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1. 发明申请

US20120255051A1 HERBICIDE TOLERANT PLANTS 审中-公开
标题翻译：除草剂耐受植物
公开(公告)号：US20120255051A1
公开(公告)日：2012-10-04
申请号：US13514151
申请日：2010-12-06
申请人： Rene Ruiter , Timothy Golds
发明人： Rene Ruiter , Timothy Golds
IPC分类号： C12N15/54 , A01H5/10 , A01P13/00 , A01H1/02 , A01H1/06 , A01N47/38 , A01H5/00 , C12N5/10
CPC分类号： A01H5/10 , C12N9/88 , C12N15/8278
摘要： The present invention relates to Brassica plants comprising full knockout AHAS alleles and to brassica plant comprising a combination of full knockout AHAS alleles and AHAS alleles encoding herbicide tolerant AHAS proteins, nucleic acid sequences representing full knockout AHAS alleles, as well as methods for generating and identifying said plants and alleles, which can be used to obtain herbicide tolerant plants.
摘要翻译：本发明涉及包含完全敲除AHAS等位基因的芸苔属植物，以及包含完全敲除的AHAS等位基因和编码除草剂耐药AHAS蛋白质的AHAS等位基因的组合的芸苔属植物，代表完全敲除的AHAS等位基因的核酸序列，以及用于产生和鉴定所述植物和等位基因可用于获得耐除草剂植物。

2. 发明申请

US20060117400A1 Plastid transformation using modular vectors 有权
标题翻译：使用模块化矢量的质体变换
公开(公告)号：US20060117400A1
公开(公告)日：2006-06-01
申请号：US10523474
申请日：2003-08-01
申请人： Stefan Herz , Hans Koop , Timothy Golds , Christian Eibl
发明人： Stefan Herz , Hans Koop , Timothy Golds , Christian Eibl
IPC分类号： A01H1/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8214
摘要： A process of generating transgenic plants or plant cells transformed on their plastome, comprising (a) introducing into plant plastids a first DNA molecule and a second DNA molecule, wherein said first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and said second DNA molecule contains a second region homologous to a region of the plastome for directing plastome integration and a second sequence of interest, whereby a sequence segment of said first sequence of interest is homologous to a sequence segment of said second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby said integration sequence contains at least a portion of said first and at least a portion of said second sequence of interest as a continuous sequence.
摘要翻译：一种产生在其塑料体上转化的转基因植物或植物细胞的方法，包括（a）向植物质体中引入第一DNA分子和第二DNA分子，其中所述第一DNA分子包含与所述塑性体的区域同源的第一区域，用于引导 plastome整合和第一个兴趣序列，并且所述第二DNA分子含有与所述plastome区域同源的第二区域，用于引导Plastome整合和第二感兴趣的序列，其中所述第一感兴趣序列的序列片段与所述第二感兴趣序列的序列片段，以及选择具有稳定整合在所述塑性体中的整合序列的转化体，由此所述整合序列包含所述第一和至少一部分所述第二感兴趣序列的连续序列的至少一部分。

3. 发明申请

US20050015829A1 Gene expression in plastids based on replicating vectors 失效
标题翻译：基于复制载体的质体中的基因表达
公开(公告)号：US20050015829A1
公开(公告)日：2005-01-20
申请号：US10482549
申请日：2002-04-30
申请人： Hans-Ulrich Koop , Stefan Muhlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy Golds
发明人： Hans-Ulrich Koop , Stefan Muhlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy Golds
IPC分类号： A01H1/00 , A01H5/00 , C12N5/10 , C12N15/09 , C12N15/82 , C12N15/63 , C12N15/87
CPC分类号： C12N15/8212 , C12N15/821 , C12N15/8214 , C12N15/8257
摘要： A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.
摘要翻译：一种产生转化的植物或植物细胞的方法，所述转基因植物或植物细胞在其塑料体上转化并且没有选择标记，包括以下步骤：（a）用DNA转化植物或植物细胞的质体，所述DNA包括：（i）赋予复制的核苷酸序列所述DNA在植物细胞中，（ii）至少一种感兴趣的序列，（iii）侧翼于所述至少一个感兴趣序列侧翼的序列，所述至少一个感兴趣序列用于将所述至少一个感兴趣的序列稳定整合到质体基因组中，以及（iv ）在所述感兴趣的序列侧翼的所述序列之外的选择标记; （b）允许在存在选择压力的情况下将所述至少一个感兴趣序列整合到塑料瓶中; （c）通过释放选择压力允许所述选择标记序列的丢失; 和（d）回收在其塑料上遗传转化并且没有所述选择标记的细胞和/或植物。

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