植物转化效率，并以最小的逃逸比例。

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