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1. 发明授权

US06200793B1 Cold-active protease CP70 失效
标题翻译：冷活性蛋白酶CP70
公开(公告)号：US06200793B1
公开(公告)日：2001-03-13
申请号：US09117245
申请日：1998-07-27
申请人： A. K. M. Quamrul Hasan , Eiichi Tamiya
发明人： A. K. M. Quamrul Hasan , Eiichi Tamiya
IPC分类号： C12N952
CPC分类号： A23J3/341 , C12N9/52 , Y10S435/85
摘要： A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them, and the substrate specificity decreases in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable in the range of pH 6.5 to pH 10.0 at 30° C. for 1 hour; (d) optimal temperature: about 40° C.; (e) temperature stability: at pH 7 for 1 hour, the protease is active at a temperature up to 30° C., but it is inactivated at 40° C. as much as about 40% and completely inactivated at 50° C. in about 10 minutes; (f) enzyme activity: the protease has 50% or more of its maximum activity at about 20° C.; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.
摘要翻译：这里公开了一种嗜热蛋白酶，其具有以下物理化学性质：（a）比活性和底物特异性：蛋白酶作用于酪蛋白，明胶，血红蛋白和白蛋白以特异性分解它们，底物特异性按酪蛋白，明胶的顺序降低，血红蛋白和白蛋白; （b）最佳pH：8.0; （c）pH稳定性：蛋白酶在30℃下在pH 6.5至pH 10.0范围内稳定1小时; （d）最佳温度：约40℃。（e）温度稳定性：在pH7下1小时，蛋白酶在高达30℃的温度下是活性的，但在40℃下灭活达40％左右，并在50℃完全失活。约10分钟; （f）酶活性：蛋白酶在约20℃下具有50％以上的最大活性; （g）酶的活性中心是丝氨酸; 和（h）通过SDS-PAGE测量，蛋白酶的分子量为约70kDa。

2. 发明授权

US08445193B2 Microwell array chip for detecting antigen-specific lymphocytes, method of detecting and method of manufacturing antigen-specific lymphocytes, and method of cloning antigen-specific lymphocyte antigen receptor genes 有权
标题翻译：用于检测抗原特异性淋巴细胞的微孔阵列芯片，检测方法和抗原特异性淋巴细胞的制造方法以及克隆抗原特异性淋巴细胞抗原受体基因的方法
公开(公告)号：US08445193B2
公开(公告)日：2013-05-21
申请号：US12337481
申请日：2008-12-17
申请人： Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
发明人： Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
IPC分类号： G01N33/53 , C12N5/06 , C12N5/08 , C12N5/00
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.
摘要翻译：微孔阵列芯片具有多个微孔，并用于在每个微孔中含有单个淋巴细胞标本，并以单个单位检测抗原特异性淋巴细胞; 其中所述微孔阵列芯片的形状和尺寸在每个微孔中仅包含一个淋巴细胞。一种检测抗原特异性淋巴细胞的方法，包括以下步骤：向上述微孔阵列芯片中的每个微孔加入抗原，刺激淋巴细胞标本，以及检测与抗原反应的淋巴细胞标本。

3. 发明申请

US20090159458A1 Method for Determination of Test Substance 审中-公开
标题翻译：测定物质的测定方法
公开(公告)号：US20090159458A1
公开(公告)日：2009-06-25
申请号：US12226060
申请日：2007-03-29
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yuhi , Kagan Kerman , Koutarou Idegami , Miyuki Chikae
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yuhi , Kagan Kerman , Koutarou Idegami , Miyuki Chikae
IPC分类号： G01N27/416 , G01N33/543
CPC分类号： G01N33/5438
摘要： [Problems] A highly sensitive and accurate determination of a test substance is achieved without the need of complicated procedures.[Means for solving the Problems] The presence or absence or the concentration of a test substance 3 can be determined by gathering around the surface of a working electrode 1 metal microparticles 5 in an amount corresponding to the amount of the test substance 3 contained in a test solution, oxidizing the metal microparticles 5 electrochemically, measuring the value of a current produced by electrochemically reducing the oxidized metal, and determining the presence or absence or the concentration of the substance 3 based on the current value. It is preferred that the electrochemical oxidation of the metal microparticles 5 be conducted while maintaining the potential of the working electrode 1 at a value equal to a potential employed for the electrochemical oxidization of the metal microparticles 5.
摘要翻译： [问题]在不需要复杂程序的情况下实现对测试物质的高度灵敏和准确的测定。 [解决问题的方法]测试物质3的存在或不存在或浓度可以通过将工作电极1的金属微粒5的表面聚集在与金属微粒5中包含的测试物质3的量相当的量测试溶液，电化学氧化金属微粒5，测量通过电化学还原氧化金属产生的电流的值，并根据当前值确定物质3的存在或不存在或浓度。优选的是，在将工作电极1的电位保持在与金属微粒5的电化学氧化的电位相等的值的情况下，进行金属微粒5的电化学氧化。

