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1. 发明授权

US4208480A Method, reagents and apparatus for the rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis 失效
标题翻译：用于快速鉴定淋病奈瑟氏球菌和脑膜炎奈瑟菌的方法，试剂和仪器
公开(公告)号：US4208480A
公开(公告)日：1980-06-17
申请号：US936292
申请日：1978-08-23
申请人： Richard F. D'Amato , Louis A. Eriquez
发明人： Richard F. D'Amato , Louis A. Eriquez
IPC分类号： C12M1/20 , C12Q1/12 , C12Q1/04
CPC分类号： C12Q1/12 , Y10S435/871
摘要： The disclosure is directed to a method, reagents and apparatus for the rapid (one to four hours) identification of Neisseria gonorrhoeae and Neisseria meningitidis from cultures grown on a selective or a non-selective medium. One drop of growth suspended in sterile salt solution is placed in each of a plurality of miniature reaction chambers supported on a common base and incubated at 35.degree.-37.degree. C. for one to four hours. A dried substrate and buffer is contained in each chamber. A detector reagent such as a diazo dye in an aqueous solution with a polar solvent is added to each chamber after incubation. The color change in each chamber is noted and compared with a profile to identify N. gonorrhoeae and Neisseria meningitidis. Synthetic substrates rather than sugars are used in the reaction chambers. The substrates are naphthyl derivatives, .beta.-naphthyl or .beta.-naphthylamides, and are(1) .beta.-naphthyl-.beta.,D-galactopyranoside(2) N-L-.lambda.-glutamyl-.beta.-naphthylamide(3) L-hydroxyproline-.beta.-naphthylamide(4) L-serine-.beta.-naphthylamide(5) L-arginine-.beta.-naphthylamide(6) glycine-glycine-.beta.-naphthylamide(7) .beta.-naphthyl-phosphate(8) .beta.-naphthyl-valerate(9) 4 methoxy leucine-.beta.-naphthylamide(10) glycine-.beta.-naphthylamide.
摘要翻译：本公开涉及用于从在选择性或非选择性培养基上生长的培养物快速（一至四小时）鉴定淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌的方法，试剂和装置。将悬浮在无菌盐溶液中的一滴生长物置于支撑在共同碱基上的多个微型反应室中，并在35℃-37℃下孵育1-4小时。干燥的基材和缓冲液包含在每个室中。在温育后，向每个室中加入在具有极性溶剂的水溶液中的重氮染料等检测剂试剂。注意每个室中的颜色变化，并与一个轮廓进行比较，以鉴定淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌。合成底物而不是糖用于反应室。底物是萘衍生物，β-萘基或β-萘基酰胺，并且是（1）β-萘基-β，D-吡喃半乳糖苷（2）NL-λ-谷氨酰基-β-萘基酰胺（3）L-羟基脯氨酸-β-萘基酰胺（4）L-丝氨酸-β-萘基酰胺（5）L-精氨酸-β-萘基酰胺（6）甘氨酸 - 甘氨酸-β-萘基酰胺（7）β-萘基 - 磷酸酯（8）β-萘基 - 戊酸酯（9）4 甲氧基亮氨酸-β-萘基酰胺（10）甘氨酸-β-萘基酰胺。

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