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1. 发明申请

US20070218500A1 Method and Apparatus for Detection of Live Bacterium Within Test Subject Through Specifically labeling Thereof 审中-公开
标题翻译：通过特异性标记检测受试者中活细菌的方法和装置
公开(公告)号：US20070218500A1
公开(公告)日：2007-09-20
申请号：US11587846
申请日：2005-03-03
申请人： Tohru Mikoshiba , Tetsuro Sasaki , Takaharu Enjoji
发明人： Tohru Mikoshiba , Tetsuro Sasaki , Takaharu Enjoji
IPC分类号： G01N33/53
CPC分类号： G01N33/56911 , B01J20/286 , B01J20/3242 , B01J2220/52 , B01J2220/54 , B01J2220/58 , G01N21/6428 , G01N21/78 , G01N33/58
摘要： A method and apparatus for detection whereby live bacteria among microbes as an antigen can be detected rapidly in a short period of time through specifically labeling of live bacteria within a test subject antigen and whereby testing assurance can be ensured. The method and apparatus are characterized in that labeled antigen (14) is formed by action, on a test subject antigen such as Escherichia coli, of labeled substance (13) zymolyzable by live bacteria (target bacteria (12)) within the test subject antigen, and the resultant labeled antigen (14) is trapped on an immobilization phase having, immobilized thereon, a specific binding antibody capable of specifically binding to the test subject antigen.
摘要翻译：一种用于检测的方法和装置，其中通过特异性标记测试受试者抗原内的活细菌，可以在短时间内快速检测作为抗原的微生物中的活细菌，并且由此可以确保测试保证。所述方法和装置的特征在于，通过作用于被检对象抗原（例如大肠杆菌）的受试对象抗原中的活细菌（靶细菌（12））可酶解的标记物质（13）的作用形成标记的抗原（14），并将所得标记的抗原（14）捕获在其上固定有能够特异性结合测试受试者抗原的特异性结合抗体的固定相上。

2. 发明申请

US20100193361A1 APPARATUS FOR CONCENTRATING DIELECTRIC MICROPARTICLES 审中-公开
标题翻译：用于浓缩介电微量计的装置
公开(公告)号：US20100193361A1
公开(公告)日：2010-08-05
申请号：US12602555
申请日：2008-05-30
申请人： Takaharu Enjoji , Satoshi Uchida
发明人： Takaharu Enjoji , Satoshi Uchida
IPC分类号： B01D57/02
CPC分类号： B03C5/026 , B01L3/502753 , B01L3/502761 , C12M47/02 , C12Q1/02 , G01N1/40 , G01N2001/4038
摘要： Microorganisms (dielectric microparticles) in a liquid sample are captured using a dielectrophoretic force. After quantifying and analyzing, the microorganisms thus captured are concentrated and collected. In an apparatus for concentrating dielectric microparticles, a liquid sample, which contains the microorganisms to be examined, is supplied from a liquid sample-holding unit holding the liquid sample and passes through a voltage-applied cell. During the passage, the microorganisms are captured on dielectrophoretic electrodes by a dielectrophoretic force. Then, the captured microorganisms are released from the dielectrophoretic force by ceasing the voltage application. At the same time, a release liquid supplied from a release liquid-holding unit is flown through the dielectrophoretic electrodes so that the concentrated microorganisms are released and collected into a collection unit as the target bacteria.
摘要翻译：使用介电电泳力捕获液体样品中的微生物（电介质微粒）。定量分析后，将如此捕获的微生物浓缩并收集。在用于浓缩电介质微粒的装置中，从保持液体样品的液体样品保持单元提供含有待检测微生物的液体样品，并通过施加电压的电池。在通过期间，通过介电电泳力将微生物捕获在介电电泳电极上。然后，捕获的微生物通过停止施加电压从介电电泳力释放。同时，从释放液体保持单元供给的释放液体通过介电泳电极流动，从而将浓缩的微生物释放并收集到收集单元中作为目标细菌。

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