4. 发明申请

US20060078946A1 Method for manufacturing antigen-specific antibody-producing hybridomas employing a single antigen-specific B lymphocyte and method for manufacturing monoclonal antibody 审中-公开
标题翻译：使用单一抗原特异性B淋巴细胞制造抗原特异性抗体产生杂交瘤的方法和用于制造单克隆抗体的方法
公开(公告)号：US20060078946A1
公开(公告)日：2006-04-13
申请号：US11236553
申请日：2005-09-28
申请人： Atsushi Muraguchi , Hiroyuki Kishi , Kihachiro Tohbo , Minoru Ueno , Hiroyoshi Nakazato , Eiichi Tamiya , Masayasu Suzuki
发明人： Atsushi Muraguchi , Hiroyuki Kishi , Kihachiro Tohbo , Minoru Ueno , Hiroyoshi Nakazato , Eiichi Tamiya , Masayasu Suzuki
IPC分类号： G01N33/567 , C12P21/04 , G01N33/53
CPC分类号： C07K16/00 , C12N5/163
摘要： A method for producing a monoclonal antibody and antigen-specific antibody-producing hybridomas using a single antigen-specific B lymphocyte is provided. The method for producing antigen-specific antibody-producing hybridomas comprises selecting a single B lymphocyte reacting specifically with a certain antigen, an “antigen-specific B lymphocyte”, culturing the antigen-specific B lymphocyte selected, and fusing the cultured antigen-specific B lymphocyte with myeloma cells to obtain the hybridomas. The monoclonal antibody is produced with thus obtained hybridomas.
摘要翻译：提供了使用单一抗原特异性B淋巴细胞产生单克隆抗体和产生抗原特异性抗体的杂交瘤的方法。产生产生抗原特异性抗体的杂交瘤的方法包括选择与特定抗原特异性反应的单一B淋巴细胞，“抗原特异性B淋巴细胞”，培养所选择的抗原特异性B淋巴细胞，并将培养的抗原特异性B 淋巴细胞与骨髓瘤细胞以获得杂交瘤。用如此获得的杂交瘤产生单克隆抗体。

5. 发明申请

US20090130384A1 Chip Provided with film Having Hole Pattern with the Use of Thermoresponsive Polymer and Method of Producing the Same 审中-公开
标题翻译：带有膜的芯片具有使用热聚合物的孔图案及其制造方法
公开(公告)号：US20090130384A1
公开(公告)日：2009-05-21
申请号：US11992594
申请日：2006-09-27
申请人： Eiichi Tamiya , Yoshiyuki Yokoyama , Satoshi Fujiki , Katsumi Tanino , Atsushi Muraguchi , Horoyuki Kishi , Yoshiharu Tokimitsu , Shohei Yamamura
发明人： Eiichi Tamiya , Yoshiyuki Yokoyama , Satoshi Fujiki , Katsumi Tanino , Atsushi Muraguchi , Horoyuki Kishi , Yoshiharu Tokimitsu , Shohei Yamamura
IPC分类号： B32B3/10 , C12N11/04 , B05D3/00 , G03F7/20
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2535/10 , C12N2537/10 , C12N2539/10 , Y10T428/24273 , Y10T436/25 , Y10T436/25375
摘要： [Problems] To provide a novel chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation.[Means for Solving Problems] A chip comprising a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing a chip which comprises a crosslinked product of a temperature-responsive polymer as a constituting member and is provided with a film having a hole pattern on the surface of the baseboard. This method comprises applying a composition containing a crosslinkable temperature-responsive polymer, a composition containing a crosslinkable temperature-responsive polymer and a crosslinking agent or a composition containing a temperature-responsive polymer and a crosslinking agent on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.
摘要翻译： [问题]提供一种用于处理细胞等的新颖的芯片，其具有机构和结构，其中孔图案的尺寸任意改变，使得细胞可以容易地在散射或收集细胞中从孔中移出和离开但在洗涤或抗原刺激期间几乎不能从孔中脱出。解决问题的手段一种芯片，其包含作为构成部件的温度敏感性聚合物的交联体，并且在基板的表面设置有具有孔图案的膜。一种制造芯片的方法，其包括作为构成部件的温度敏感性聚合物的交联产物，并且在基板的表面上设置有具有孔图案的膜。该方法包括在基板的表面上涂布含有交联性温度响应性聚合物的组合物，含有交联性温度敏感性聚合物和交联剂的组合物或含有温度敏感性聚合物和交联剂的组合物，从而形成使涂膜交联，由此形成如上所述的交联产物，然后在交联产物的涂膜上形成孔图案。

6. 发明授权

US08110406B2 Analytical method and analytical apparatus 失效
标题翻译：分析方法和分析仪器
公开(公告)号：US08110406B2
公开(公告)日：2012-02-07
申请号：US12085466
申请日：2006-11-27
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , Y10S435/97
摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.
摘要翻译： [问题]减少例如所用的捕获抗体的量而不降低检测灵敏度。同时，即使在只有少量的标记抗体被累积的情况下，也可以在确定区域中实现强烈的显色或发光。降低夹心法的检测限。在比赛方式中扩大动态范围。用于解决问题的手段通过使用被检物质的免疫学分析方法分析检测物质的方法，具有测定区域的载体，其中选自能够特异性结合测试物质的捕获抗体中的一个和已经固定了能够特异性结合测试物质的捕获抗原，并且能够特异性结合测试物质的标记抗体，其中具有敏化作用的标签已被固定在载体的确定区域上。

