专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10865436B2 Microfluidic apparatus, method, and applications 有权
公开(公告)号：US10865436B2
公开(公告)日：2020-12-15
申请号：US14478012
申请日：2014-09-05
申请人： RHEONIX, INC.
发明人： Peng Zhou
IPC分类号： B01L3/00 , C12Q1/6806 , C12N15/10 , C07H1/08
CPC分类号： C12Q1/6806 , B01L3/502715 , C07H1/08 , C12N15/1003 , B01L2200/0631 , C12Q1/6806 , C12Q2527/101 , C12Q2565/629
摘要： A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.

2. 发明申请

US20190085838A1 CHANNEL-LESS PUMP, METHODS, AND APPLICATIONS THEREOF 审中-公开
公开(公告)号：US20190085838A1
公开(公告)日：2019-03-21
申请号：US16193050
申请日：2018-11-16
申请人： Rheonix, Inc.
发明人： Lincoln C. Young , Peng Zhou
IPC分类号： F04B43/04 , F04B43/06 , F04B43/02 , F04B43/00 , F04B43/12 , B01L3/00
摘要： A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section. A method of transporting fluid using the channel-less microfluidic pump is described.

3. 发明申请

US20150056650A1 MICROFLUIDIC APPARATUS, METHOD, AND APPLICATIONS 审中-公开
标题翻译：微流体装置，方法和应用
公开(公告)号：US20150056650A1
公开(公告)日：2015-02-26
申请号：US14478049
申请日：2014-09-05
申请人： RHEONIX, INC.
发明人： Peng Zhou
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L2200/0631 , C07H1/08 , C12N15/1003 , C12Q2527/101 , C12Q2565/629
摘要： A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
摘要翻译：微流体装置，方法和相关应用利用并应用于福尔马林固定的石蜡包埋（FFPE）组织样品，并且在核酸纯化步骤之前进行液 - 液萃取从组织样品中除去石蜡。微流体装置包括专用液 - 液提取处理容器，核酸纯化工艺组分和核酸扩增反应器。液 - 液萃取和核酸纯化试剂盒包括能够进行液 - 液萃取方法和核酸纯化方法的微流体装置，包括专用液 - 液萃取处理容器，不混溶液体或前体相在容器中，核酸纯化方法组分，流式的核酸扩增反应器和适于实现液 - 液萃取方法和核酸纯化方法的试剂供应。

4. 发明申请

US20140065598A1 SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS 有权
公开(公告)号：US20140065598A1
公开(公告)日：2014-03-06
申请号：US13770521
申请日：2013-02-19
申请人： RHEONIX, INC.
发明人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Greg Mouchka , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6806 , B01L3/022 , B01L3/5027 , B01L3/502753 , B01L7/52 , B01L2200/0647 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/044 , B01L2300/0681 , B01L2300/0829 , B01L2300/0838 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2400/043 , B01L2400/0481 , B01L2400/0666 , C12Q1/6846 , C12Q1/686 , C12Q1/6883 , C12Q1/708 , G01N35/1002 , C12Q2565/629
摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

5. 发明申请

US20130157885A1 SYSTEM AND METHODS FOR SELECTIVE MOLECULAR ANALYSIS 审中-公开
标题翻译：用于选择性分子分析的系统和方法
公开(公告)号：US20130157885A1
公开(公告)日：2013-06-20
申请号：US13680231
申请日：2012-11-19
申请人： RHEONIX, INC.
发明人： Gwendolyn Spizz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6832 , C12Q1/6848 , C12Q1/6858 , C12Q2525/113 , C12Q2531/113 , C12Q2525/101 , C12Q2525/107 , C12Q2565/514
摘要： Methods and systems for selectively amplifying a target DNA sequence in the presence of non-target DNA sequence in a sample, comprising: contacting the sample with an oligonucleotide system under hybridization conditions to form a reaction mixture including a forward primer and a reverse primer, wherein either the forward or reverse primer is modified to preferentially increase hybridization between the primer and the target sequence; cycling the hybridization of the oligonucleotide system so that, if the target DNA sequence is present in the sample, the primers hybridize to the target DNA sequence and the reaction mixture results in a first amplified product; and detecting the first amplified product.
摘要翻译：在样品中非靶DNA序列存在下选择性扩增靶DNA序列的方法和系统，包括：在杂交条件下使样品与寡核苷酸系统接触以形成包含正向引物和反向引物的反应混合物，其中修饰前向或反向引物以优先增加引物和靶序列之间的杂交; 循环寡核苷酸系统的杂交，使得如果目标DNA序列存在于样品中，则引物与靶DNA序列杂交，并且反应混合物产生第一扩增产物; 并检测第一个扩增产物。

6. 发明授权

US10180133B2 Channel-less pump, methods, and applications thereof 有权
公开(公告)号：US10180133B2
公开(公告)日：2019-01-15
申请号：US14548474
申请日：2014-11-20
申请人： Rheonix, Inc.
发明人： Lincoln C. Young , Peng Zhou
IPC分类号： F04B43/12 , F04B43/04 , F04B43/06 , B01L3/00 , F04B43/00 , F04B43/02
摘要： A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section. A method of transporting fluid using the channel-less microfluidic pump is described.

