专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10822612B2 Chimeric proteins which enhance the activity of DNA binding domains (DBD) and transcription factors in plants 有权
公开(公告)号：US10822612B2
公开(公告)日：2020-11-03
申请号：US15537047
申请日：2015-12-16
申请人： CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET) , UNIVERSIDAD NACIONAL DE ROSARIO
发明人： Carla Schommer , Javier Palatnik , Juan Manuel Debernardi , Ramiro Esteban Rodriguez Virasoro
IPC分类号： C12N15/82 , C07K14/415
摘要： The present invention relates to a method of enhancing the activity of a transcription factor in a plant comprising expressing in said plant a chimeric protein comprising a GIF moiety comprising a GIF domain or fragment thereof, and a DNA binding domain (DBD) moiety. The chimeric proteins comprise a GIF moiety comprising a GIF domain or fragment thereof, or a polypeptide with an amino acid sequence having at least 85%, preferably at least 90%, even more preferably at least 95% sequence identity to the amino acid sequence of a GIF domain or fragment thereof; and a DBD moiety comprising the DBD or plant transcription factor whose activity enhancement is intended, or a binding protein which is capable of forming a tertiary complex with the DBD or transcription factor whose activity modification and/or enhancement is intended. The invention also relates to polynucleotides and polypeptides useful in the method of the invention.

2. 发明申请

US20170362601A1 CHIMERIC PROTEINS WHICH ENHANCE THE ACTIVITY OF DNA BINDING DOMAINS (DBD) AND TRANSCRIPTION FACTORS IN PLANTS 审中-公开
公开(公告)号：US20170362601A1
公开(公告)日：2017-12-21
申请号：US15537047
申请日：2015-12-16
申请人： Carla SCHOMMER , CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET) , UNIVERSIDAD NACIONAL DE ROSARIO
发明人： Carla SCHOMMER , Javier PALATNIK , Juan Manuel DEBERNARDI , Ramiro Esteban RODRIGUEZ VIRASORO
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8216 , C07K14/415 , C07K2319/00 , C07K2319/80 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method of enhancing the activity of a transcription factor in a plant comprising expressing in said plant a chimeric protein comprising a GIF moiety comprising a GIF domain or fragment thereof, and a DNA binding domain (DBD) moiety. The chimeric proteins comprise a GIF moiety comprising a GIF domain or fragment thereof, or a polypeptide with an amino acid sequence having at least 85%, preferably at least 90%, even more preferably at least 95% sequence identity to the amino acid sequence of a GIF domain or fragment thereof; and a DBD moiety comprising the DBD or plant transcription factor whose activity enhancement is intended, or a binding protein which is capable of forming a tertiary complex with the DBD or transcription factor whose activity modification and/or enhancement is intended. The invention also relates to polynucleotides and polypeptides useful in the method of the invention.

3. 发明授权

US09890388B2 GRF3 mutants, methods and plants 有权
公开(公告)号：US09890388B2
公开(公告)日：2018-02-13
申请号：US14370361
申请日：2013-01-04
申请人： Universidad Nacional de Rosario , Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET)
发明人： Javier Palatnik , Ramiro Rodriguez , Martin Mecchia , Juan Manuel Debernardi
IPC分类号： A01H5/00 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8202 , C12N15/8218 , C12N15/8266 , C12N15/8273 , Y02A40/146
摘要： The present disclosure provides a novel modified gene, rGRF3, or an ortholog thereof, which is shown to be decoupled from control by miR396, particularly in the presence of over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof. When present in a plant, the rGRF3 results in a phenotype of increased productivity (e.g. increased yield, increased biomass, increased stress resistance, increased seed production, increased seed yield, increased root growth, increased root elongation speed, delayed leaf senescence or increased drought tolerance and combinations thereof).