7. 发明申请

US20090047746A1 Analytical Method and Analytical Apparatus 失效
标题翻译：分析方法和分析仪器
公开(公告)号：US20090047746A1
公开(公告)日：2009-02-19
申请号：US12085466
申请日：2006-11-27
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
IPC分类号： G01N33/543 , G01N21/00
CPC分类号： G01N33/54393 , Y10S435/97
摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.
摘要翻译： [问题]减少例如所用的捕获抗体的量而不降低检测灵敏度。同时，即使在只有少量的标记抗体被累积的情况下，也可以在确定区域中实现强烈的显色或发光。降低夹心法的检测限。在比赛方式中扩大动态范围。用于解决问题的手段通过使用被检物质的免疫学分析方法分析检测物质的方法，具有测定区域的载体，其中选自能够特异性结合测试物质的捕获抗体中的一个和已经固定了能够特异性结合测试物质的捕获抗原，并且能够特异性结合测试物质的标记抗体，其中具有敏化作用的标签已被固定在载体的确定区域上。

8. 发明申请

US20060134704A1 Microwell array chip for detecting antigen-specific lymphocytes, method of detecting and method of manufacturing antigen-specific lymphocytes, and method of cloning antigen-specific lymphocyte antigen receptor genes 审中-公开
公开(公告)号：US20060134704A1
公开(公告)日：2006-06-22
申请号：US10534800
申请日：2003-09-30
申请人： Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
发明人： Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
IPC分类号： G01N33/567 , C12M1/34 , G01N33/53
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.

9. 发明申请

US20140220668A1 MICROFLUIDIC DEVICE 审中-公开
标题翻译：微流体装置
公开(公告)号：US20140220668A1
公开(公告)日：2014-08-07
申请号：US14240036
申请日：2012-08-21
申请人： Hiroaki Tachibana , Koji Tsuji , Takashi Saijo , Eiichi Tamiya , Masato Saito
发明人： Hiroaki Tachibana , Koji Tsuji , Takashi Saijo , Eiichi Tamiya , Masato Saito
IPC分类号： B01L7/00 , C12Q1/68
CPC分类号： B01L7/52 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00831 , B01J2219/00853 , B01J2219/00869 , B01J2219/00873 , B01J2219/00912 , B01J2219/00957 , B01J2219/00961 , B01L3/502707 , B01L7/525 , B01L2300/0645 , B01L2300/0816 , B01L2300/0883 , B01L2300/0887 , B01L2300/0893 , B01L2300/1827 , C12M23/16 , C12Q1/6844 , G01N27/44708 , G01N27/44791
摘要： To provide a microfluidic device which does not apply a meandering reaction channel and thus can be reduced in size, the microfluidic device comprising a reaction channel is characterized in that a plurality of thermal cycle regions which respectively comprise at least two thermal regions with different temperatures are repeatedly provided and the reaction channel passes through the plurality of thermal cycle regions.
摘要翻译：为了提供不应用曲折反应通道并因此可以减小尺寸的微流体装置，包括反应通道的微流体装置的特征在于分别包括至少两个具有不同温度的热区域的多个热循环区域是反复提供并且反应通道穿过多个热循环区域。

10. 发明授权

US07875825B2 Plasma generating equipment 有权
标题翻译：等离子发生设备
公开(公告)号：US07875825B2
公开(公告)日：2011-01-25
申请号：US10593771
申请日：2005-03-25
申请人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
发明人： Yuzuru Takamura , Akiko Iiduka , Eiichi Tamiya
IPC分类号： B23K9/02 , G01J3/30
CPC分类号： G01N21/68 , G01N21/67 , G01N21/69
摘要： A method for generating plasma and a method for elemental analysis, each comprising the steps of providing a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; filling the flow channel and the narrow portion with a conductive liquid, and thereafter applying an electric field to the narrow portion, to conduct the electric field through the narrow portion, thereby generating plasma at the narrow portion. An apparatus for generating plasma, the apparatus for generating plasma comprising a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; and a means of applying an electric field to the narrow portion to conduct the electric field through the narrow portion; and an apparatus for emission spectroscopic analysis comprising the apparatus for generating plasma as defined above.
摘要翻译：一种用于产生等离子体的方法和元素分析方法，每个方法包括以下步骤：在由绝缘材料制成的流动通道中提供窄部分，所述窄部分的横截面积明显小于所述绝缘材料的横截面面积流通道用导电液体填充流路和狭窄部分，然后向狭窄部分施加电场，使电场通过狭窄部分，从而在狭窄部分产生等离子体。一种用于产生等离子体的装置，所述用于产生等离子体的装置包括由绝缘材料制成的流动通道中的窄部分，所述窄部分具有明显小于所述流动通道的横截面积的横截面面积; 以及向狭窄部分施加电场以通过所述狭窄部分传导电场的装置; 以及用于发射光谱分析的装置，其包括如上所述产生等离子体的装置。

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