7. 发明申请

US20180327817A1 Strain Typing Assay and Method Without Need for Isolation in Pure Form and Subsequent Longitudinal Strain Tracking 审中-公开
公开(公告)号：US20180327817A1
公开(公告)日：2018-11-15
申请号：US15978333
申请日：2018-05-14
申请人： Rheonix, Inc.
发明人： F. Morgan Wallace
IPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/6874 , C12Q1/6837 , G06K9/62
CPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , C12Q2600/156 , G06K9/6227 , G06K9/6255 , G06K9/6276 , G06K9/6292 , C12Q2565/50
摘要： A system and method for strain typing without need for isolation in pure form and subsequent longitudinal strain tracking. The method includes the steps of locating one or more genetic regions present in a target. The genetic regions contain genetic loci that vary among two or more variants (i.e., strains) of the target. A device detects a unique sequence of each of the genetic loci. After a sample of biological material having the genetic loci is obtained, the device generates an amplicon for the genetic regions present in the target. The amplicons are hybridized to complimentary probes, resulting in hybridized probes and non-hybridized probes, which are detected. The detected hybridized probes are assigned an identifier. The device transforms the identifiers into a pattern. The pattern is recorded and compared to one or more other patterns recorded to determine if the pattern is different from the one or more other patterns.

8. 发明申请

US20160222435A1 Self-contained Biological Assay Apparatus, Methods, and Applications 有权
标题翻译：独立生物测定仪器，方法和应用
公开(公告)号：US20160222435A1
公开(公告)日：2016-08-04
申请号：US15061478
申请日：2016-03-04
申请人： Rheonix, Inc.
发明人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin , Greg Mouchka
IPC分类号： C12Q1/68 , C12Q1/70 , B01L7/00 , G01N35/10 , B01L3/00
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/10 , B01L2200/14 , B01L2300/0627 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/18 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , C12Q1/6809 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C12Q1/6846 , C12Q1/6876 , C12Q1/708 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , G01N35/10 , G01N35/1002 , G01N2035/00346 , C12Q2565/629
摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
摘要翻译：独立的，完全自动化的生物测定执行装置包括壳体; 包括设置在所述壳体中的可控制的试剂分配系统的分配平台; 设置在所述壳体中的试剂供应部件; 气动歧管，其可移除地设置在所述壳体中的由所述分配平台共享的空间中，可拆卸地联接到流体输送层和多个储存器，其中所述流体输送层，所述储存器以及待引入其中的待测样品设置在空间中的壳体与分配平台分离; 气动供给系统，其可拆卸地联接到与分配平台分离的空间中的壳体中的气动歧管; 以及耦合到所述分配平台和所述气动供应系统中的至少一个的控制系统，所述控制系统设置在所述壳体中。

9. 发明申请

US20150307921A1 SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS 有权
标题翻译：自包含生物测定装置，方法和应用
公开(公告)号：US20150307921A1
公开(公告)日：2015-10-29
申请号：US14798719
申请日：2015-07-14
申请人： RHEONIX, INC.
发明人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin , Greg Mouchka
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/10 , B01L2200/14 , B01L2300/0627 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/18 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , C12Q1/6809 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C12Q1/6846 , C12Q1/6876 , C12Q1/708 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , G01N35/10 , G01N35/1002 , G01N2035/00346 , C12Q2565/629
摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
摘要翻译：独立的，完全自动化的生物测定执行装置包括壳体; 包括设置在所述壳体中的可控制的试剂分配系统的分配平台; 设置在所述壳体中的试剂供应部件; 气动歧管，其可移除地设置在所述壳体中的由所述分配平台共享的空间中，可拆卸地联接到流体输送层和多个储存器，其中所述流体输送层，所述储存器以及待引入其中的待测样品设置在空间中的壳体与分配平台分离; 气动供给系统，其可拆卸地联接到与分配平台分离的空间中的壳体中的气动歧管; 以及耦合到所述分配平台和所述气动供应系统中的至少一个的控制系统，所述控制系统设置在所述壳体中。

10. 发明授权

US09134207B2 Microfluidic chips and assay systems 有权
标题翻译：微流控芯片和测定系统
公开(公告)号：US09134207B2
公开(公告)日：2015-09-15
申请号：US14268399
申请日：2014-05-02
申请人： RHEONIX, INC.
发明人： Peng Zhou , Lincoln C. Young
IPC分类号： G01N1/28 , B01L3/00 , F04B43/04 , F16K99/00 , B01L7/00
CPC分类号： G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
摘要： The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
摘要翻译：本文所述的系统和方法包括具有互连以提供用于处理至少一种试剂的可配置流体输送系统的多个微特征的微流体芯片。提供插入件可移除地与芯片的一个或多个微特征相配合，其中插入物包括与试剂相互作用的位点。从以下描述可以看出，微流体芯片和插入物提供用于进行平行测定的有效和准确的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式