4. 发明申请

US20210202032A1 METHOD OF TAGGING NUCLEIC ACID SEQUENCES, COMPOSITION AND USE THEREOF 有权
公开(公告)号：US20210202032A1
公开(公告)日：2021-07-01
申请号：US16757655
申请日：2018-10-19
申请人： CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET) , UNIVERSIDAD NACIONAL DE ROSARIO (UNR)
发明人： Flavia Jorgelina KRSTICEVIC , Joaquín EZPELETA , Pilar Estela BULACIO , Elizabeth TAPIA
IPC分类号： G16B25/10 , G16B30/10
摘要： A first aspect of the present invention relates to a method of tagging a nucleic acid molecule with a predetermined ID number, the method comprising: (a) attaching to said nucleic acid molecule a nucleic acid tag to form a tagged nucleic acid molecule, wherein said nucleic acid tag comprises one or more nucleic acid tag sub-units each consisting of groups of at least two nucleotides, wherein said nucleic acid tag is attributed said ID number by performing the following steps: (i) converting each nucleotide in said nucleic acid tag sub-units into a number ranging from 0 to 3, thereby creating numerical tag sub-units, wherein the distribution and content of the nucleotides in the nucleic acid tag sub-units has been configured to allow a finite number of numerical tag sub-units, (ii) attributing to each of said numerical tag sub-units a predetermined numerical tag element, thereby creating a numerical tag, (iii) linearly decoding said numerical tag, thereby creating said ID number. A second aspect of the present invention relates to a method for multiplex sequencing and/or demultiplexing, the method comprising the steps of: (a) multiplexing amplification of tagged nucleic acid molecules obtained according to the method of the first aspect of the present invention to generate a plurality of said tagged nucleic acid molecules, (b) pooling and parallel sequencing said plurality of tagged nucleic acid molecules, thereby generating a plurality of sequence reads, and (c) demultiplexing said plurality of sequence reads, wherein each of said sequence reads is attributed to a sample. A third aspect of the present invention is directed to a tagged nucleic acid molecule construed according to the method of the first aspect of the present invention and its use in a method for multiplex sequencing and/or demultiplexing. A fourth aspect of the present invention relates to an apparatus configured for multiplex sequencing demultiplexing, the apparatus comprising: tools for designing nucleic acid tags according to the method described in the first aspect of the present invention, tools for pooling and multiplexing a plurality of tagged nucleic acid sequences, a sequence demultiplexer, additional tools for data reproduction and post-sequencing analysis. A fifth aspect of the present invention, there is provided a method of tagging a nucleic acid molecule with a predetermined ID number, the method comprising: (a) attaching to said nucleic acid molecule a nucleic acid tag to form a tagged nucleic acid molecule, wherein said nucleic acid tag comprises one or more nucleic acid tag sub-units each consisting of groups of at least two nucleotides, wherein said nucleic acid tag is obtained from said ID number by performing the following steps: (i) linearly encoding said ID number, thereby creating a numerical tag, wherein the numerical tag comprises a plurality of numerical tag elements; (ii) attributing to each of said numerical tag elements a numerical tag sub unit, wherein the numerical tag sub-unit comprises a plurality of numbers ranging from 0 to 3; (iii) attributing to each of said numerical tag sub-units a nucleic acid tag sub-unit, thereby creating said nucleic acid tag, wherein the distribution and content of the nucleotides in the nucleic acid tag sub units has been configured to allow a finite number of numerical tag sub-units.

5. 发明申请

US20150033413A1 GRF3 Mutants, Methods and Plants 有权
标题翻译： GRF3突变体，方法和植物
公开(公告)号：US20150033413A1
公开(公告)日：2015-01-29
申请号：US14370361
申请日：2013-01-04
申请人： Universidad Nacional de Rosario , Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET)
发明人： Javier Palatnik , Ramiro Rodriguez , Martin Mecchia , Juan Manuel Debernandi
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8202 , C12N15/8218 , C12N15/8266 , C12N15/8273 , Y02A40/146
摘要： The present disclosure provides a novel modified gene, rGRF3, or an orthologue thereof, which is shown to be decoupled from control by miR396, particularly in the presence of over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof. When present in a plant, the rGRF3 results in a phenotype of increased productivity (e.g. increased yield, increased biomass, increased stress resistance, increased seed production, increased seed yield, increased root growth, increased root elongation speed, delayed leaf senescence or increased drought tolerance and combinations thereof).
摘要翻译：本公开提供了一种新的修饰基因rGRF3或其直向同源物，其显示与miR396的对照分离，特别是在存在至少一种GIF基因如GIF1，AtGIF2，AtGIF的过表达的情况下 3，Os11g40100，Os12g31350，Os03g52320或其组合。当存在于植物中时，rGRF3产生增加生产力的表型（例如增加的产量，增加的生物量，增加的胁迫抗性，增加的种子产量，增加的种子产量，增加的根生长，增加的根伸长速度，延迟叶衰老或增加的干旱公差及其组合）。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式